10 minutes assay of Oxidative Stress by Elleman's Method Metallogenics' Thiol Detection Assay Kit

In living organisms, thiol groups exist as free cysteine, glutathione, cysteine residues in proteins (such as albumin). Thiol groups attached to a carbon atom have a strong reducing ability, and plays an important role for the antioxidant capacity to scavenge ROS. According to a report, patients of various diseases exhibit low level of serum protein thiols. Furthermore, the oxidations of protein thiol groups contribute to the tertiary or quaternary structure of a protein.

Metallogenics' thiol detection assay kit (Ellman's method) measures total thiol groups in blood samples. Ellman's reagent (DTNB: 5,5'-Dithiobis(2-nitrobenzoic acid)) reacts with thiol groups in blood samples, and yields TNB2- (5-Mercapto-2-nitrobenzoic acid), showing yellow color. The intensity of the color is proportional to the thiol groups in blood samples. Thiol concentration can be calculated by measuring absorbance of TNB2- at wavelength 412 nm (380 nm to 440 nm).

Ellman's reagent (DTNB:5,5'-Dithiobis (2-nitrobenzoic acid)) reacts with thiol group and produces 5-Mercapto-2-nitrobenzoic acid (TNB). Measure absorbance at λmax=412 nm and quantify thiol concentration.