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10 minutes assay of Oxidative Stress by Elleman's Method Metallogenics' Thiol Detection Assay Kit

Date:October 23 2014Web Page No:80445

In living organisms, thiol groups exist as free cysteine, glutathione, cysteine residues in proteins (such as albumin). Thiol groups attached to a carbon atom have a strong reducing ability, and plays an important role for the antioxidant capacity to scavenge ROS. According to a report, patients of various diseases exhibit low level of serum protein thiols. Furthermore, the oxidations of protein thiol groups contribute to the tertiary or quaternary structure of a protein.

Metallogenics' thiol detection assay kit (Ellman's method) measures total thiol groups in blood samples. Ellman's reagent (DTNB: 5,5'-Dithiobis(2-nitrobenzoic acid)) reacts with thiol groups in blood samples, and yields TNB2- (5-Mercapto-2-nitrobenzoic acid), showing yellow color. The intensity of the color is proportional to the thiol groups in blood samples. Thiol concentration can be calculated by measuring absorbance of TNB2- at wavelength 412 nm (380 nm to 440 nm).

Metallogenics' Thiol Detection Assay Kit

Features

  • Rapid assay (Just 10 minutes)
  • Simple Protocol
  • No hazardous components such as cyanide or azide as preservative
  • Compatible with multi-species
  • Samples : Serum, Plasma
  • 2 points calibration

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Principle of Ellman's method

Ellman's reagent (DTNB:5,5'-Dithiobis (2-nitrobenzoic acid)) reacts with thiol group and produces 5-Mercapto-2-nitrobenzoic acid (TNB). Measure absorbance at λmax=412 nm and quantify thiol concentration.

Principle of Ellman's method

[Date : October 31 2020 00:22]

Detail Product Name Product Code Supplier Size Price
Thiol Detection Assay kit <100tests>
DatasheetThis may not be the latest data sheet.
TH01DE AKJMetallogenics Co.,Ltd. 1 kit $430

Description Thiol Detection Assay kit measures SH amount in sample by DTNB (Ellman) method.
Storage 4°C CAS
Link

[Date : October 31 2020 00:22]

Thiol Detection Assay kit <100tests>


  • Product Code: TH01DE
  • Supplier: AKJ
  • Size: 1kit
  • Price: $430

Description Thiol Detection Assay kit measures SH amount in sample by DTNB (Ellman) method.
Storage 4°C CAS
Link

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