Nitroso-PSAP method Iron Concentration Assay Kit Iron Assay kit LS | Metallo Assay LS
Date：June 11 2020Web Page No：80414
Metallo Assay Iron Assay Kit LS (Nitroso-PSAP Method) can measure Iron ion (Fe2+, Fe3+) in the biological samples such as Plasma, Serum, Urine, and wide range of samples by microplate reader.
Features
- Using microplate reader for assay, no need to use Atomic Absorption Spectrometry or ICP-OES Optical Emission Spectrometry, which have been mainly used so far.
- Good correlation with the result of Atomic Absorption Spectrometry and ICP-OES.
- High throughput measurement by using microplate reader.
- Assay range: 10-1,000 μg/dL
- All animal species sample can be measured.
- Assay time : just 10 minutes!
- 1 point calibration – do not need multiple point measurement to make standard curve (r^2=0.9999).
- Kit does not contain any hazardous components like cyanide or azide as preservative.
- Samples : Serum, Plasma, Urine, Saliva, Cell lysate, Tissue extract, Hair extract, Water, etc
- In combination with UIBC Assay kit LS Bathophenanthroline method (#UIB01E), TIBC (Total Iron Binding Capacity) can be measured.
Assay Principle
This kit measures iron concentration by color change caused by chelate complex formulation of Nitroso-PSAP and Iron. Iron combined to transportation protein such as transferrin is denatured by weak acid and denaturant then formulates Iron-Nitroso-PSAP chelate complex. Measuring this complex by 750 nm wavelength and obtain iron concentration.
Sample preparation
- Samples which do not require preparation
e.g. Serum, Plasma and Urine
* EDTA treated sample is not suitable for this assay.
* Pooled urine should add HCl for storing. When assay, adujust sample pH 2 to 3. - Samples which require acid extraction
e.g. cell lysate, tissue homogenate and other liquid samples.
* Measurable chemical species is limited as follows:
・Free iron
・Iron binds to protein (Protein-Fe2+, e.g. transferrin, ferritin, metallothionein)
* Lysis buffer contains EDTA is not suitable for this assay.
* Organoiron and Heme iron requires acidolysis. - Microwave method and samples require acidolysis
e.g. samples contain strong chelate agents such as EDTA, Organoiron (in the case of single bond -C-Fe-C-),
iron in cyclic ligand (Fe-Porphyrin)
* After acidolysis, adjust sample pH in 2 to 3.
Handling procedure
| (1)Apply DW, Standard and Sample (15 uL) to 96 well plate. |
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| (2)Add R-A buffer (160 uL). |
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| (3)Incubate at room temperature for 10 minutes. |
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| (4)Add working reagent (75 uL). |
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| (5)Incubate at room temperature for 5 minutes. |
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| (6)Read the absorbance at 750 nm (main). |
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| (7)Calculate concentration. ΔODStd = ODStd - ODBl, ΔODS = ODS - ODBl |
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Price
[Date : July 09 2025 00:07]
| Detail | Product Name | Product Code | Supplier | Size | Price | ||||||||||||||||||||||||||||||
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Iron Assay kit LS - Nitroso-PSAP method DatasheetThis may not be the latest data sheet. |
FE02ME | AKJMetallogenics Co.,Ltd. | 1 kit | $296 | |||||||||||||||||||||||||||||||
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[Date : July 09 2025 00:07]
Iron Assay kit LS - Nitroso-PSAP method
DatasheetThis may not be the latest data sheet.
- Product Code: FE02ME
- Supplier: AKJ
- Size: 1kit
- Price: $296
| Description |
Metallo Assay Iron Assay kit LS is intended for the quantitative determination of iron (: free iron , non-heme iron) in biological sample by 96-well reader use. Target: free iron (Fe2+ and Fe3+) Measuring range: 10-1,000 μg/Dl Species: all Sample: serum, plasma, urine, saliva, tissue extract, cell lysate, biological fluid, food, beverage etc. Apparatus: UV/Vis 96-well reader or spectrophotometer Wavelength: 750 nm (740-760nm) Procedure: 10 min |
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| Storage | 4°C | CAS | |
| Link |
Assay Kit Product List |
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Citations
Hydroxyl radicals cause fluctuation in intracellular ferrous ion levels upon light exposure during photoreceptor cell death.
Exp Eye Res ; October 24, 2014
JAK2V617F-positive myeloproliferative neoplasm clones evoke paracrine DNA damage to adjacent normal cells through secretion of lipocalin-2. Blood ; September 12, 2014
Angiotensin II alters the expression of duodenal iron transporters, hepatic hepcidin, and body iron distribution in mice.
Eur J Nutr ; August 7, 2014
Iron Chelation by Deferoxamine Prevents Renal Interstitial Fibrosis in Mice with Unilateral Ureteral Obstruction.
PLoS One ; February 19, 2014
TEMPO-oxidized cellulose hydrogel as a high-capacity and reusable heavy metal ion adsorbent.
J Hazard Mater ;September 15, 2013
Superparamagnetic nanoparticle clusters for cancer theranostics combining magnetic resonance imaging and hyperthermia treatment. Theranostics ; April 23, 2013
Dietary iron restriction inhibits progression of diabetic nephropathy in db/db mice.
Am J Physiol Renal Physiol ; April 1, 2013
GRK6 deficiency in mice causes autoimmune disease due to impaired apoptotic cell clearance.
Nat Commun ;February 26, 2013
Estrogen Regulates Hepcidin Expression via GPR30-BMP6-Dependent Signaling in Hepatocytes.
PLoS One ; July 11, 2012
FecA1, a bacterial iron transporter, determines the survival of Helicobacter pylori in the stomach.
Free Radic Biol Med ; March 15, 2012
Iron is contained in various enzymes as an important constituent element. Iron in the blood are all combined to transferrin, and transported to erythroblast and tissues for synthesis of globin proteins such as myoglobin and hemoglobin which requests iron. Iron is essential for generation of oxigen transporting proteins. Deficient of such proteins cause hypoferric anemia, chronic bleeding anemia, and infectious anemia. Increase of transferrin and high concentration of iron are reported in hepatitis and liver cirrhosis.Increase of iron is reported in Hypoplastic anemia and malignant anemia.
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