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HOME> Products> Instrumentation> ZF Plate

96 well microplate for easy alignment of zebrafish ZF Plate

Date：February 24 2016Web Page No：80394

Appearance of 96 well microplate that efficiently aligns zebrafish

This 96 well plate is optimized for drug discovery screening using zebrafish. Zebrafish can be easily aligned simply by placing them in each well and centrifuging them.
In addition, the glass bottom plate with excellent optical properties makes chemical compound screening using zebrafish more efficient and quantitative.
The thin bottom glass surface enables clear confocal microscopy.

※ Learn more about Gemma Micro ZF, a feed that supports all life stages of zebrafish (scientific name: Danio rerio).

Features
Specifications
Example Data
Example Protocol
Compatible Devices
Product information

Features

Zebrafish can be easily aligned by putting into each well and centrifuging them.
Alignment rate* is more than 70%.
Glass bottom plate with excellent optical properties enables more efficient and quantitative chemical-screening using zebrafish.
Fluorescence and bright-field imaging are supported.
Zebrafish quantitative image phenotype analysis is possible by using a high-content imaging system.
By aligning zebrafish, it is possible to obtain analysis results with high S/N ratio.
The thin bottom glass allows for clearer confocal microscopy.

* The alignment ratio means "Percentage of animals in a horizontal row that can be photographed from the side of the body."
※ This plate is disposable.
※ Non–Autoclavable.

(Left) Fluorescent image
(Right) Bright field image

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Specifications

Plate size (mm)	85.48 (L) x 127.76 (W) x 14.35 (H)
Plate material / Color	Polystyrene / Black
Bottom material / Thickness	Silica glass / 0.17 mm
Weight	Without-cover: 72 g With-cover: 95 g
Temperature for use	0-40 ℃
Maximum loading volume per well	250 μL
Sterilized / Unsterilized	Unsterilized

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Example Data

High content imager using ZF plate and a conventional microplate
ZF Plate Example Data

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Example Protocol

Sample

Anesthetized zebrafish (young fish, approx. 3–6 dpf )

Materials needed but not supplied

Microplate centrifuge (with flash function)
Micropipette (100–200 μL and 1 mL)
Pipet tip (wide mouth and low adsorption type recommended)

Protocol

Pipette a zebrafish with anesthetic solution (approx. 75 μL) and gently place into a well.
Quick spin down microplate with centrifuge (200-250G).
Scan with a compatible imaging device (see below).
After scanning, add additional anesthetic solution and remove zebrafish with micropipette for further experiments.

You can check the process in the video!

※ About anesthesia
Y, Ashikawa., et al., Pharmaceuticals., 12(4), 156 (2019). [PMID: 31623237]
D, Yamamoto., et al., Zebrafish, 16(3), 233-239 (2019). [PMID: 30855222]

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Compatible Devices

Molecular Devices	ImageXpress Micro XLS ImageXpress Velos
Essen Instruments	IncuCyte
Bio-Rad	Ultramark
GE	IN Cell Analyzer 1000 IN Cell Analyzer 3000
Li-Cor	Aerius
Life Technologies	EVOS Auto Imaging System EVOS FL Imaging System EVOS XL Core Imaging Systems
PerkinElmer	ViewLux
Idea Bio-medical	Hermes

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Product information

[Date : April 25 2025 00:06]

Detail Product Name Product Code Supplier Size Price

ZF Plate Ver.2
DatasheetThis may not be the latest data sheet. HDK-ZFA101-02a HDK 5 pieces $518

Contact

Description	ZF Plate is 96 well plate for drug discovery screening by using zebrafish. You can easily align zebrafishes just put them into well and spin down.
Storage		CAS
Link

ZF Plate Ver.2
DatasheetThis may not be the latest data sheet. HDK-ZFA101-02a-s HDK 1 piece $104

Contact

Description	ZF Plate is 96 well plate for drug discovery screening by using zebrafish. You can easily align zebrafishes just put them into well and spin down.
Storage		CAS
Link

[Date : April 25 2025 00:06]

ZF Plate Ver.2

DatasheetThis may not be the latest data sheet.

Product Code: HDK-ZFA101-02a
Supplier: HDK
Size: 5pieces
Price: $518

Description	ZF Plate is 96 well plate for drug discovery screening by using zebrafish. You can easily align zebrafishes just put them into well and spin down.
Storage		CAS
Link

Contact

ZF Plate Ver.2

DatasheetThis may not be the latest data sheet.

Product Code: HDK-ZFA101-02a-s
Supplier: HDK
Size: 1piece
Price: $104

Description	ZF Plate is 96 well plate for drug discovery screening by using zebrafish. You can easily align zebrafishes just put them into well and spin down.
Storage		CAS
Link

Contact

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CONTACT

export@funakoshi.co.jp

※Prices on our website are for your reference only. Please inquire your distributor for your prices.
※Please note that Product Information or Price may change without notice.