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Total Antioxidant Capacity (TAC) Assay Kit

Date:January 01 2016Web Page No:80379

Rapid (10 minutes) High Throughput Screening Assay Kit for Total Antioxidant Capacity (TAC) !

Features and Benefits

  • Copper Reduction Ability assay method
  • Just 10 minutes assay
  • Kit does not contain any hazardous components like cyanide or azide as preservative.
  • Samples from any animal species can be measured.
  • 2 points calibration - does not require multiple point measurement to make standard curve
  • Samples : Serum, Plasma, Urine, foods such as wine, beverage and tea, etc.
  • Simple Protocol

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Protocol

Protocol

Calculate TAC (copper reduction ability) of the samples by comparing the ΔOD of the samples to the ΔOD of standard
(1 mM ascorbic acid).

ΔODstandard = (OD2standard – OD1standard) – (OD2blank – OD1blank)
ΔODsample = (OD2sample – OD1sample) – (OD2blank – OD1blank)

Copper reduction ability
TAC (mM) = (ΔODstandard / ΔODsample) x 2

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Kit Components

  • R-A : Buffer solution (bathocuproine)
  • R-B : Cupric ion (Cu2+) solution
  • R-C : Stop solution
  • Standard (STD) : Calibrator (Ascorbic acid 1.0mM)

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Example

Fig.1 Total antioxidant capacities of various antioxidants

Fig.1 Total antioxidant capacities of various antioxidants

Fig.2 Total antioxidant capacities in bovine serum

Fig.2 Total antioxidant capacities in bovine serum

(Note)

  • Remove insoluble substances in serum and plasma samples by filtration or centrifugation.
  • Hemolyzed sample cannot be used.
  • Do not use EDTA for anticoagulant.
  • Please use same type of blood collection tubes when TACs in plasma samples was compared.

(Some of anticoagulant has reduction ability.)

  • Samples must be assayed fresh. If you want to store the samples, please keep in -20℃ and do not repeat freeze/ thawing cycle that causes damage to the samples.

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Reference

  • Campos C, Guzmán R, López-Fernández E, Casado A.
    “Evaluation of the copper(II) reduction assay using bathocuproinedisulfonic acid disodium salt for the total antioxidant capacity assessment: the CUPRAC-BCS assay.”
    Anal Biochem. 2009 Sep 1;392(1):37-44. doi: 10.1016/j.ab.2009.05.024. Epub 2009 May 21.

  • Yuji Naito, Masaichi-Chang-il Lee, Yoji Kato, Ryoji Nagano, Yoshikazu Yonei
    “Oxidative Stress Markers.” Anti-Aging Medicine 7(5):36-44,2010

[Date : September 24 2020 23:16]

Detail Product Name Product Code Supplier Size Price
Total Antioxidant Capacity Assay Kit <200tests>
DatasheetThis may not be the latest data sheet.
AC01DE AKJMetallogenics Co.,Ltd. 1 kit $600

Description Total Antioxidant Capacity Assay Kit measures TAC:Total Antioxidant Capacity in sample.
Storage 4°C CAS
Link

[Date : September 24 2020 23:16]

Total Antioxidant Capacity Assay Kit <200tests>


  • Product Code: AC01DE
  • Supplier: AKJ
  • Size: 1kit
  • Price: $600

Description Total Antioxidant Capacity Assay Kit measures TAC:Total Antioxidant Capacity in sample.
Storage 4°C CAS
Link

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CONTACT

export@funakoshi.co.jp

  • Prices are for USA / Canada customers. Prices do not include shipping and handling charges, VAT, import tariffs and service charge etc.
  • Please note that Product Information or Price may change without notice.