Total Antioxidant Capacity (TAC) Assay Kit
Date:January 01 2016Web Page No:80379
Rapid (10 minutes) High Throughput Screening Assay Kit for Total Antioxidant Capacity (TAC) !
Features and Benefits
- Copper Reduction Ability assay method
- Just 10 minutes assay
- Kit does not contain any hazardous components like cyanide or azide as preservative.
- Samples from any animal species can be measured.
- 2 points calibration - does not require multiple point measurement to make standard curve
- Samples : Serum, Plasma, Urine, foods such as wine, beverage and tea, etc.
- Simple Protocol
Protocol

Calculate TAC (copper reduction ability) of the samples by comparing the ΔOD of the samples to the ΔOD of standard
(1 mM ascorbic acid).
ΔODstandard = (OD2standard – OD1standard) – (OD2blank – OD1blank)
ΔODsample = (OD2sample – OD1sample) – (OD2blank – OD1blank)
Copper reduction ability
TAC (mM) = (ΔODstandard / ΔODsample) x 2
Kit Components
- R-A : Buffer solution (bathocuproine)
- R-B : Cupric ion (Cu2+) solution
- R-C : Stop solution
- Standard (STD) : Calibrator (Ascorbic acid 1.0mM)
Example

Fig.1 Total antioxidant capacities of various antioxidants

Fig.2 Total antioxidant capacities in bovine serum
(Note)
- Remove insoluble substances in serum and plasma samples by filtration or centrifugation.
- Hemolyzed sample cannot be used.
- Do not use EDTA for anticoagulant.
- Please use same type of blood collection tubes when TACs in plasma samples was compared.
(Some of anticoagulant has reduction ability.)
- Samples must be assayed fresh. If you want to store the samples, please keep in -20℃ and do not repeat freeze/ thawing cycle that causes damage to the samples.
Reference
Campos C, Guzmán R, López-Fernández E, Casado A.
“Evaluation of the copper(II) reduction assay using bathocuproinedisulfonic acid disodium salt for the total antioxidant capacity assessment: the CUPRAC-BCS assay.”
Anal Biochem. 2009 Sep 1;392(1):37-44. doi: 10.1016/j.ab.2009.05.024. Epub 2009 May 21.Yuji Naito, Masaichi-Chang-il Lee, Yoji Kato, Ryoji Nagano, Yoshikazu Yonei
“Oxidative Stress Markers.” Anti-Aging Medicine 7(5):36-44,2010
[Date : February 09 2025 00:06]
Detail | Product Name | Product Code | Supplier | Size | Price | ||||||||||||||||||||||||||||||
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Total Antioxidant Capacity Assay Kit <200tests> DatasheetThis may not be the latest data sheet. |
AC01DE | AKJMetallogenics Co.,Ltd. | 1 kit | $482 | |||||||||||||||||||||||||||||||
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[Date : February 09 2025 00:06]
Total Antioxidant Capacity Assay Kit <200tests>
DatasheetThis may not be the latest data sheet.
- Product Code: AC01DE
- Supplier: AKJ
- Size: 1kit
- Price: $482
Description |
Total Antioxidant Capacity Assay Kit measures TAC:Total Antioxidant Capacity in sample. |
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Storage | 4°C | CAS | |
Link |
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CONTACT
export@funakoshi.co.jp
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