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HOME> Products> Assay Kits> Total Antioxidant Capacity (TAC) Assay Kit

Total Antioxidant Capacity (TAC) Assay Kit

Date：January 01 2016Web Page No：80379

Metallogenics Co.,Ltd.

Rapid (10 minutes) High Throughput Screening Assay Kit for Total Antioxidant Capacity (TAC) !

Features and Benefits
Protocol
Kit Components
Example
Reference

Features and Benefits

Copper Reduction Ability assay method
Just 10 minutes assay
Kit does not contain any hazardous components like cyanide or azide as preservative.
Samples from any animal species can be measured.
2 points calibration - does not require multiple point measurement to make standard curve
Samples : Serum, Plasma, Urine, foods such as wine, beverage and tea, etc.
Simple Protocol

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Protocol

Calculate TAC (copper reduction ability) of the samples by comparing the ΔOD of the samples to the ΔOD of standard
(1 mM ascorbic acid).

ΔODstandard = (OD2standard – OD1standard) – (OD2blank – OD1blank)
ΔODsample = (OD2sample – OD1sample) – (OD2blank – OD1blank)

Copper reduction ability
TAC (mM) = (ΔODstandard / ΔODsample) x 2

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Kit Components

R-A : Buffer solution (bathocuproine)
R-B : Cupric ion (Cu2+) solution
R-C : Stop solution
Standard (STD) : Calibrator (Ascorbic acid 1.0mM)

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Example

Fig.1 Total antioxidant capacities of various antioxidants

Fig.2 Total antioxidant capacities in bovine serum

(Note)

Remove insoluble substances in serum and plasma samples by filtration or centrifugation.
Hemolyzed sample cannot be used.
Do not use EDTA for anticoagulant.
Please use same type of blood collection tubes when TACs in plasma samples was compared.

(Some of anticoagulant has reduction ability.)

Samples must be assayed fresh. If you want to store the samples, please keep in -20℃ and do not repeat freeze/ thawing cycle that causes damage to the samples.

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Reference

Campos C, Guzmán R, López-Fernández E, Casado A.
“Evaluation of the copper(II) reduction assay using bathocuproinedisulfonic acid disodium salt for the total antioxidant capacity assessment: the CUPRAC-BCS assay.”
Anal Biochem. 2009 Sep 1;392(1):37-44. doi: 10.1016/j.ab.2009.05.024. Epub 2009 May 21.
Yuji Naito, Masaichi-Chang-il Lee, Yoji Kato, Ryoji Nagano, Yoshikazu Yonei
“Oxidative Stress Markers.” Anti-Aging Medicine 7(5):36-44,2010

[Date : February 15 2024 12:37]

Detail Product Name Product Code Supplier Size Price

Total Antioxidant Capacity Assay Kit <200tests>
DatasheetThis may not be the latest data sheet. AC01DE AKJMetallogenics Co.,Ltd. 1 kit $531

Contact

Description	Total Antioxidant Capacity Assay Kit measures TAC：Total Antioxidant Capacity in sample.
Storage	4°C	CAS
Link

[Date : February 15 2024 12:37]

Total Antioxidant Capacity Assay Kit <200tests>

DatasheetThis may not be the latest data sheet.

Product Code: AC01DE
Supplier: AKJ
Size: 1kit
Price: $531

Description	Total Antioxidant Capacity Assay Kit measures TAC：Total Antioxidant Capacity in sample.
Storage	4°C	CAS
Link

Contact

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CONTACT

export@funakoshi.co.jp

※Prices are for USA / Canada customers. Prices do not include shipping and handling charges, VAT, import tariffs and service charge etc.
※Please note that Product Information or Price may change without notice.