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Supporting Reagent for Sectioning Floating Cells/3D Cultured Cells iPGell

Date：December 01 2014Web Page No：62940

Making a section of floating cells or 3D cultured cells (such as embryoid body, spheroid, etc) is burdensome work for researchers.
Conventional methods are cytospin, smear slide, or agarose gel but these method gives damages to your cell sample as the cells are dried or heated during sample preparaiton.
Moreover, for cytospin or smear slide method, you need to start again from sample preparation.

iPGell will greatly help you to achieve this just by using two components.
Cells can be rapidly jellified with unheated condition. Any toxic components affect to cells are not contained.
Jellified sample can be soaked into fixation solution and then you can make a sample block (frozen / paraffin) like the way to make tissue block.

Features
Procedure
Samples
Experiment Examples
Citation

Features

Rapid and Easy procedure
- Just mixing solution A and B, ~2 minutes protocol.
Lower damage to Cells (RNA and Protein)
- as sample is not heated.
Various analysis can be done from same sample
- frozen/paraffin blocks enable you to make a section when you need.

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Procedure

Unique Reagent for Sectioning Floating Cells/3D Cultured Cells iPGel

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Samples

Primary cultured cell
Embryoid body
Spheroid
iPS cells / ES cells
Specific cell population sorted by cell sorter

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Experiment Examples

Morphorogy of mouse derived embryoid body by HE staining


Embryoid body A (x100)	Embryoid body A (x400)	Embryoid body B (x100)	Embryoid body C (x100)

Expression analysis of 3D cultured (spheroid culture) cell

IHC (Fixed frozen section, x 1000)
Antibody D (Brown) / Counter Stain (Blue)

Expression analysis of cultured cell by in situ hybridization

Gene (Blue) / Counter Stain (Pink)	Gene (Blue) / Counter Stain (Pink)	Gene (Blue) / Counter Stain (Pink)

Gene E (x400)	Gene F (x400)	Negative Control (x400)

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Citation

[Date : February 15 2024 12:37]

Detail Product Name Product Code Supplier Size Price

iPGell *MOQ:5*
DatasheetThis may not be the latest data sheet. PG20-1 GNSGenoStaff Co.,Ltd. 1 kit $482

Contact

Description	iPGell is a kit that enables to make cells into a form of gel as "live cells" from cell suspension, 3D cell culture and extremely small samples in same one tube., since the gel is formed through non-thermal process and within a few minutes. The gel can be soaked to a fixative in the same tube, and then made into a block and a number of sections in the same way as tissue samples.
Storage	-20°C	CAS
Link

[Date : February 15 2024 12:37]

iPGell *MOQ:5*

DatasheetThis may not be the latest data sheet.

Product Code: PG20-1
Supplier: GNS
Size: 1kit
Price: $482

Description	iPGell is a kit that enables to make cells into a form of gel as "live cells" from cell suspension, 3D cell culture and extremely small samples in same one tube., since the gel is formed through non-thermal process and within a few minutes. The gel can be soaked to a fixative in the same tube, and then made into a block and a number of sections in the same way as tissue samples.
Storage	-20°C	CAS
Link

Contact

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CONTACT

export@funakoshi.co.jp

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※Please note that Product Information or Price may change without notice.