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Ideal for low molecular weight DNA separation! Newly developed hybrid agarose gel -- Beyond Agarose --
Date:March 19 2026Web Page No:520123
Beyond Agarose is a newly developed hybrid agarose gel designed as a high‑performance alternative to conventional low‑melting‑point agarose widely used for low–molecular-weight DNA separation.
It enables the separation of DNA fragments ranging from 20 bp to 10 kbp with excellent clarity and reproducibility by constructing a mixture of middle to high melting temperature agarose (gel strength (1.5%) ≥ 1,200 g/cm2) and high molecular weight natural hydrophilic compounds.
Specification
- A cost per gel is lower than a conventional low-melting-point agarose widely used for low-molecular-weight DNA separation.
- There is no need to change protocols such as dissolving in an electrophoresis buffer, post-electrophoresis DNA staining, or extraction of DNA fragments from gels.
- The gels made from Beyond Agarose have enhanced mechanical strength and are easy to handle during experiments.
- The gels also have superior transparency, allowing for improved signals during observation.
Example of use
Beyond Agarose can be used with the same protocol of conventional agarose for electrophoresis. Please refer to the product manual (datasheet) which can be downloaded here.
- Measure required amount of Beyond Agarose powder, add to an electrophoresis buffer (* 0.5 x TBE (Tris-borate- EDTA) buffer), and mix well.
*While TAE (Tris-acetate-EDTA) or TPE (Tris-phosphate-EDTA) buffers can be used for electrophoresis, TBE (Tris-borate-EDTA) provides better resolution than the others. - Heat the suspension in a microwave oven until the agarose is completely dissolved.
If necessary, replenish evaporated water with deionized water. - Allow the solution to cool to approximately 50-70℃, then pour the warm agarose solution into the gel mold.
Gently tilt the tray to ensure that the solution is evenly distributed.
Set the appropriate comb into the warm gel and remove bubbles if necessary. - Incubate the gel at room temperature for 30 minutes, then refrigerate for 10 minutes to allow it to solidify completely.
- After warming the gel at room temperature, then, add sufficient electrophoresis buffer (0.5×TBE in this case) to cover the gel completely.
To avoid damaging the wells, gently wiggle the comb back and forth, then slowly lift it straight up to remove the comb. - Load the samples into the wells and run the gel at 100 V for 35–40 minutes.
- Stain the gel with appropriate concentration of ethidium bromide or alternative fluorescent dyes for 15 minutes at room temperature.
- Wash the gel by clean water and observe DNA bands by UV transilluminator.
- Dispose of staining solutions and used gels in accordance with institutional safety regulations.
Application note
Examples of DNA separation at different concentration gels (0.6~1.5 %) using Beyond Agarose (8 cm gels, 100 V, 65 min) in 0.5 x TBE.
50 bp DNA ladder fragments were sufficiently separated in low concentration (0.6%) gel.
In addition, 20 bp DNA ladder fragments were also separated clearly in 1.5 % gel.
DNA size marker: 1) 0.1~20 kb, 2) 0.1~10 kbp, 3) 100 bp ladder marker, 4) 50 bp ladder marker, 5) 20 bp ladder marker
Product Information
[Date : March 22 2026 00:07]
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Beyond Agarose |
GB-BA025 | GBO | 25 g | - | |||||||||||||||||||||||||||||||
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[Date : March 22 2026 00:07]
Beyond Agarose
- Product Code: GB-BA025
- Supplier: GBO
- Size: 25g
- Price: -
| Description |
Beyond Agarose is a newly developed hybrid agarose gel designed as a high‑performance alternative to conventional low‑melting‑point agarose widely used for low–molecular-weight DNA separation. It allows for use in the same manner as conventional agarose. |
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