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Useful tools for regenerative medicine, cell therapy, and gene therapy! Serum-free, basal medium for HSC expansion from Human/Mouse - HemExTM -

Date:June 09 2025Web Page No:520086

Cell Science & Technology Institute, Inc.

HemExTM media are serum-free, and chemically-defined media for hematopoietic stem cells (HSC) from human (HemExTM;-TypeH) or mouse (HemExTM-Type9AØ). HemExTM series simply contain Insulin-Transferrin-Selenium-Ethanolamine (ITS-X) as supplement, which allows flexible combination of cytokines and small molecules, and enable expansion of HSCs.

* The products are supervised by Professor Satoshi Yamazaki in Division of Cell Regulation, Center for Experimental Medicine and Systems Biology, The Institute of Medical Science, The University of Tokyo.
*"HemEx" is a trademark of NIPRO CORPORATION.
* The products are for research use only. Not for therapeutic or diagnostic use.

製品外観

HemExTM-Type9AØ

Lineup

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Product name Product summary Product code
HemExTM-Type9AØ Serum-free, Mouse HSC expansion basal medium 87-590
HemExTM-TypeH Serum-free, Human HSC expansion basal medium 87-079

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HemExTM-Type9AØ

Specification

  • This medium is new improved version for HemExTM-Type9A.
  • This medium allows expansion of HSCs from single bone marrow-derived (BM) HSC by using newly developed supplement with high molecular weight compounds instead of PVA which was used in the previous version, HemExTM-Type9A.
  • This medium can be combined with genome editing tools to allow transplantation using genetically engineered homogeneous populations of cells.

* This medium requires the addition of cytokines for culturing HSC.

「HemEx-Type9AØ」フライヤー

Brochure of HemExTM-Type9AØ
Click the image to download the PDF file.


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HemExTM-TypeH

Specification

  • This product which is an albumin-free basal medium enables long-term growth of hematopoietic stem cells which has been difficult to cultivate.
  • This product allows to cultivate the cells reducing lot-to-lot variability and enables large-scale culturing of human hematopoietic stem cells maintaining undifferentiated condition. This is realized by adding newly developed high molecular weight pharmaceutical additives and substances* that show biological functions binding to receptors of cytokines etc.

* The required additives are Butyzamide, 740Y-P (CAS No. 236188-16-1), UM729, Flt3L (Peprotech #AF-300-19) .
L-Glutamine (Final concentration: 4 mM) should also be supplemented.
Please refer to the datasheet which can be downloaded at Product Information on this webpage.

「HemEx-TypeH」フライヤー

Brochure of HemExTM-TypeH
Click the image to download the PDF file.


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Reference

  1. Becker, HJ. et al., Cell Stem Cell., 30 (7), 987-1000 (2023). [DOI:10.1016/j.stem.2023.06.002]
  2. Wilkinson, AC. et al., Nat Protoc., 15 (2), 628-648 (2020). [DOI:10.1038/s41596-019-0263-2]
  3. Sakurai M. et al., Nature., 615 (7950), 127-133 (2023). [DOI:10.1038/s41586-023-05739-9]
  4. Sakurai M. et al., Protocol Exchange., (2023). [DOI:10.21203/rs.3.pex-2163/v1]
  5. Ishitsuka K. et al., Exp Hematol., 131:104138 (2024). [DOI:10.1016/j.exphem.2023.104138]

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Product Information

HemExTM-Type9AØ

[Date : June 24 2025 00:07]

Detail Product Name Product Code Supplier Size Price
HemEx-Type9A/O (#A5P10P01C)
DatasheetThis may not be the latest data sheet.
87-590 KSKCell Science & Technology Institute, Inc. 100 ml $194

Description Basal medium capable of proliferating mouse hematopoietic stem cells (HSC) while maintaining an undifferentiated state. Animal-free
Storage 4°C,Dark Strage CAS
Link

[Date : June 24 2025 00:07]

HemEx-Type9A/O (#A5P10P01C)


  • Product Code: 87-590
  • Supplier: KSK
  • Size: 100ml
  • Price: $194

Description Basal medium capable of proliferating mouse hematopoietic stem cells (HSC) while maintaining an undifferentiated state. Animal-free
Storage 4°C,Dark Strage CAS
Link


HemExTM-TypeH

[Date : June 24 2025 00:07]

Detail Product Name Product Code Supplier Size Price
HemEx-TypeH (#A2D10P01C)
DatasheetThis may not be the latest data sheet.
87-079 KSKCell Science & Technology Institute, Inc. 100 ml $272

Description Basal medium capable of proliferating human hematopoietic stem cells (hHSCs) while maintaining an undifferentiated state. Animal-free
Storage 4°C,Dark Strage CAS
Link

L-Glutamine Solution, 200mM (#1104)
DatasheetThis may not be the latest data sheet.
87-885 KSKCell Science & Technology Institute, Inc. 100 ml $39

Description This product is a quality certified L-Glutamine resolved in ultrapure water for serum-free culture, and filter sterilized by 0.2um pore size sterile filter. Cell culturing test and endotoxin test are completed. Concentration: 200 mM in ultrapure water (29.23 g/L), Old code: 1104
Storage -20°C CAS
Link

[Date : June 24 2025 00:07]

HemEx-TypeH (#A2D10P01C)


  • Product Code: 87-079
  • Supplier: KSK
  • Size: 100ml
  • Price: $272

Description Basal medium capable of proliferating human hematopoietic stem cells (hHSCs) while maintaining an undifferentiated state. Animal-free
Storage 4°C,Dark Strage CAS
Link

L-Glutamine Solution, 200mM (#1104)


  • Product Code: 87-885
  • Supplier: KSK
  • Size: 100ml
  • Price: $39

Description This product is a quality certified L-Glutamine resolved in ultrapure water for serum-free culture, and filter sterilized by 0.2um pore size sterile filter. Cell culturing test and endotoxin test are completed. Concentration: 200 mM in ultrapure water (29.23 g/L), Old code: 1104
Storage -20°C CAS
Link


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