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Temperature-responsive Protein A regin for antibody elution Byzen Pro(R)

Date:September 08 2014Web Page No:46143

Nomadic Bioscience Co., Ltd.

Very mild and best condition for antibodies
Temparature-responsive Protein A resin "Byzen Pro®"

Byzen Pro(R) : Temperature-responsive Protein A regin for antibody elution

Byzen Pro(R) is an affinity chromatography resin in which a novel Protein A mutant is used as a ligand.
The product provides a new antibody purification scheme where antibody is eluted under neutral pH by changing temperature, avoiding risk of antibody aggregation and decreased activity at acidic condition.

Features

  • Antibody is eluted under neutral pH. Byzen Pro(R) needs no acidic conditions.
    No risk of aggregation and decreased activity of antibody.
  • Binding and elution are controlled by temperature.
    Antibody is bound below 10ºC and eluted under room temperature condition or lower than 40C.
  • A buffer of your choice can be used for elution.
    PBS buffer can be used for elution step. Buffer exchange is not necessary.
  • Good antibody specificity
    Byzen Pro® is as specific as the conventional Protein A, showing excellent impurity elimination capability.
  • A recovery ratio is 95% or more.

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Purification procedure in a closed column

  1. Attach a 5 mL loop before the column, and wash the column with 5 CV of H2O.
  2. Immerse the column and the loop into a 4°C water bath and then equilibrate the column with buffer.
  3. Wait for 5 min for temperature equilibration, and then inject a sample.
  4. After wash the column to remove impurities, stop the pump. Take the column and the loop out of the 4ºC water bath and immerse them into a 40ºC water bath.
  5. Wait for 5 min for temperature equilibration, and then start the pump to elute antibody.
Byzen Pro(R) : Temperature-responsive Protein A regin for antibody elution

* Purification procedure in open column (no jacket) is also available.

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Specificity

  • Human IgG1, IgG2 and IgG4
  • Rabbit IgG and Goat IgG

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Specification

  • Bead Structure : Cross-linked agarose
  • Mean Particle Size : 70 um
  • Max. Back Pressure : 0.3 Mpa,3bar
  • Flow Rate (Recommend) : 125 – 250 cm/h
  • pH Range (Recommend) : pH 6 – pH 8
  • Binding Temperature : 4C
  • Eluting Temperature : 37C – 40C
  • Dynamic Binding Capacity : 26 – 34 mg/ml (20 cm column)
  • Storage Condition : 20% ethanol at 4C
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      Example data

      Byzen Pro(R) : Temperature-responsive Protein A regin for antibody elution

      Human IgG was purified by using Byzen Pro. When CV was 18, temparature has been changed from 4°C to 40°C and collected under neutral pH condition.

      [Date : October 12 2019 00:21]

      Detail Product Name Product Code Supplier Size Price
      Byzen Pro
      2512N05 NOMNomadic Bioscience Co., Ltd. 5 ml $800

      Description Human antibody is purified from cell culture by using Protein A affinity chromatography. Conventional Protein A resin uses strongly acidic buffer to elute antibody, exposing the target antibody to risk of aggregation and decreased activity. In contrast, Byzen ProR - the temperature-responding Protein A, allows antibody to be eluted under neutral pH by simply changing temperature. This eliminates any risk of aggregation and decreased activity of antibody, improving manufacturing efficiency of antibody drugs.
      Storage 4°C CAS
      Link

      Byzen Pro
      2512N25 NOMNomadic Bioscience Co., Ltd. 25 ml $2,700

      Description Human antibody is purified from cell culture by using Protein A affinity chromatography. Conventional Protein A resin uses strongly acidic buffer to elute antibody, exposing the target antibody to risk of aggregation and decreased activity. In contrast, Byzen ProR - the temperature-responding Protein A, allows antibody to be eluted under neutral pH by simply changing temperature. This eliminates any risk of aggregation and decreased activity of antibody, improving manufacturing efficiency of antibody drugs.
      Storage 4°C CAS
      Link

      [Date : October 12 2019 00:21]

      Byzen Pro


      • Product Code: 2512N05
      • Supplier: NOM
      • Size: 5ml
      • Price: $800

      Description Human antibody is purified from cell culture by using Protein A affinity chromatography. Conventional Protein A resin uses strongly acidic buffer to elute antibody, exposing the target antibody to risk of aggregation and decreased activity. In contrast, Byzen ProR - the temperature-responding Protein A, allows antibody to be eluted under neutral pH by simply changing temperature. This eliminates any risk of aggregation and decreased activity of antibody, improving manufacturing efficiency of antibody drugs.
      Storage 4°C CAS
      Link

      Byzen Pro


      • Product Code: 2512N25
      • Supplier: NOM
      • Size: 25ml
      • Price: $2,700

      Description Human antibody is purified from cell culture by using Protein A affinity chromatography. Conventional Protein A resin uses strongly acidic buffer to elute antibody, exposing the target antibody to risk of aggregation and decreased activity. In contrast, Byzen ProR - the temperature-responding Protein A, allows antibody to be eluted under neutral pH by simply changing temperature. This eliminates any risk of aggregation and decreased activity of antibody, improving manufacturing efficiency of antibody drugs.
      Storage 4°C CAS
      Link

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CONTACT

export@funakoshi.co.jp

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