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Temperature-responsive Protein A regin for antibody elution Byzen Pro(R)

Date:September 08 2014Web Page No:46143

Nomadic Bioscience Co., Ltd.

Very mild and best condition for antibodies
Temparature-responsive Protein A resin "Byzen Pro®"

Byzen Pro(R) : Temperature-responsive Protein A regin for antibody elution

Byzen Pro(R) is an affinity chromatography resin in which a novel Protein A mutant is used as a ligand.
The product provides a new antibody purification scheme where antibody is eluted under neutral pH by changing temperature, avoiding risk of antibody aggregation and decreased activity at acidic condition.

Features

  • Antibody is eluted under neutral pH. Byzen Pro(R) needs no acidic conditions.
    No risk of aggregation and decreased activity of antibody.
  • Binding and elution are controlled by temperature.
    Antibody is bound below 10ºC and eluted under room temperature condition or lower than 40C.
  • A buffer of your choice can be used for elution.
    PBS buffer can be used for elution step. Buffer exchange is not necessary.
  • Good antibody specificity
    Byzen Pro® is as specific as the conventional Protein A, showing excellent impurity elimination capability.
  • A recovery ratio is 95% or more.

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Purification procedure in a closed column

  1. Attach a 5 mL loop before the column, and wash the column with 5 CV of H2O.
  2. Immerse the column and the loop into a 4°C water bath and then equilibrate the column with buffer.
  3. Wait for 5 min for temperature equilibration, and then inject a sample.
  4. After wash the column to remove impurities, stop the pump. Take the column and the loop out of the 4ºC water bath and immerse them into a 40ºC water bath.
  5. Wait for 5 min for temperature equilibration, and then start the pump to elute antibody.
Byzen Pro(R) : Temperature-responsive Protein A regin for antibody elution

* Purification procedure in open column (no jacket) is also available.

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Specificity

  • Human IgG1, IgG2 and IgG4
  • Rabbit IgG and Goat IgG

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Specification

  • Bead Structure : Cross-linked polyvinyl alcohol
  • Mean Particle Size : 70 um
  • Max. Back Pressure : 0.3 Mpa,3bar
  • Flow Rate (Recommend) : 125 – 250 cm/h
  • pH Range (Recommend) : pH 6 – pH 8
  • Binding Temperature : 4C
  • Eluting Temperature : 37C – 40C
  • Dynamic Binding Capacity : 26 – 34 mg/ml (20 cm column)
  • Storage Condition : 20% ethanol at 4C
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      Example data

      Byzen Pro(R) : Temperature-responsive Protein A regin for antibody elution

      Human IgG was purified by using Byzen Pro. When CV was 18, temparature has been changed from 4°C to 40°C and collected under neutral pH condition.

      [Date : August 14 2018 23:09]

      Detail Product Name Product Code Supplier Size Price

      [Date : August 14 2018 23:09]

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CONTACT

export@funakoshi.co.jp

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