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Simple miRNA detection kit

Date:February 03 2016Web Page No:46135

Getting best microRNA (miRNA) is burdensome work? We can help you!
~ Bio Dynamics Laboratory's "Simple miRNA detection kit" ~

The "Simple miRNA Detection Kit" is a product for a simple and rapid detection of miRNA. Proprietary primers for targeting miRNA are NOT necessary, which provides versatile assay of miRNA of interest.

*This product has been featured in Nature Market Place (1)(2).

Features

  • Simple steps and rapid procedure
  • Detect target miRNA and small RNAs by RT-PCR with your own primer.
  • Proprietary "ON tag" can ligate to miRNA, also piRNA and 2'-O-methylated 3' terminal nucleotide.
  • To detect miRNA from total RNA, enrichment of miRNA is not necessary.
  • The size of the target miRNA can be determined.

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Steps

Note: miRNA specific primer is NOT included in the kit.

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Examples

Fig. 1. Detection of miRNA in human tissue total RNA Obtained PCR products with the Simple miRNA Detection Kit were subject to 10polyacrylamide gel electrophoresis. Target microRNAs, a) hsa-miR-16-5p, b) hsa-miR-24-3p, c) hsa-miR-30c-5p, d) hsa-miR-93-5p were detected in human heart, brain and spleen total RNA.
M: DynaMarker DNA Small

Fig. 2. Detection of miRNA in serially diluted HL-60 total RNA MicroRNA, hsa-miR-223-3p, was examined from 1) 50 ng, 2) 20 ng, 3) 10 ng , 4) 5 ng, 5) 2 ng, 6) 1 ng, 7) 0.5 ng, 8) 0.2 ng,, 9) 0.1 ng, 10) 0.05 ng, 11) 0.02 ng, 12) 0 ng of total RNA with the Simple miRNA Detection Kit. PCR products were subject to 10polyacrylamide gel electrophoresis.
M: DynaMarker DNA Small

For more information : please click here.

[Date : August 18 2018 00:13]

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[Date : August 18 2018 00:13]

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CONTACT

export@funakoshi.co.jp

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