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DMSO-free Cell freezing (cryopreserving) medium StemCell Keep and Cryoscarless DMSO-Free

Date：June 12 2020Web Page No：46011

BMG Inc.

Let’s preserve your cells “no DMSO and Serum“ condition!

Cryopreserving medium without DMSO and Serum : StemCell Keep
CryoScarless-Appearance

[ For cryopreservation of Stem Cells = the "StemCell Keep" ]
[ For cryopreservation of Various Cells = the "Cryoscarless DMSO-Free" ]

Background

Existent problem of preservation media contains DMSO
Very old method of cryopreservation was “Slow Programmed Freezing” using by 10% DMSO contained solution. However, big ice crystal is formed by this method and this crystal causes very poor cell viability (0.1-1%) for primate ES and iPS cells. Improved method is “vitrification” using by DAP213 (DMSO 2M, Acetamide 1M, Propylene glycol 3M).

What is vitrification?
Vitrification is the new method for preventing ice crystal formation by rapid freezing (＜15 sec) in LN2. However, this method requires rapid thawing to prevent recrystallization and experienced operators. It is known that DMSO has influence on OCT-4 expression and differentiation, Acetamide is identified as carcinogens. Moreover, DAP213 is high osmolarity solution – it means this solution has high toxicity. Therefore, development of low Toxicity, easy to use vitrification solution had been expected. StemCell Keep is the solution that overcomes these disadvantages of DAP213!

Solution freezed by Liquid Nitrogen is heated by 50℃/min.

Solution freezed by Liquid Nitrogen is heated by 50℃/min.
StemCell Keep does not have recrystallization during temperature rising.


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StemCell Keep Features

[ For cryopreservation of Stem Cells = the "StemCell Keep" ]

  • Animal-derived Protein-free, Serum-free, Xeno-free and DMSO-free
  • No risk of differentiation effect by DMSO
  • Maintain ES and iPS cells with colony formation by vitrification.
  • Maintain stem cell pluripotency after thawing.
  • High cytoprotection effect and vitrification technology by new original cryoprotectant material is allowed to cryopreserve ES and iPS cell colonies.
  • Sufficient for 100 vials.
  • Free of bacteria, fungi and mycoplasma contamination.
  • Long shelf life. Stable for 2 years at 4℃ after the date of manufacture.
  • Free Trial Sample is available! (Please contact your local distributor)
StemCell Keep and Cryoscarless DMSO-Free : StemCell Keep

Cryopreservation of human iPS cells

Data shows the attached colony and recovery rates of human iPS cells after thawing. Human iPS cells were frozen and stored in a existing vitrification solution (DAP213) and StemCell Keep for 1 week. The colony formation rate on the day after thawing and the cell number (recovery rate) after 4 days are shown as a percentage (%) compared with a non-frozen group. The frozen cells with StemCell Keep clearly showed a higher protective effect.



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Video

Outline



=>Download here!(mp4 file, approximately 10.7 MB)

Handling


=>Download here!(mp4 file, approximately 30.3 MB)


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Cryoscarless Features

[ For cryopreservation of Various Cells = the "Cryoscarless DMSO-Free" ]

  • Animal derived protein-free, Serum-free, Xeno-free and DMSO-free
  • High cell viability, and no risk of both cytotoxicity of DMSO and contamination by serum-derived proteins.
  • Consistent and high cell viability after thawing (> 90% for most of the cell lines).
  • Maintaining stem cell pluripotency after thawing.
  • Free of bacteria, fungi and mycoplasma contamination.
  • Long shelf life. Stable for 2 years at 4°C after the date of manufacture.
  • Free Trial Sample is available! (Please contact your local distributor)
Cryopreserving medium without DMSO and Serum : Cryoscarless DMSO-Free

Differentiation of rat mesenchymal stem cells

Rat mesenchymal stem cells were frozen and stored for 1 week in Cryoscarless. The viability was measured after thawing and after 6 h, and compared with that of cells frozen in 10% DMSO. The survival rates immediately after thawing (0 h) and after adhesion (6 h) were also higher when frozen in this product. Also, the cells frozen with Cryoscarless showed better retention of both differentiation potentials.



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FAQ

Common

Q-1. Is Certificate of Analysis available?

A-1. Yes.

Q-2. Are the products GMP grade?

A-2. No. However, products are manufactured in GMP-compliant facility.

Q-3. Are the products animal-free?

A-3. Yes. Both CryoScarless DMSO-Free and StemCell Keep are animal-free, xeno-free and chemically defined.

Q-4. Is phenol red contained in the product?

A-4. Both CryoScarless DMSO-free and StemCell Keep do not contain phenol red.

CryoScarless DMSO-Free

Q-1. What is the composition of CryoScarless DMSO-free?

A-1. Composition is not disclosed. However, as an effective ingredient, carboxylated poly-L-lysine (cPLL) is contained. CryoScarless DMSO-free is also protein-free and animal free.



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Product Information

StemCell Keep

StemCell Keep flyer Flyer PDF download

[Date : September 19 2024 00:08]

Detail Product Name Product Code Supplier Size Price
StemCell Keep
DatasheetThis may not be the latest data sheet. 		VPL-A1 BVDBMG Inc. 20 ml $166

Description StemCell Keep is a highly efficient cryopreserving solution (cell freezing medium) by vitrification for primate ES and iPS cells. Features 1. Animal-derived Protein-free and DMSO-free formulation. No risk of differentiation effect by DMSO. 2. Maintain ES and iPS cells with colony formation by vitrification. 3. Maintain stem cell pluripotency after thawing. 4. High cytoprotection effect and vitrification technology by new original cryoprotectant material is allowed to cryopreserve ES and iPS cell colonies.
Storage 4°C CAS
Link

[Date : September 19 2024 00:08]

StemCell Keep

DatasheetThis may not be the latest data sheet.


  • Product Code: VPL-A1
  • Supplier: BVD
  • Size: 20ml
  • Price: $166

Description StemCell Keep is a highly efficient cryopreserving solution (cell freezing medium) by vitrification for primate ES and iPS cells. Features 1. Animal-derived Protein-free and DMSO-free formulation. No risk of differentiation effect by DMSO. 2. Maintain ES and iPS cells with colony formation by vitrification. 3. Maintain stem cell pluripotency after thawing. 4. High cytoprotection effect and vitrification technology by new original cryoprotectant material is allowed to cryopreserve ES and iPS cell colonies.
Storage 4°C CAS
Link

CryoScarless DMSO-free

CryoScarless, DMSO-free flyer Flyer PDF download

[Date : September 19 2024 00:08]

Detail Product Name Product Code Supplier Size Price
CryoScarless DMSO-Free
DatasheetThis may not be the latest data sheet. 		CPL-A1 BVDBMG Inc. 100 ml $103

Description Citation : Stem Cell Research & Therapy20189:25 https://stemcellres.biomedcentral.com/articles/10.1186/s13287-017-0761-5 CryoScarless DMSO-Free is a serum-free and dimethyl sulfoxide (DMSO)-free cell freezing medium for long-term storage at -80℃ or in liquid nitrogen. Features 1. Serum-free and DMSO-free formulation. 2. High cell viability, and no risk of both cytotoxicity of DMSO and contamination by serum-derived proteins. 3. Consistent and high cell viability after thawing (> 90% for most of the cell lines).
Storage 4°C CAS
Link

[Date : September 19 2024 00:08]

CryoScarless DMSO-Free

DatasheetThis may not be the latest data sheet.


  • Product Code: CPL-A1
  • Supplier: BVD
  • Size: 100ml
  • Price: $103

Description Citation : Stem Cell Research & Therapy20189:25 https://stemcellres.biomedcentral.com/articles/10.1186/s13287-017-0761-5 CryoScarless DMSO-Free is a serum-free and dimethyl sulfoxide (DMSO)-free cell freezing medium for long-term storage at -80℃ or in liquid nitrogen. Features 1. Serum-free and DMSO-free formulation. 2. High cell viability, and no risk of both cytotoxicity of DMSO and contamination by serum-derived proteins. 3. Consistent and high cell viability after thawing (> 90% for most of the cell lines).
Storage 4°C CAS
Link




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