Cryopreserving (cell freezing) medium without DMSO and Serum StemCell Keep and Cryoscarless DMSO-Free
Date：November 18 2016Web Page No：46011
BioVerde, Inc.
Let’s preserve your cells “no DMSO and Serum “ condition!
[ For cryopreservation of Stem Cells = the "StemCell Keep" ]
*This product has been featured in Nature Market Place.
MEMO
Existent problem of preservation media contains DMSO and What is vitrification?
Very old method of cryopreservation was “Slow Programmed Freezing” using by 10% DMSO contained solution. However, big ice crystal is formed by this method and this crystal causes very poor cell viability (0.1-1%) for primate ES and iPS cells. Improved method is “vitrification” using by DAP213 (DMSO 2M, Acetamide 1M, Propylene glycol 3M).
Vitrification is the new method for preventing ice crystal formation by rapid freezing (＜15 sec) in LN2. However, this method requires rapid thawing to prevent recrystallization and experienced operators. It is known that DMSO has influence on OCT-4 expression and differentiation, Acetamide is identified as carcinogens. Moreover, DAP213 is high osmolarity solution – it means this solution has high toxicity. Therefore, development of low Toxicity, easy to use vitrification solution had been expected.StemCell Keep is the solution that overcomes these disadvantages of DAP213!
Solution freezed by Liquid Nitrogen is heated by 50℃/min.
StemCell Keep does not have recrystallization during temperature rising.
Features
- Animal-derived Protein-free, Serum-free and DMSO-free formulation. No risk of differentiation effect by DMSO.
- Xeno-freee.
- Maintain ES and iPS cells with colony formation by vitrification.
- Maintain stem cell pluripotency after thawing.
- High cytoprotection effect and vitrification technology by new original cryoprotectant material is allowed to cryopreserve ES and iPS cell colonies.
- Sufficient for 100 vials.
- Free of bacteria, fungi and mycoplasma contamination.
- Long shelf life. The product is stable for 2 years at 4C after the date of manufacture.
- Free Trial Sample is available! (Please contact your local distributor)
Outline Video
=>Download here!(mp4 file, approximately 10.7 MB)
Handling Video
=>Download here!(mp4 file, approximately 30.3 MB)
[ For cryopreservation of Various Cells = the "Cryoscarless DMSO-Free" ]
Features
- Animal derived protein-free, Serum-free and DMSO-free formulation.
- Xeno-free.
- High cell viability, and no risk of both cytotoxicity of DMSO and contamination by serum-derived proteins.
- Consistent and high cell viability after thawing (> 90% for most of the cell lines).
- Maintaining stem cell pluripotency after thawing.
- Free of bacteria, fungi and mycoplasma contamination.
- Long shelf life. The product is stable for 2 years at 4°C after the date of manufacture.
- Free Trial Sample is available! (Please contact your local distributor)
More data? Please download our brochure from hrere.
[Date : November 20 2018 23:11]
| Detail | Product Name | Product Code | Supplier | Size | Price | ||||||||||||||||||||||||||||||
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StemCell Keep Datasheet |
VPL-A1 | BVDBioVerde, Inc. | 20 ml | $200 | |||||||||||||||||||||||||||||||
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CryoScarless DMSO-Free Datasheet |
CPL-A1 | BVDBioVerde, Inc. | 100 ml | $125 | |||||||||||||||||||||||||||||||
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[Date : November 20 2018 23:11]
StemCell Keep
- Product Code: VPL-A1
- Supplier: BVD
- Size: 20ml
- Price: $200
| Description |
StemCell Keep is a highly efficient cryopreserving solution (cell freezing medium) by vitrification for primate ES and iPS cells. Features 1. Animal-derived Protein-free and DMSO-free formulation. No risk of differentiation effect by DMSO. 2. Maintain ES and iPS cells with colony formation by vitrification. 3. Maintain stem cell pluripotency after thawing. 4. High cytoprotection effect and vitrification technology by new original cryoprotectant material is allowed to cryopreserve ES and iPS cell colonies. |
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| Storage | 4°C | CAS | |
| Link | |||
CryoScarless DMSO-Free
- Product Code: CPL-A1
- Supplier: BVD
- Size: 100ml
- Price: $125
| Description |
Citation : Stem Cell Research & Therapy20189:25 https://stemcellres.biomedcentral.com/articles/10.1186/s13287-017-0761-5 CryoScarless DMSO-Free is a serum-free and dimethyl sulfoxide (DMSO)-free cell freezing medium for long-term storage at -80℃ or in liquid nitrogen. Features 1. Serum-free and DMSO-free formulation. 2. High cell viability, and no risk of both cytotoxicity of DMSO and contamination by serum-derived proteins. 3. Consistent and high cell viability after thawing (> 90% for most of the cell lines). |
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| Storage | 4°C | CAS | |
| Link | |||
CONTACT
export@funakoshi.co.jp
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