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HOME> Products> Protein / Enzyme> Other reagent> Actinase E

Enzyme that removes contaminating proteins by strong proteolytic action Actinase E

Date：May 23 2025Web Page No：160850

Kaken Pharmaceutical Co.,Ltd.

Actinase E is a high-purity enzyme that removes contaminating proteins by strong proteolytic action.

What is Actinase E
Features
Product Information

What is Actinase E

Actinase E is a proteolytic enzyme product derived from the culture filtrate of the actinomycete Streptomyces griseus. It was developed in Japan. Today, it is widely used in research around the world to remove protein/peptide components from samples such as sugars and nucleic acids.

Literature on Actinase E

Nomoto M. et al., J. Biochem (Tokyo), 48 (4), 593～602 (1960).
Schmid E. P. et al., J. Biol. Chem., 289 (12), 8194～8202 (2014).
Higashi K. et al., Carbohyd. Polym., 134, 557～565 (2015).

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Features

Low amino acid specificity and cleaves peptide bonds almost indiscriminately
High enzyme activity in the neutral range (pH7-9)
Unlike acid hydrolysis, it does not degrade tryptophan/methionine, allowing protein degradation even under mild reaction conditions.
The enzyme itself is inactivated by increasing temperature, however it can be reacted at high temperatures (50-55°C) in the presence of substrate.
Strong proteolytic activity (1,000 Tyrosine PU*/mg), and the degree of degradation reaches 75% to 90%.
＊PU: Enzyme activity that releases 1 μg of tyrosine per minute using casein as substrate

※Heat, acid, alkali, serum, potato and legume extracts, and the presence of rich salts cause a decrease in activity. There is no problem using normal saline (0.9% NaCl).

※Because the enzyme is calcium-dependent, the enzyme is rapidly deactivated when calcium ions are lost by treatment of the reaction mixture with EDTA, dialysis, ammonium sulfate, or ion exchange resin.

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Product Information

[Date : June 05 2025 00:07]

Detail Product Name Product Code Supplier Size Price

Actinase E KA-002 KNFKaken Pharmaceutical Co.,Ltd. 1 g $65

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Description	Actinase E, Enzyme unit (Tyrosine PU/g): 1,000,000, Origin: Streptomyces griseus
Storage		CAS
Link

Actinase E KA-001 KNFKaken Pharmaceutical Co.,Ltd. 5 g $169

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Description	Actinase E, Enzyme unit (Tyrosine PU/g): 1,000,000, Origin: Streptomyces griseus
Storage	4°C	CAS
Link	Natural compound Product List

[Date : June 05 2025 00:07]

Actinase E

Product Code: KA-002
Supplier: KNF
Size: 1g
Price: $65

Description	Actinase E, Enzyme unit (Tyrosine PU/g): 1,000,000, Origin: Streptomyces griseus
Storage		CAS
Link

Contact

Actinase E

Product Code: KA-001
Supplier: KNF
Size: 5g
Price: $169

Description	Actinase E, Enzyme unit (Tyrosine PU/g): 1,000,000, Origin: Streptomyces griseus
Storage	4°C	CAS
Link	Natural compound Product List

Contact

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CONTACT

export@funakoshi.co.jp

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