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HOME> Products> Molecular Biology> RNA MW marker> ^DynaMarkerDIG Labeled Blue Color Marker for Small RNA

DIG-labeled Prestain RNA Size Marker ^DynaMarkerDIG Labeled Blue Color Marker for Small RNA

Date：December 23 2025Web Page No：160840

<sup>DynaMarker <sup>Colored DIG Marker for Small RNA — Electrophoresis using 12.5% acrylamide - 7.5 M urea denaturing gel

Each band of RNA ladder marker is labeled with DIG (digoxigenin) together with blue dye.
In addition to confirming transfer efficiency to a membrane, it can also be used as a molecular weight marker in Northern hybridization using DIG-modified probes.

Background
Festures
Example of Use
Product Information

Background

To confirm the molecular weight during Northern hybridization under non-RI conditions, the gel portion containing the marker lane was cut off and stained with EtBr, or after transfer to a membrane, stained with methylene blue or other dyes¹. Since this product is DIG labeled, it can be detected using DIG antibody. In addition, since it is modified with visible light dye, it can be used for monitoring electrophoresis and checking transfer efficiency to membrane.

Reference
1. "Molecular Cloning: A Laboratory Manual, 3rd ed.",: Chapter 7.36, Cold Spring Harbor Laboratory Press (2001).

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Festures

It is very useful for Northern hybridization of small RNA such as miRNA.
It is ideal for electrophoresis and monitoring transfer efficiency to membranes (Fig. 1).
DIG is introduced into the colored nucleic acid that constitutes the marker. It can be detected by using alkaline phosphatase-modified DIG antibody and chemiluminescent substrate (Fig. 2).
High concordance rate was achieved with ^{Dyna Marker}Small RNA II (#DM192), which is a molecular weight marker for electrophoresis of small RNA.
It can be applied directly without heat denaturation.

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Example of Use

Left: Electrophoresis using 12.5% acrylamide - 7.5 M urea denaturing gel
Lane 1: This product (5 μL)
Lane 2: Human Breast total RNA (5 μg)
Lane 3: Breast Adenocarcinoma total RNA (5 μg)

Right: Blotted nylon membrane

Detection of various miRNAs from total RNA by Northern hybridization using DIG-labeled DNA probes

Antibody: Alkaline phosphatase-labeled DIG antibody
Substrate: CDP-Star^®^＊

＊CDP-Star is a registered trademark of Tropix Inc.

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Product Information

[Date : December 26 2025 00:09]

Detail Product Name Product Code Supplier Size Price

DynaMarker, DIG Labeled Blue Color Marker for Small RNA
DatasheetThis may not be the latest data sheet. DM270 BDLBioDynamics Laboratory Inc 125 µl $290

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Description	This product is blue prestained and DIG labeled RNA molecular ladder marker. It is suitable for monitoring electrophoresis and membrane transfer efficiency, and denaturing polyacrylamide immunodetection with anti-DIG antibody as non-radioactive Northern Blotting. It is supplied in a ready-to-use mixture and doesn’t require heating or addition of a denaturing agent before use.
Storage	-20°C	CAS
Link

[Date : December 26 2025 00:09]

DynaMarker, DIG Labeled Blue Color Marker for Small RNA

DatasheetThis may not be the latest data sheet.

Product Code: DM270
Supplier: BDL
Size: 125µl
Price: $290

Description	This product is blue prestained and DIG labeled RNA molecular ladder marker. It is suitable for monitoring electrophoresis and membrane transfer efficiency, and denaturing polyacrylamide immunodetection with anti-DIG antibody as non-radioactive Northern Blotting. It is supplied in a ready-to-use mixture and doesn’t require heating or addition of a denaturing agent before use.
Storage	-20°C	CAS
Link

Contact

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CONTACT

export@funakoshi.co.jp

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※Please note that Product Information or Price may change without notice.

DIG-labeled Prestain RNA Size Marker DynaMarker DIG Labeled Blue Color Marker for Small RNA

Background

Festures

Example of Use

Product Information

CONTACT

DIG-labeled Prestain RNA Size Marker ^DynaMarkerDIG Labeled Blue Color Marker for Small RNA