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Antibodies Specifically Recognizes Acetylated or Phosphorylated p53 Protein Modified p53 Specific Antibodies

Date：April 28 2026Web Page No：160839

BioAcademia, Inc.

We introduce the p53 antibody, which is the most important cancer-related gene detected in more than half of human cancers, and related antibody products involved in repairing DNA damage and inducing apoptosis.

Background

p53 and Ser46 phosphorylated p53

The p53 protein is the product of the tumor suppressor gene p53. It is a 393 amino acid protein with a molecular weight of 53 kDa. The p53 protein consists of a transcriptional activation domain and MM2 binding domain in the N-terminal region, a core domain with DNA-binding ability in the middle, and a tetramer-forming domain in the C-terminal region.
p53 is the most important cancer-related gene, with mutations detected in more than half of human cancers. The p53 protein is inactive in the absence of stress, but is activated by various stresses, especially DNA damage, and is involved in cell cycle arrest, repair of DNA damage, and induction of apoptosis. The p53 protein is activated by phosphorylation of multiple phosphorylation sites in the N-terminal and C-terminal regions, and by acetylation of lysine in the C-terminal region.
Among the phosphorylation sites, Ser46 is a site that is phosphorylated when DNA damage is too large to be repaired, and is reported to activate transcription of p53AIP1, a p53 target gene, and induce apoptosis. It has also been suggested that HIPK2, known as a transcription-coupled regulator, may be involved in phosphorylation of Ser46.

E2F1 and MdmX

In response to DNA damage, E2F1 is phosphorylated at serine 364, stabilized, and translocated to the nucleus where it is activated as a transcription factor. E2F1 activates the transcription of p73, a homolog of p53, and induces apoptosis.
MdmX binds to the transcriptional activation domains of p53 and p73 and inhibits cell-cycle arrest and apoptosis that depend on them. Phosphorylation of MdmX at Ser367 in response to DNA damage increases its binding to 14-3-3 and translocates to the nucleus. There, it is degraded by Mdm2, which activates p53.

    Reference:
  1. Serpi, R., et al., Pharmacol. Toxicol., 92 (5), 242-245 (2003). [PMID: 12753412]
  2. Brazdova, M., et al., Nucleic Acids Res., 30 (22), 4966-4974 (2002). [PMID: 12434001]
  3. Shieh, S. Y., et al., Genes Dev., 14 (3), 289-300 (2000). [PMID: 10673501]
  4. Sakaguchi, K., et al., Genes Dev., 12 (18), 2831-2841 (1998). [PMID: 9744860]


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Example of Use

p53 (p-Ser46) antibody, #71-115

Identification of Ser46-phosphorylated p53 protein by WB

Ser46 phosphorylated p53 protein was detected by Western blotting with p53 (pSer46) antibody (#71-115) (upper left picture) and another companies' product (upper right picture).
This product (#71-115) detected only the Ser46 phosphorylated p53 protein in response to DNA damage.

Lane1: Unstimulated MOLT-4 cell extracts
Lane2: MOLT-4 cell extracts treated with adriamycin (2 μg/mL) for 24 hours

    Cited by:
  • Taira, N., et al., Mol. Cell, 25, 725-738 (2007). [PMID: 17349958]

p53 (p-Ser315) antibody, #71-117

p53 (p-Ser315) antibody-WB

Ser315 phosphorylated p53 protein was detected by Western blotting using p53 (pSer315) antibody (#71-117). The lower panel is a control in which the total amount of p53 protein was detected.

Lane1: Untreated MCF7 cell extracts
Lane2: MCF7 cell extracts treated with nocodazole (100 ng/mL) for 48 hours


p53 (AcLys120) antibody, #71-131

Immunoprecipitation was performed with p53 antibody and detected by western blotting with p53 (AcLys120) antibody (#71-131) (middle picture). The lower panel shown a control in which the total amount of p53 was detected.

Lane1: Untreated HCT116 cell extracts
Lane2: HCT116 cell extracts in which the expression of the protein interacting with Tip60* was knocked down by siRNA

* Tip60: MYST family acetyltransferase that acetylates Lys120 of P53


MdmX (pSer367) antibody, #71-141

Induction of S367 phosphorylation after DNA damage is associated with increased binding of 14-3-3 to MdmX and accelerated MdmX degradation

Phosphorylation of MdmX at Ser367 occurs in response to DNA damage, binds to 14-3-3, and translocates to the nucleus to promote degradation

MCF cells were preincubated with proteasome inhibitor MG132, and DNA-damaging agents adriamycin or etoposide were added. After incubation for the indicated time periods, samples were taken, and crude extracts were prepared. Immunoprecipitation was performed with anti-MdmX antibody, and Western blotting was performed with anti-MdmX (pSer367) antibody (#71-141) and other antibodies.

    Cited by:
  • Okamoto, K., et al., Mol. Cell. Biol., 25, 9608-9620 (2005). [PMID: 16227609]


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Product Information

[Date : April 29 2026 00:09]

Detail Product Name Product Code Supplier Size Price
Anti-p53(pSer20), Phosphorylated, Mouse-Mono(17B6)
DatasheetThis may not be the latest data sheet. 		71-113 BAKBioAcademia, Inc. 100 µg $290

Description Concentration: 1.0 mg/ml, Buffer: PBS with 50% glycerol, Purity: Purified IgG fraction with protein A from hybridoma cell culture medium., Immunogen: Synthetic peptide containing phosph-Ser20, Isotype: Mouse IgG1κ, Reactivity: Human p53 phosphorylated at Ser20, p53 mutants are found in more than half of human cancers and are considered as the most important human cancer related gene. p53 is detected at 53kD position by electrophoresis and is composed of 393 amino acids. In the unstressed normal cells, the p53 level is low and it is inactive. However, with stress, especially with DNA damage, it is activated to promote arrest of cell cycle and repair of DNA damage, or induction of apoptosis. The functions of p53 are regulated by phosphorylation of serine and threonine, and acetylation of lysine at various sites in the molecule.
Storage -20°C
Antigen Species Host Species Mouse Class Label
Cross Reactivity Application ELISA,Western Blot
Clonality Format Purification Absorption
Link

Cancer-Related Antibody

Anti-p53(pSer46),Phosphorylated,Mouse-Mono(36)
DatasheetThis may not be the latest data sheet. 		71-115 BAKBioAcademia, Inc. 100 µg $290

Description Concentration: 1.0 mg/ml, Buffer: PBS with 50% glycerol, Purity: Purified IgG fraction with protein A from hybridoma cell culture medium., Immunogen: Synthetic peptide containing phospho-Ser46 of p53, Isotype: Mouse IgG1κ, Reactivity: Human p53-phosphorylated at Ser46., p53 mutants are found in more than half of human cancers and are considered as the most important human cancer related gene. p53 is detected at 53kD position by electrophoresis and is composed of 393 amino acids. In the unstressed normal cells the p53 level is low and it is inactive. However, with stress, especially with DNA damage, it is activated to promote arrest of cell cycle and repair of DNA damage, or induction of apoptosis. The functions of p53 are regulated by phosphorylation of serine and threonine, and acetylation of lysine at various sites in the molecule. Among the phosphorylation sites, Ser46 is phosphorylated when DNA damage is so severe as to become unrepairable, which leads to apoptosis by activating transcription of proapoptotic genes such as p53AIPI.As to the kinase of phosphorylation of Ser46, involvement of DYRK2 and ATM have been implicated.
Storage -20°C
Antigen Species Host Species Mouse Class Label
Cross Reactivity Application ELISA,IHC,Western Blot
Clonality Format Purification Absorption
Link

Cancer-Related Antibody

Anti-p53(pSer315), Phosphorylated, Mouse-Mono(18)
DatasheetThis may not be the latest data sheet. 		71-117 BAKBioAcademia, Inc. 100 µg $290

Description Concentration: 1.0 mg/ml, Buffer: PBS with 50% glycerol, Purity: Purified IgG fraction with protein A from hybridoma cell culture medium, Immunogen: Synthetic peptide containing phosphor-Ser315 of p53, Isotype: Mouse IgG2bκ, Reactivity: Human, p53 mutants are found in more than half of human cancers and are considered as the most important human cancer related gene. p53 is detected at 53kD position by electrophoresis and is composed of 393 amino acids. In the unstressed normal cells, the p53 level is low and it is inactive. However, with stress, especially with DNA damage, it is activated to promote arrest of cell cycle and repair of DNA damage, or induction of apoptosis. The functions and stability of p53 are regulated by phosphorylation of serine and threonine, and acetylation of lysine at various sites in the molecule. Ser315 is phosphorylated by aurora kinase and cycline-dependent kinases when cells are subjected to stress such as DNA damage and microtubule disruption by nocodazole. However the effect of the phosphorylation on the function of p53 is mostly unknown. This product is the purified IgG fraction obtained from serum-free culture medium of mouse hybridoma (clone #18) which produces monoclonal antibody that specifically recognizes human p53 protein with phospholyrated Ser315.
Storage -20°C
Antigen Species Host Species Mouse Class Label
Cross Reactivity Application ELISA,IHC,Western Blot
Clonality Format Purification Absorption
Link

Cancer-Related Antibody

Anti-p53(AcLys120),Acetylated,Mouse-Mono(10E5) <Anti-p53Acetyl-K120>
DatasheetThis may not be the latest data sheet. 		71-131 BAKBioAcademia, Inc. 100 µg $290

Description Concentration: 1.0 mg/ml, Buffer: PBS with 50% glycerol, Purity: Purified IgG fraction with protein A from hybridoma cell culture medium., Immunogen: Synthetic peptide containing acetyl-Lys315 of human p53, Isotype: Mouse IgG1κ, Reactivity: Human, p53 mutants are found in more than half of human cancers and are considered as the most important human cancer related gene. p53 is detected at 53kD position by electrophoresis and is composed of 393 amino acids. In the unstressed normal cells, the p53 level is low and it is inactive. However, with stress, especially with DNA damage, it is activated to promote arrest of cell cycle and repair of DNA damage, or induction of apoptosis. The functions and stability of p53 are regulated by phosphorylation of serine and threonine, and acetylation of lysine at various sites in the molecule. Acetylation of lysine 120 (acetyl-K120) of p53 occurs rapidly after DNA damage and is catalyzed by the MYST family acetyltransferases hMOF and TIP60, and activates transcription of proapoptotic genes, BAX and PUMA.
Storage -20°C
Antigen Species Host Species Mouse Class Label
Cross Reactivity Application ELISA,FCM,IF,IP,Western Blot
Clonality Format Purification Absorption
Link

Cancer-Related Antibody

Anti-p53 acetyl-K382, Mouse-Mono(2B7E4)
DatasheetThis may not be the latest data sheet. 		71-133 BAKBioAcademia, Inc. 100 µg $290

Description Concentration: 1.0 mg/ml, Buffer: PBS with 50% glycerol, Purity: Purified IgG fraction with protein A from hybridoma cell culture medium., Immunogen: Synthetic peptide containing acetyl-Lys382 of human p53, Isotype: Mouse IgG1κ, Reactivity: Human, p53 mutants are found in more than half of human cancers and are considered as the most important human cancer related gene. p53 is detected at 53kD position by electrophoresis and is composed of 393 amino acids. In the unstressed normal cells, the p53 level is low and it is inactive. However, with stress, especially with DNA damage, it is activated to promote arrest of cell cycle and repair of DNA damage, or induction of apoptosis. The functions and stability of p53 are regulated by phosphorylation of serine and threonine, and acetylation of lysine at various sites in the molecule. Acetylation of lysine 382 (acetyl-K382) of p53 occurs after DNA damage and is catalyzed by the p300/CBP acetyltransferase, which stabilizes p53 protein.
Storage
Antigen Species Host Species Mouse Class Label
Cross Reactivity Application IF,Western Blot
Clonality Format Purification Absorption
Link

Cancer-Related Antibody

Anti-E2F1(pSer364), Phosphorylated, Mouse-Mono(#2)
DatasheetThis may not be the latest data sheet. 		71-151 BAKBioAcademia, Inc. 100 µg $290

Description Concentration: 1.0 mg/ml, Buffer: PBS with 50% glycerol, Purity: Purified IgG fraction with protein A from hybridoma cell culture medium., Immunogen: Synthetic peptide corresponding to a sequence of human E2F1 protein including and surrounding phospho-Ser364, Isotype: Mouse IgG2bκ, Reactivity: Human E2F1 protein phosphorylated at Ser364. Not tested with other species, E2F1 is a member of E2F group of proteins that share common structural and functional domains and plays a major role during the G1/S transition in the mammalian cell cycle as a transcriptional factor. E2F1 is regulated during cell cycle progression. It is phosphorylated at Ser364 by Chk2 kinase in response to DNA damage, stabilized, mobilized to nucleus and activated as a transcription factor. It induces apoptosis by activating transcription of the p53 homolog, p73. E2F1 protein consists of 437 amino acids with a molecular mass of 46 kDa.
Storage -20°C
Antigen Species Host Species Mouse Class Label
Cross Reactivity Application ELISA,Western Blot
Clonality Format Purification Absorption
Link

Cancer-Related Antibody

Anti-MdmX(pSer367), Phosphorylated, Mouse-Mono(#15) <Anti-Mdm4>
DatasheetThis may not be the latest data sheet. 		71-141 BAKBioAcademia, Inc. 100 µg $290

Description Concentration: 1.0 mg/ml, Buffer: PBS with 50% glycerol, Purity: Purified IgG fraction with protein A from hybridoma cell culture medium., Immunogen: Synthetic peptide corresponding to a sequence of human Mdmx protein surrounding phospho-Ser367, Isotype: Mouse IgG2bκ, Reactivity: Human and mouse MdmX proteins phosphorylated at Ser367, MdmX (synonyms: Mdm4, HdmX) inhibits p53-and p73-dependent cell cycle arrest and apoptosis by binding to the transcription activation domains of these proteins. MdmX consists of 490 amino acids with the molecular weight of 54,864 and contains a RIING-finger domain and a nuclear transport signal. It is known that the protein migrates aberrantly in SDS-PAGE at the position of an 80-kDa protein. MdmX is phosphorylated at Ser367 by Chk2 kinase downstream of ATM in response to DNA damage, and as a result, it binds to14-3-3 and is transported into nucleus where it is degraded by Mdm2. This process activates the p53 functions.
Storage -20°C
Antigen Species Host Species Mouse Class Label
Cross Reactivity Application ELISA,IP,Western Blot
Clonality Format Purification Absorption
Link

Cancer-Related Antibody

[Date : April 29 2026 00:09]

Anti-p53(pSer20), Phosphorylated, Mouse-Mono(17B6)

DatasheetThis may not be the latest data sheet.


  • Product Code: 71-113
  • Supplier: BAK
  • Size: 100µg
  • Price: $290

Description Concentration: 1.0 mg/ml, Buffer: PBS with 50% glycerol, Purity: Purified IgG fraction with protein A from hybridoma cell culture medium., Immunogen: Synthetic peptide containing phosph-Ser20, Isotype: Mouse IgG1κ, Reactivity: Human p53 phosphorylated at Ser20, p53 mutants are found in more than half of human cancers and are considered as the most important human cancer related gene. p53 is detected at 53kD position by electrophoresis and is composed of 393 amino acids. In the unstressed normal cells, the p53 level is low and it is inactive. However, with stress, especially with DNA damage, it is activated to promote arrest of cell cycle and repair of DNA damage, or induction of apoptosis. The functions of p53 are regulated by phosphorylation of serine and threonine, and acetylation of lysine at various sites in the molecule.
Storage -20°C
Antigen Species Host Species Mouse
Class Label
Cross Reactivity Application ELISA,Western Blot
Clonality Format
Purification Absorption
Link

Cancer-Related Antibody

Anti-p53(pSer46),Phosphorylated,Mouse-Mono(36)

DatasheetThis may not be the latest data sheet.


  • Product Code: 71-115
  • Supplier: BAK
  • Size: 100µg
  • Price: $290

Description Concentration: 1.0 mg/ml, Buffer: PBS with 50% glycerol, Purity: Purified IgG fraction with protein A from hybridoma cell culture medium., Immunogen: Synthetic peptide containing phospho-Ser46 of p53, Isotype: Mouse IgG1κ, Reactivity: Human p53-phosphorylated at Ser46., p53 mutants are found in more than half of human cancers and are considered as the most important human cancer related gene. p53 is detected at 53kD position by electrophoresis and is composed of 393 amino acids. In the unstressed normal cells the p53 level is low and it is inactive. However, with stress, especially with DNA damage, it is activated to promote arrest of cell cycle and repair of DNA damage, or induction of apoptosis. The functions of p53 are regulated by phosphorylation of serine and threonine, and acetylation of lysine at various sites in the molecule. Among the phosphorylation sites, Ser46 is phosphorylated when DNA damage is so severe as to become unrepairable, which leads to apoptosis by activating transcription of proapoptotic genes such as p53AIPI.As to the kinase of phosphorylation of Ser46, involvement of DYRK2 and ATM have been implicated.
Storage -20°C
Antigen Species Host Species Mouse
Class Label
Cross Reactivity Application ELISA,IHC,Western Blot
Clonality Format
Purification Absorption
Link

Cancer-Related Antibody

Anti-p53(pSer315), Phosphorylated, Mouse-Mono(18)

DatasheetThis may not be the latest data sheet.


  • Product Code: 71-117
  • Supplier: BAK
  • Size: 100µg
  • Price: $290

Description Concentration: 1.0 mg/ml, Buffer: PBS with 50% glycerol, Purity: Purified IgG fraction with protein A from hybridoma cell culture medium, Immunogen: Synthetic peptide containing phosphor-Ser315 of p53, Isotype: Mouse IgG2bκ, Reactivity: Human, p53 mutants are found in more than half of human cancers and are considered as the most important human cancer related gene. p53 is detected at 53kD position by electrophoresis and is composed of 393 amino acids. In the unstressed normal cells, the p53 level is low and it is inactive. However, with stress, especially with DNA damage, it is activated to promote arrest of cell cycle and repair of DNA damage, or induction of apoptosis. The functions and stability of p53 are regulated by phosphorylation of serine and threonine, and acetylation of lysine at various sites in the molecule. Ser315 is phosphorylated by aurora kinase and cycline-dependent kinases when cells are subjected to stress such as DNA damage and microtubule disruption by nocodazole. However the effect of the phosphorylation on the function of p53 is mostly unknown. This product is the purified IgG fraction obtained from serum-free culture medium of mouse hybridoma (clone #18) which produces monoclonal antibody that specifically recognizes human p53 protein with phospholyrated Ser315.
Storage -20°C
Antigen Species Host Species Mouse
Class Label
Cross Reactivity Application ELISA,IHC,Western Blot
Clonality Format
Purification Absorption
Link

Cancer-Related Antibody

Anti-p53(AcLys120),Acetylated,Mouse-Mono(10E5) <Anti-p53Acetyl-K120>

DatasheetThis may not be the latest data sheet.


  • Product Code: 71-131
  • Supplier: BAK
  • Size: 100µg
  • Price: $290

Description Concentration: 1.0 mg/ml, Buffer: PBS with 50% glycerol, Purity: Purified IgG fraction with protein A from hybridoma cell culture medium., Immunogen: Synthetic peptide containing acetyl-Lys315 of human p53, Isotype: Mouse IgG1κ, Reactivity: Human, p53 mutants are found in more than half of human cancers and are considered as the most important human cancer related gene. p53 is detected at 53kD position by electrophoresis and is composed of 393 amino acids. In the unstressed normal cells, the p53 level is low and it is inactive. However, with stress, especially with DNA damage, it is activated to promote arrest of cell cycle and repair of DNA damage, or induction of apoptosis. The functions and stability of p53 are regulated by phosphorylation of serine and threonine, and acetylation of lysine at various sites in the molecule. Acetylation of lysine 120 (acetyl-K120) of p53 occurs rapidly after DNA damage and is catalyzed by the MYST family acetyltransferases hMOF and TIP60, and activates transcription of proapoptotic genes, BAX and PUMA.
Storage -20°C
Antigen Species Host Species Mouse
Class Label
Cross Reactivity Application ELISA,FCM,IF,IP,Western Blot
Clonality Format
Purification Absorption
Link

Cancer-Related Antibody

Anti-p53 acetyl-K382, Mouse-Mono(2B7E4)

DatasheetThis may not be the latest data sheet.


  • Product Code: 71-133
  • Supplier: BAK
  • Size: 100µg
  • Price: $290

Description Concentration: 1.0 mg/ml, Buffer: PBS with 50% glycerol, Purity: Purified IgG fraction with protein A from hybridoma cell culture medium., Immunogen: Synthetic peptide containing acetyl-Lys382 of human p53, Isotype: Mouse IgG1κ, Reactivity: Human, p53 mutants are found in more than half of human cancers and are considered as the most important human cancer related gene. p53 is detected at 53kD position by electrophoresis and is composed of 393 amino acids. In the unstressed normal cells, the p53 level is low and it is inactive. However, with stress, especially with DNA damage, it is activated to promote arrest of cell cycle and repair of DNA damage, or induction of apoptosis. The functions and stability of p53 are regulated by phosphorylation of serine and threonine, and acetylation of lysine at various sites in the molecule. Acetylation of lysine 382 (acetyl-K382) of p53 occurs after DNA damage and is catalyzed by the p300/CBP acetyltransferase, which stabilizes p53 protein.
Storage
Antigen Species Host Species Mouse
Class Label
Cross Reactivity Application IF,Western Blot
Clonality Format
Purification Absorption
Link

Cancer-Related Antibody

Anti-E2F1(pSer364), Phosphorylated, Mouse-Mono(#2)

DatasheetThis may not be the latest data sheet.


  • Product Code: 71-151
  • Supplier: BAK
  • Size: 100µg
  • Price: $290

Description Concentration: 1.0 mg/ml, Buffer: PBS with 50% glycerol, Purity: Purified IgG fraction with protein A from hybridoma cell culture medium., Immunogen: Synthetic peptide corresponding to a sequence of human E2F1 protein including and surrounding phospho-Ser364, Isotype: Mouse IgG2bκ, Reactivity: Human E2F1 protein phosphorylated at Ser364. Not tested with other species, E2F1 is a member of E2F group of proteins that share common structural and functional domains and plays a major role during the G1/S transition in the mammalian cell cycle as a transcriptional factor. E2F1 is regulated during cell cycle progression. It is phosphorylated at Ser364 by Chk2 kinase in response to DNA damage, stabilized, mobilized to nucleus and activated as a transcription factor. It induces apoptosis by activating transcription of the p53 homolog, p73. E2F1 protein consists of 437 amino acids with a molecular mass of 46 kDa.
Storage -20°C
Antigen Species Host Species Mouse
Class Label
Cross Reactivity Application ELISA,Western Blot
Clonality Format
Purification Absorption
Link

Cancer-Related Antibody

Anti-MdmX(pSer367), Phosphorylated, Mouse-Mono(#15) <Anti-Mdm4>

DatasheetThis may not be the latest data sheet.


  • Product Code: 71-141
  • Supplier: BAK
  • Size: 100µg
  • Price: $290

Description Concentration: 1.0 mg/ml, Buffer: PBS with 50% glycerol, Purity: Purified IgG fraction with protein A from hybridoma cell culture medium., Immunogen: Synthetic peptide corresponding to a sequence of human Mdmx protein surrounding phospho-Ser367, Isotype: Mouse IgG2bκ, Reactivity: Human and mouse MdmX proteins phosphorylated at Ser367, MdmX (synonyms: Mdm4, HdmX) inhibits p53-and p73-dependent cell cycle arrest and apoptosis by binding to the transcription activation domains of these proteins. MdmX consists of 490 amino acids with the molecular weight of 54,864 and contains a RIING-finger domain and a nuclear transport signal. It is known that the protein migrates aberrantly in SDS-PAGE at the position of an 80-kDa protein. MdmX is phosphorylated at Ser367 by Chk2 kinase downstream of ATM in response to DNA damage, and as a result, it binds to14-3-3 and is transported into nucleus where it is degraded by Mdm2. This process activates the p53 functions.
Storage -20°C
Antigen Species Host Species Mouse
Class Label
Cross Reactivity Application ELISA,IP,Western Blot
Clonality Format
Purification Absorption
Link

Cancer-Related Antibody

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