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The de facto standard antibody for Drebrin research (M2F6) is now available with higher quality! Drebrin antibody (M2F6-Shirao®) Mouse mAb, clone M2F6

Date：January 20 2026Web Page No：160838

Drebrin is an actin-binding protein that regulates the intracellular stability of actin filaments and plays a role in a wide range of cellular functions mediated by cell movement and morphological changes.
Drebrin antibody (clone: M2F6) has been widely used for many years as the de facto standard in neuroscience, making significant contributions to research on neuronal differentiation, brain development, and synaptic plasticity. It is extremely useful as a neuronal maturation marker because it clearly stains Drebrin localized in dendritic spines. In recent years, it is utilized also for evaluating the maturity of neurons derived from human iPS cells, thereby strongly supporting advances in iPS cell research. In addition, it has become an important research tool for studies on Drebrin beyond the field of neuroscience. The newly launched “Drebrin antibody (M2F6-Shirao®) Mouse mAb, clone M2F6” is produced by recovering antibodies from the hybridoma culture supernatant of the original strain, resulting in a higher antibody titer compared with conventional products.

* This product is for research use only.

Cultured-neurons derived from rat hippocampus Super-resolution microscopy (STORM) observation (cultured neurons derived from rat hippocampus)

What is Drebrin?

Drebrin was originally reported in chicken brains (Shirao et al., 1984) and named for its developmentally regulated expression in the brain (Developmentally REgulated BRain proteIN). It is an actin-binding protein involved in neuronal cytoskeletal dynamics and is classified into two major isoforms, Drebrin E and Drebrin A, which are generated by alternative splicing of a single DBN1 gene.　 Both Drebrin A and Drebrin E share a common domain structure at their N-terminus. While the amino acid sequences of Drebrin A and Drebrin E are nearly identical, only Drebrin A contains an additional sequence known as Ins2. Drebrin E is primarily expressed during fetal and juvenile stages, whereas Drebrin A is specifically expressed in neurons at the mature stage.

Structure of human Drebrin

Figure. Structure of human Drebrin

Drebrin E accumulates extensively in axonal growth cone of neurons, affecting neurite outgrowth and axon growth. As neurons mature, Drebrin E disappears and Drebrin A comes to appear instead. Drebrin A accumulates at the heads of dendritic spines and its localization is known to change in response to NMDA receptor activity. This Drebrin dynamics plays a crucial role in synaptic plasticity, the fundamental mechanism underlying learning and memory. In Alzheimer’s disease associated with memory impairment, postmortem analyses of human brain tissue have shown that Drebrin levels are already reduced at the mild cognitive impairment (MCI) stage. In addition, in vitro studies have demonstrated an amyloid-β (Aβ) oligomer–dependent decrease in Drebrin.


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Features

  • This antibody is derived from clone M2F6, established by Shirao et al. who reported Drebrin first.
  • Drebrin antibody (clone: M2F6) has been used as the global de facto standard in Drebrin research.
  • It is useful as a marker for dendritic spines in mature neurons or as a marker for evaluating neuronal maturation.

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Specifications

  • Host species: Mouse
  • Clone name: M2F6
  • Immunogen: Purified Chicken Drebrin E *1
  • Reactivity: Human, Mouse, Rat, Chicken, Cat
  • Application: IC、IHC(frozen or paraffin sections), IP, WB *2
  • Isotype: IgG1

*1 This antibody recognizes both Drebrin A and Drebrin E isoforms.
*2 IC: Immunocytochemistry, IHC: Immunohistochemistry, IP: Immunoprecipitation, WB: Western blotting


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Verification of antibody specificity

The specificity of this antibody was verified by immunohistochemical staining (lower left panels) and Western blotting (lower right panel) using Drebrin knockout mice.
(Specimen: Mouse cerebral cortex, WT: Wild type mouse, DXKO: Drebrin knockout mouse)

Immunohistochemical staining of a frozen tissue section
Western blotting

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Application Examples

Cell staining: Staining of developing primary neurons from rat hippocampus

Staining of Drebrin in the Developmental Process of Cultured Neuronal Cells Derived from the Rat Hippocampus1

Localization of Drebrin in primary rat hippocampal neurons was observed.
Staining of developing neurons (Stage 1-4) with this antibody visualized Drebrin accumulation at the growth cones of neurite tips (Green: Drebrin, Red: F-actin).


Staining of Drebrin in the Developmental Process of Cultured Neuronal Cells Derived from Rat Hippocampus 2

At the maturation stage of neurons (day 21), Drebrin accumulation is observed in dendritic spine heads.
(Green: Drebrin, Red: MAP2, Blue: DAPI)


Cell staining: Staining of human iPS cell-derived neurons

Cell staining: Staining of human iPS cell-derived neurons

As seen in the case of primary cultured neurons, Drebrin accumulates at the growth cones of neurite tips in immature, early-stage (3 weeks post-culture) human iPS cell-derived neurons, similar to primary cultured neurons.
(Red: MAP2, Green: Drebrin, Blue: DAPI)

For mature human iPS cell-derived neurons, please refer to the following publication.
Reference: Lin, W., et al., iScience, 26(4):106285 (2023). [PMID: 37034988]


Immunohistochemical staining: Paraffin sections of human cerebral cortex

Staining images of paraffin sections of human cerebral cortex

Drebrin accumulation in spines (granular staining) are visible as granular staining. (Provided by Professor Yokoo, Gunma University)


Immunohistochemical staining: Expression of Drebrin in the Alzheimer's disease brain

Immunohistochemical staining: Expression of Drebrin in the Alzheimer's disease brain

Immunohistochemical staining of Human hippocampal tissue specimens from Alzheimer's disease patients was performed using this antibody.
Although expression of Synaptophysin, a presynaptic marker, was not significantly changed, expression of Drebrin was reduced in patient brains.


Western Blot: Expression of Drebrin in Alzheimer's Disease Brain

Western Blot: Expression of Drebrin in Alzheimer's Disease Brain

Western blot analysis revealed reduced expression of Drebrin in Alzheimer's disease patients, while synaptophysin, a presynaptic marker, did not show significant expression change.
Top panel: Drebrin, Bottom panel: Synaptophysin
Left two lanes: Control specimens, Right two lanes: Specimens from Alzheimer's disease patients


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Publications

  1. Zhao, M., et al., Neural Regen. Res., 19(7):1602-1607 (2024). [PMID: 38051905]
  2. Takahashi, K., et al., Sci. Rep., 14(1):11519 (2024). [PMID: 38769131]
  3. Yamazaki, H., et al., Neurosci. Res., 199:30-35 (2024). [PMID: 37659612]
  4. Kreis, P., et al., Nat. Commun., 10(1):486 (2019). [PMID: 30700723]
  5. Counts, S.E, et al., J. Neuropathol. Exp. Neurol., 65(6):592-601 (2006). [PMID: 16783169]
  6. Shirao, T. and Obata, K., Brain Res., 394(2):233-244 (1986). [PMID: 3768727]
  7. Shirao, T. and Sekino, Y., (Eds.) (2017) Drebrin. (Advances in Experimental Medicine and Biology, vol 1006) Tokyo, Springer

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Product Information

[Date : January 22 2026 00:07]

Detail Product Name Product Code Supplier Size Price
Drebrin antibody (M2F6-Shirao) Mouse mAb
DatasheetThis may not be the latest data sheet. 		AAB002 ALZ 100 µl $759

Description Old clone name: M2F6, Letter of Assurance (LoA) is required for purchase.
Storage -20°C
Antigen Species Chicken Host Species Mouse Class IgG Label Unlabeled
Cross Reactivity Human/Mouse/Rat/Cat/Chicken Application IC,IHC,IP,Western Blot
Clonality Format Purification Protein A/G Affinity Purified Absorption
Link

[Date : January 22 2026 00:07]

Drebrin antibody (M2F6-Shirao) Mouse mAb

DatasheetThis may not be the latest data sheet.


  • Product Code: AAB002
  • Supplier: ALZ
  • Size: 100µl
  • Price: $759

Description Old clone name: M2F6, Letter of Assurance (LoA) is required for purchase.
Storage -20°C
Antigen Species Chicken Host Species Mouse
Class IgG Label Unlabeled
Cross Reactivity Human/Mouse/Rat/Cat/Chicken Application IC,IHC,IP,Western Blot
Clonality Format
Purification Protein A/G Affinity Purified Absorption
Link


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CONTACT

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