How to search

HOME> Products> Assay Kits> Others> ONEPot Immunoassay Kit ＜OpenGUS Method＞
HOME> Products> Protein / Enzyme> Other reagent> ONEPot Immunoassay Kit ＜OpenGUS Method＞

Wash-Free Immunoassay Construction Kit ONEPot Immunoassay Kit ＜OpenGUS Method＞

Date：August 05 2026Web Page No：160804

BioDynamics Laboratory Inc

This kit is designed for constructing protein measurement systems. It operates on the principle of the OpenGUS Immunoassay using a mutant variant of β-glucuronidase (GUS). Compared to traditional ELISA, this method offers simpler operations and requires a smaller amount of antibodies. It also provides higher quantitativeness in less time than Western blotting.

＊The products are for research use only.

Features

  • No Immobilization or Washing Required: Eliminates the need to immobilize antibodies onto plates or wash the wells.
  • Sandwich Detection: Detects target antigens by sandwiching them between two types of antibodies (Mouse IgG1). For proteins that form stable multimers, detection may be possible using only a single antibody.
  • Simple Mixing: Antibodies and the detection enzyme bind via affinity simply by mixing them together.
  • Antibody Savings: Requires only approximately (7 μg) of each of the two antibodies per 96 wells.
  • Automation-Friendly: Highly compatible with automated systems and generates minimal liquid waste.
  • Flexible Options: Available as a fluorescent measurement kit (#DS850) or a colorimetric measurement kit (#DS860).

Go back to index

Comparison with Sandwich ELISA

ONEPot Kit Sandwich ELISA
Antibody Immobilization Not required Required (overnight or longer)
Well Washing Not required Required (2 to 3 times)
Reaction Time 2 hours 10 minutes Approx. 3 hours
(+1 day if including immobilization)
Antibody Amount
(per 96 wells)		 Approx. 7 μg×2 types Capture: Approx. 100μg
Detection: up to a few μg
Detection Antibody Unlabeled Labeling required
Antigen Type Any arbitrary antigen can be measured Any arbitrary antigen can be measured
Compatibility with Automation Excellent Good
Liquid Waste Volume
(per 96 wells)		 Approx. 30 mL Approx. 200 mL

Go back to index

Principle

ONEPot Kit Principle

E. coli β-glucuronidase (GUS) exhibits enzymatic activity by forming a tetramer. The mutant GUS used in this product is fused with an antibody-binding domain; it forms dimers naturally but rarely forms tetramers on its own.
When the target antigen is present, the antigen-antibody reactions link the mutant GUS dimers together, forcing the formation of an active tetramer. The resulting enzymatic activity produces a signal dependent on the antigen concentration.

Go back to index

Protocol Overview

  1. Mix the measurement sample (or purified antigen for the standard curve), the two types of antibodies, and the enzyme included in the kit.
  2. Incubate at room temperature for 60 minutes.
  3. Add the substrate included in the kit.
  4. Incubate at room temperature for 60 minutes.
  5. Measure the fluorescence or absorbance using a plate reader.

Go back to index

Application Example

Measurement of Lactoferrin Concentration in Saliva and Tears

Lactoferrin concentrations in saliva and tears were measured using this product and compared with the results obtained from a commercially available Lactoferrin ELISA kit.

Sample
Type		 Dilution Lactoferrin Conc.
(Calculated from Standard Curve)
Saliva 1,000-fold 6.2 μg/mL
2,000-fold 5.7 μg/mL
Tears 200,000-fold 2,158 μg/mL
200,000-fold 2,175 μg/mL

Results with a Commercial ELISA Kit

Sample
Type		 Dilution Lactoferrin Conc.
(Calculated from Standard Curve)
Saliva 300-fold 4.2 μg/mL
600-fold 4.4 μg/mL
Tears 40,000-fold 1,831 μg/mL
80,000-fold 1,734 μg/mL

Results with ONEPot Kit

Standard Curve with This Product (ONEPot Kit)
Standard Curve with ONEPot Kit
  • Comparable Results: The measured values obtained from the commercial ELISA kit and this product were highly comparable.
  • Single Antibody Detection: Only a single type of antibody was used for the measurement with this product. This successful detection is attributed to the multimeric nature of lactoferrin.
  • Plot Axis: The vertical axis of the standard curve (Absorbance) represents the [Signal − Background] value.
  • Antibody Used: Anti-Lactoferrin, Human, Mouse-Mono (1A1) (HyTest, #4L2-1A1).

Go back to index

Original Articles

  1. Su, J., et al., "Noncompetitive homogeneous immunodetection of small molecules based on beta-glucuronidase complementation.", Analyst, 143 (9), 2096-2101 (2018). [PMID:29634056]
  2. Su, J., et al., "Creation of stable and strictly regulated enzyme switch for signal-on immunodetection of various small antigens.", J. Biosci. Bioeng., 128 (6), 677-682 (2019). [PMID:31235413]
  3. Su, J., et al., "Transmembrane signaling on a protocell: Creation of receptor-enzyme chimeras for immunodetection of specific antibodies and antigens.", Sci. Rep., 9 (1), 18189 (2019). [PMID:31796769]
  4. Zhu, B., et al., "Creating a thermostable beta-glucuronidase switch for homogeneous immunoassay by disruption of conserved salt bridges at diagonal interfaces.", Biochemistry, 62 (2), 309-317 (2023). [PMID:35849118]
  5. Zhu, B., et al., "Customizable OpenGUS immunoassay: A homogeneous detection system using β-glucuronidase switch and label-free antibody.", Biosens. Bioelectron., 267, 116796 (2025). [PMID:39316870]

Go back to index

Kit Components (100 wells / kit)

  • Recombinant GUS mixture
  • Reaction buffer
  • Fluorescent substrate (Included only in #DS850)
  • Colorimetric substrate (Included only in #DS860)

Go back to index

Required Materials (Not Included)

  • A pair of antibodies (Mouse IgG1) capable of sandwiching the target antigen
  • Purified antigen (for generating the standard curve)
  • 96-well plate (compatible with the chosen detection method)
  • Fluorescence or absorbance microplate reader
    　　Fluorescence (#DS850): Excitation 340 nm / Emission 450 nm
    　　Colorimetric (#DS860): Main wavelength 405 nm (Correction wavelength 660 nm)

Go back to index

Precautions

  • Antigen Concentration: If the target antigen is a protein, it is recommended to use samples containing (3 μg/ml) or more.
  • Antibody Compatibility: Even if an antibody pair works successfully in a standard sandwich ELISA, it may not always be compatible with this kit.
  • Sample Composition: Components within the sample solution can significantly influence signal intensity.
  • Incompatible Samples: Samples containing high amounts of immunoglobulins, such as human serum, plasma, or whole blood, cannot be used due to high background interference.

Go back to index

Product Information

[Date : August 05 2026 00:08]

Detail Product Name Product Code Supplier Size Price
ONEPot Immunoassay Kit, Fluorescent <OpenGUS Method>
DatasheetThis may not be the latest data sheet. 		DS850 BDLBioDynamics Laboratory Inc 1 kit $480

Description Kit for constructing a protein assay system. Assay is based on the principle of OpenGUS Immunoassay using mutant β-glucuronidase (GUS). Two types of antibodies (Mouse IgG1) against the target antigen are prepared, and the assay sample and the enzyme supplied with the kit are mixed and incubated for detection. For fluorescence measurement.
Storage -80°C CAS
Link

ONEPot Immunoassay Kit, Colorimetric <OpenGUS Method>
DatasheetThis may not be the latest data sheet. 		DS860 BDLBioDynamics Laboratory Inc 1 kit $480

Description Kit for constructing a protein assay system. Assay is based on the principle of OpenGUS Immunoassay using mutant β-glucuronidase (GUS). Two types of antibodies (Mouse IgG1) against the target antigen are prepared, and the assay sample and the enzyme supplied with the kit are mixed and incubated for detection. For absorbance measurement.
Storage -80°C CAS
Link

[Date : August 05 2026 00:08]

ONEPot Immunoassay Kit, Fluorescent <OpenGUS Method>

DatasheetThis may not be the latest data sheet.


  • Product Code: DS850
  • Supplier: BDL
  • Size: 1kit
  • Price: $480

Description Kit for constructing a protein assay system. Assay is based on the principle of OpenGUS Immunoassay using mutant β-glucuronidase (GUS). Two types of antibodies (Mouse IgG1) against the target antigen are prepared, and the assay sample and the enzyme supplied with the kit are mixed and incubated for detection. For fluorescence measurement.
Storage -80°C CAS
Link

ONEPot Immunoassay Kit, Colorimetric <OpenGUS Method>

DatasheetThis may not be the latest data sheet.


  • Product Code: DS860
  • Supplier: BDL
  • Size: 1kit
  • Price: $480

Description Kit for constructing a protein assay system. Assay is based on the principle of OpenGUS Immunoassay using mutant β-glucuronidase (GUS). Two types of antibodies (Mouse IgG1) against the target antigen are prepared, and the assay sample and the enzyme supplied with the kit are mixed and incubated for detection. For absorbance measurement.
Storage -80°C CAS
Link

Go back to index

CONTACT

export@funakoshi.co.jp

  • ※Prices on our website are for your reference only. Please inquire your distributor for your prices.
  • ※Please note that Product Information or Price may change without notice.