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HOME> Products> Cell Culture> Cell Detachment, Dissociation Solution> Brightase-C ＜Recombinant Collagenase＞

For Primary Cell Isolation! High-Purity, Novel Collagenase Brightase-C ＜Recombinant Collagenase＞

Date：May 14 2026Web Page No：160779

Nippi, Inc.

Brightase-C exterior

This recombinant enzyme is produced by expressing the collagenase gene from Grimontia hollisae in Brevibacillus choshinensis — a Gram-positive bacterium that lacks an outer membrane containing lipopolysaccharides (LPS). Manufactured using animal-derived component-free (ADCF) materials, it maintains a strictly controlled endotoxin level of 10 EU/mg or less. It exhibits higher collagenolytic activity than Clostridium histolyticum-derived collagenase and is uniquely capable of cleaving Type VI collagen.

Nipp also offers Brightase-TH, a recombinant thermolysin expressed in Brevibacillus choshinensis. For optimal results, a set product featuring both Brightase-C and Brightase-TH is available. This allows you to adjust the ratio of the two enzymes to achieve the most effective cell isolation according to the specific tissue type being processed.

＊The products are for research use only.

Features

  • Capable of cleaving Type VI collagen, which cannot be digested by conventional Clostridium histolyticum-derived collagenase.
  • Ideal for isolating cells from biomaterials, serving as the starting point for regenerative medicine research.
  • Animal Origin-Free (AOF) — no animal-derived materials are used throughout the manufacturing process, including cultivation.
  • Low endotoxin levels (10 EU/mg or less).
  • No caseinolytic activity (free of casein-degrading activity).

＊Since Brightase-C does not contain neutral protease, we highly recommend using it in combination with Brightase-TH when isolating various cells from biological tissues, such as in islet isolation or adipose-derived stem cell extraction.


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Technical Data

Expression System

Fig. 1: Production of Recombinant Proteins Using the Brevibacillus Expression System
Production of Recombinant Proteins Using the Brevibacillus Expression System

The Brevibacillus expression system used to produce Brightase-C (this product) offers key advantages: it is well-suited for extracellular protein expression, enables target protein purification directly from the culture supernatant, and is low in endotoxins as well as free of animal-derived components and antibiotics (neomycin).


Enzyme Stability

Stability Comparison Between Full-Length Enzyme and Catalytic Domain of Grimontia hollisae-Derived Collagenase
Stability Comparison Between Full-Length Enzyme and Catalytic Domain of Grimontia hollisae-Derived Collagenase

The 74 kDa full-length enzyme containing the PPC (pre-peptidase C-terminal) domain*1 undergoes autolysis, which cleaves it into a 62 kDa form and reduces its activity. In contrast, the 62 kDa enzyme possessing only the catalytic domain remains stable without undergoing autolysis, even after being incubated at 37°C for one day. Consequently, the 62 kDa enzyme (Brightase-C, this product) is the ideal choice for a collagenase product due to its superior enzymatic activity stability.

*1 PPC domain: Confirmed to function as a CBD (collagen-binding domain).


Lot-to-Lot Consistency

Consistent Collagenase Activity Across Batches

The average collagenase activity across four lots of Brightase-C (this product) was 8,717 ± 428 U/mg, demonstrating high consistency and minimal lot-to-lot variation.


Activity Comparison

Specific Activity Comparable to Competitor Product

When using Azocoll (azo-dye-impregnated insoluble collagen fibers) as a substrate, the specific activity of Brightase-C (this product) was comparable to that of Competitor Product A*2. This outcome is consistent with the results obtained from mouse pancreas digestion tests (data not shown).

*2 Clostridium-derived enzyme
＊View the research report: Development of recombinant collagenase from Grimontia hollisae.


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Product Information

[Date : May 21 2026 00:14]

Detail Product Name Product Code Supplier Size Price
Brightase-C
DatasheetThis may not be the latest data sheet. 		892431 NIPNippi, Inc. 40 mg $199

Description Brightase-C (recombinant Collagenase), This recombinant collagenase is produced by cloning the collagenase gene from Grimontia hollisae and expressing it in Brevibacillus. It is capable of cleaving Type VI collagen, which cannot be digested by conventional Clostridium histolyticum-derived collagenase. This product is ideal for isolating cells from biomaterials, serving as the essential starting point for regenerative medicine research.
Storage -20°C CAS
Link

Brightase-C
DatasheetThis may not be the latest data sheet. 		892432 NIPNippi, Inc. 2x40 mg $398

Description Brightase-C (recombinant Collagenase), This recombinant collagenase is produced by cloning the collagenase gene from Grimontia hollisae and expressing it in Brevibacillus. It is capable of cleaving Type VI collagen, which cannot be digested by conventional Clostridium histolyticum-derived collagenase. This product is ideal for isolating cells from biomaterials, serving as the essential starting point for regenerative medicine research.
Storage -20°C CAS
Link

[Date : May 21 2026 00:14]

Brightase-C

DatasheetThis may not be the latest data sheet.


  • Product Code: 892431
  • Supplier: NIP
  • Size: 40mg
  • Price: $199

Description Brightase-C (recombinant Collagenase), This recombinant collagenase is produced by cloning the collagenase gene from Grimontia hollisae and expressing it in Brevibacillus. It is capable of cleaving Type VI collagen, which cannot be digested by conventional Clostridium histolyticum-derived collagenase. This product is ideal for isolating cells from biomaterials, serving as the essential starting point for regenerative medicine research.
Storage -20°C CAS
Link

Brightase-C

DatasheetThis may not be the latest data sheet.


  • Product Code: 892432
  • Supplier: NIP
  • Size: 2x40mg
  • Price: $398

Description Brightase-C (recombinant Collagenase), This recombinant collagenase is produced by cloning the collagenase gene from Grimontia hollisae and expressing it in Brevibacillus. It is capable of cleaving Type VI collagen, which cannot be digested by conventional Clostridium histolyticum-derived collagenase. This product is ideal for isolating cells from biomaterials, serving as the essential starting point for regenerative medicine research.
Storage -20°C CAS
Link



Go back to index

Brightase-TH

Brightase-TH exterior

This recombinant enzyme is produced by expressing thermolysin in Brevibacillus choshinensis. Manufactured using animal component-free (ACF) raw materials, it maintains a strictly controlled endotoxin specification of 10 EU/mg or less. It is designed for isolating cells from biological tissues, making it ideal for applications such as islet isolation and adipose-derived stem cell extraction.

Brightase-TH (Recombinant Thermolysin)

[Date : May 21 2026 00:14]

Detail Product Name Product Code Supplier Size Price
Brightase-TH, ≧4mg
DatasheetThis may not be the latest data sheet. 		892441 NIPNippi, Inc. 4 mg $199

Description Brightase-TH (recombinant Thermolysin), This recombinant protein is produced by expressing thermolysin in Brevibacillus choshinensis. It is manufactured using animal component-free (ACF) raw materials and maintains a strictly controlled endotoxin specification of 10 EU/mg or less. It is designed for isolating cells from biological tissues, making it ideal for applications such as islet isolation and adipose-derived stem cell extraction.
Storage -20°C CAS
Link

Brightase-TH, ≧4mg
DatasheetThis may not be the latest data sheet. 		892442 NIPNippi, Inc. 2x4 mg $398

Description Brightase-TH (recombinant Thermolysin), This recombinant protein is produced by expressing thermolysin in Brevibacillus choshinensis. It is manufactured using animal component-free (ACF) raw materials and maintains a strictly controlled endotoxin specification of 10 EU/mg or less. It is designed for isolating cells from biological tissues, making it ideal for applications such as islet isolation and adipose-derived stem cell extraction.
Storage -20°C CAS
Link

[Date : May 21 2026 00:14]

Brightase-TH, ≧4mg

DatasheetThis may not be the latest data sheet.


  • Product Code: 892441
  • Supplier: NIP
  • Size: 4mg
  • Price: $199

Description Brightase-TH (recombinant Thermolysin), This recombinant protein is produced by expressing thermolysin in Brevibacillus choshinensis. It is manufactured using animal component-free (ACF) raw materials and maintains a strictly controlled endotoxin specification of 10 EU/mg or less. It is designed for isolating cells from biological tissues, making it ideal for applications such as islet isolation and adipose-derived stem cell extraction.
Storage -20°C CAS
Link

Brightase-TH, ≧4mg

DatasheetThis may not be the latest data sheet.


  • Product Code: 892442
  • Supplier: NIP
  • Size: 2x4mg
  • Price: $398

Description Brightase-TH (recombinant Thermolysin), This recombinant protein is produced by expressing thermolysin in Brevibacillus choshinensis. It is manufactured using animal component-free (ACF) raw materials and maintains a strictly controlled endotoxin specification of 10 EU/mg or less. It is designed for isolating cells from biological tissues, making it ideal for applications such as islet isolation and adipose-derived stem cell extraction.
Storage -20°C CAS
Link


Set product (Brightase-C and Brightase-TH)

[Date : May 21 2026 00:14]

Detail Product Name Product Code Supplier Size Price
Brightase-C/TH (40mg/≧4mg)
DatasheetThis may not be the latest data sheet. 		892451 NIPNippi, Inc. 1 set $398

Description This product consists of one vial of Brightase-C (40 mg) and one vial of Brightase-TH (≧4 mg). The Brightase-C/TH set contains recombinant collagenase derived from Grimontia hollisae and thermolysin derived from Bacillus thermoproteolyticus rokko. Both enzymes are packaged separately, allowing you to adjust them to the optimal ratio depending on the specific tissue type being targeted for cell isolation.
Storage -20°C CAS
Link

[Date : May 21 2026 00:14]

Brightase-C/TH (40mg/≧4mg)

DatasheetThis may not be the latest data sheet.


  • Product Code: 892451
  • Supplier: NIP
  • Size: 1set
  • Price: $398

Description This product consists of one vial of Brightase-C (40 mg) and one vial of Brightase-TH (≧4 mg). The Brightase-C/TH set contains recombinant collagenase derived from Grimontia hollisae and thermolysin derived from Bacillus thermoproteolyticus rokko. Both enzymes are packaged separately, allowing you to adjust them to the optimal ratio depending on the specific tissue type being targeted for cell isolation.
Storage -20°C CAS
Link

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