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HOME> Products> Cell Culture> Medium> Xyltech™ MSC-02 Animal-Free / Growth MSC
HOME> Products> Cell Culture> Stem cells> Xyltech™ MSC-02 Animal-Free / Growth MSC

Animal-Free, Serum-Free Culture Media for Controlling the Proliferation Speed of Human Mesenchymal Stem Cells Xyltech™ MSC-02 Animal-Free / Growth MSC

Date：July 16 2026Web Page No：160772

Bourbon Biomedical Advanced Research Laboratories, Inc. (BBARL, Inc.)

This is an animal-free (contains no animal-derived components) and serum-free culture medium optimized for human mesenchymal stem cells (hMSCs). By combining two types of media—Xyltech™ MSC-02 Animal-Free for growth suppression and Xyltech™ Growth MSC for cell expansion—the proliferation speed of human mesenchymal stem cells can be precisely controlled.

Changes in Cell Number of hADMSCs Cultured with Xyltech Growth MSC [for Expansion] and Xyltech MSC-02 Animal-Free [for Growth Suppression] — **Growth curve of hADMSCs cultured with Growth MSC [for expansion] & MSC-02 Animal-Free [for growth suppression]**

While cells are proliferated using Xyltech™ Growth H-Fbro in standard maintenance culture, it was confirmed that cells can be cultured with Xyltech™ H-Fbro-01 to suppress proliferation while maintaining cellular characteristics.

＊The products are for research use only.
＊ Xyltech is a trademark of BBARL, Inc.

About hMSCs
Features
Application Examples
Product Information

About hMSCs

Human mesenchymal stem cells (hMSCs) are a type of somatic stem cells that possess the capability to differentiate into specific cell lineages. They exist within tissues such as adipose tissue and bone marrow in the human body. Because they can differentiate into adipocytes, osteoblasts, chondrocytes, and other cell types, they are highly expected to be utilized in regenerative medicine applications, including the reconstruction of bone and skin tissues.

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Features

Xyltech™ MSC-02 Animal-Free (For Growth Suppression)

Proliferation Control: Suppresses cell growth while maintaining stem cell characteristics.
Reversible Effect: Cells resume rapid proliferation immediately after switching to the growth medium.
Animal-free formula containing no animal-derived components.
No Coating Required: Compatible with standard, non-coated tissue culture vessels.
Glucose-Free: Formulated without glucose.

Note: Neither medium contains trypsin inhibitors. Utilizing a trypsin inhibitor during cell passaging is strongly recommended to fully neutralize trypsin activity.

Xyltech™ Growth MSC (For Expansion)

Xyltech MSC-Growth — Basal medium and supplement

Efficient Expansion: Optimized for robust growth and maintenance of hMSCs.
Animal-free formula containing no animal-derived components.
No Coating Required: No special vessel coating is needed.
Two-Component System: Consists of a basal medium (87-331) and a separate supplement (87-333).

Note: Neither medium contains trypsin inhibitors. Utilizing a trypsin inhibitor during cell passaging is strongly recommended to fully neutralize trypsin activity.

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Application Examples

Cell Morphology

hADMSCs with Xyltech MSC-02 Animal-Free for 3 days — **Xyltech™ MSC-02 Animal-Free [For Growth Suppression]**

hADMSCs with Xyltech Growth MSC for 3 days — **Xyltech™ Growth MSC [For Expansion]**

Phase-contrast microscopic images of normal human adipose-derived mesenchymal stem cells (hADMSCs) cultured for 3 days in each medium.

Differentiation Potential Evaluation

Differentiation into adipocytes from hADMSCs cultured for 3 days in Xyltech MSC-01 Xeno-Free. — **Adipocyte Differentiation**
(Oil Red O staining)

Xyltech MSC-01 Xeno-Freeで3日間培養したヒト脂肪由来間葉系幹細胞の骨芽細胞への分化 — **Osteoblast Differentiation**
(Alizarin Red S staining)

Xyltech MSC-01 Xeno-Freeで3日間培養したヒト脂肪由来間葉系幹細胞の軟骨細胞への分化 — **Chondrocyte Differentiation**
(Alcian Blue staining)

Differentiation induction into various cell types was successfully performed using hADMSCs cultured for 3 days with Xyltech™ MSC-02 Animal-Free [For Proliferation Control]. The results confirmed that Xyltech™ MSC-02 Animal-Free effectively suppresses cell proliferation while maintaining the inherent stem cell properties.

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Product Information

Xyltech™ MSC-02 Animal-Free [For Growth Suppression]

[Date : July 22 2026 00:11]

Detail Product Name Product Code Supplier Size Price

Xyltech MSC-02 Animal-Free (#10402)
DatasheetThis may not be the latest data sheet. 87-334 KSKCell Science & Technology Institute, Inc. 100 ml $718

Contact

Description	An animal-free, serum-free culture medium that allows culture of human mesenchymal stem cells while suppressing their proliferation. This product is a culture medium suitable for suppressive growth control of human mesenchymal stem cells (hMSCs). Xyltech MSC-02 Animal-Free can be used to control the growth rate of hMSCs in combination with Xyltech™ Growth MSC, which is a serum-free medium for hMSCs proliferation. This product is an Animal-Free culture medium. In addition, there is no need to pre-coat culture vessels with any type of attachment matrix.
Storage		CAS
Link

[Date : July 22 2026 00:11]

Xyltech MSC-02 Animal-Free (#10402)

DatasheetThis may not be the latest data sheet.

Product Code: 87-334
Supplier: KSK
Size: 100ml
Price: $718

Description	An animal-free, serum-free culture medium that allows culture of human mesenchymal stem cells while suppressing their proliferation. This product is a culture medium suitable for suppressive growth control of human mesenchymal stem cells (hMSCs). Xyltech MSC-02 Animal-Free can be used to control the growth rate of hMSCs in combination with Xyltech™ Growth MSC, which is a serum-free medium for hMSCs proliferation. This product is an Animal-Free culture medium. In addition, there is no need to pre-coat culture vessels with any type of attachment matrix.
Storage		CAS
Link

Contact

Xyltech™ Growth MSC [For Expansion] (Basal Medium and Supplement)

[Date : July 22 2026 00:11]

Detail Product Name Product Code Supplier Size Price

Xyltech Growth MSC Medium (#10411)
DatasheetThis may not be the latest data sheet. 87-331 KSKCell Science & Technology Institute, Inc. 500 ml $231

Contact

Description	A xenofree, serum-free basal culture medium for maintenance culture of human mesenchymal stem cells. This product is a culture medium suitable for the growth of human mesenchymal stem cells (hMSCs). When used in combination with Xyltech MSC-01 Xeno-Free or Xyltech MSC-02 Animal-Free, hMSCs proliferation can be controlled as needed. In addition, there is no need to pre-coat culture vessels.
Storage	4°C,Dark Storage	CAS
Link

Xyltech Growth MSC Supplement AF (#10413)
DatasheetThis may not be the latest data sheet. 87-333 KSKCell Science & Technology Institute, Inc. 10 ml $794

Contact

Description	A culture medium supplement added to the animal-free, serum-free basal medium (#87-331) for maintenance culture of human mesenchymal stem cells.
Storage		CAS
Link

[Date : July 22 2026 00:11]

Xyltech Growth MSC Medium (#10411)

DatasheetThis may not be the latest data sheet.

Product Code: 87-331
Supplier: KSK
Size: 500ml
Price: $231

Description	A xenofree, serum-free basal culture medium for maintenance culture of human mesenchymal stem cells. This product is a culture medium suitable for the growth of human mesenchymal stem cells (hMSCs). When used in combination with Xyltech MSC-01 Xeno-Free or Xyltech MSC-02 Animal-Free, hMSCs proliferation can be controlled as needed. In addition, there is no need to pre-coat culture vessels.
Storage	4°C,Dark Storage	CAS
Link

Contact

Xyltech Growth MSC Supplement AF (#10413)

DatasheetThis may not be the latest data sheet.

Product Code: 87-333
Supplier: KSK
Size: 10ml
Price: $794

Description	A culture medium supplement added to the animal-free, serum-free basal medium (#87-331) for maintenance culture of human mesenchymal stem cells.
Storage		CAS
Link

Contact

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