T7 Promoter Protein Expression Vector in E. coli (pET Vector) pET Expression Vector
Date：October 06 2025Web Page No：160754
These are pET expression vectors that uses the highly efficient T7 promoter to express the desired protein in E. coli. There are high copy number vectors, medium copy number vectors, and vectors whose expression can be controlled by LacI, each containing a drug resistance gene for ampicillin or kanamycin.
pET System
The T7 expression system is a powerful expression system using a pET expression vector containing the T7 promoter and a lysogen of bacteriophage λDE3, such as the BL21 (DE3) E. coli strain, into which the T7 RNA polymerase gene is integrated.
The expression of the T7 RNA polymerase gene under the control of the lac UV5 promoter in the BL21 (DE3) E. coli genome is induced by the addition of isopropyl-1 thio-β-D-galactopyranoside (IPTG). The expressed T7 RNA polymerase recognizes the T7 promoter of the pET vector and strongly expresses the target protein sequence introduced downstream of the T7 promoter.
Feartures
- All six vectors share a common multi-cloning site. This makes it easy to subclone from one series to another.
- Because of the small size of the vectors, high-efficiency transformation can be expected even with large inserts.
- Proteins fused with an N-terminal His tag can be expressed. Untagged proteins can also be expressed by inserting the target gene at the Nde I site in the cloning site.
- Volume: 15 μg
Features of Each Vector
| Vector | pETUA (#DV200) |
pETBA (#DV210) |
pETIA (#DV215) |
pETUK (#DV220) |
pETBK (#DV230) |
pETIK (#DV235) |
|---|---|---|---|---|---|---|
| Drug resistance gene | Ampicillin | Kanamycin | ||||
| Replicon | pUC | pMB1 | pUC | pMB1 | ||
| Plasmid copy number | High (500-700 copies) | Middle (10-20 copies) | High (500-700 copies) | Middle (0-20 copies) | ||
| Addition of 6×His tag (N-terminus) | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ |
| Suppression of expression in non-induced state | － | － | ✓ | － | － | ✓ |
|
Features · Applications |
Plasmids are easily purified. Suitable for expression of proteins that are not toxic to the host. | Can be used to express a wide variety of proteins. | Suitable for expression of proteins that are toxic to the host because uninduced expression can be suppressed. | Plasmids are easily purified. Suitable for expression of proteins that are not toxic to the host. | Can be used to express a wide variety of proteins. | Suitable for expression of proteins that are toxic to the host because uninduced expression can be suppressed. |
| Size | 2,741 bp | 3,269 bp | 4,012 bp | 2,695 bp | 3,223 bp | 3,966 bp |
* When the effect of the protein to be expressed on the host E. coli is not clear, or when the choice of vector is difficult, try pETBA. If you want to suppress the expression in the uninduced state, use pETIA. When the protein to be expressed has little effect on the host E. coli, use pETUA, which has a large plasmid copy number, has the advantage of making miniprep and sequencing easier.
* pETUK, pETBK, and pETIK, which contain the kanamycin resistance gene, have the same characteristics as pETUA, pETBA, and pETIA, respectively. These vectors are useful for subcloning from ampicillin-resistant cloning vectors.
Example of Use
 |
M : DynaMarker Protein Eco (#DM610) 1 : pETUK (65 kDa protein) 2 : pETBA (65 kDa protein) 3 : pETUA (45 kDa protein) 4 : pETBA (44 kDa protein) 5 : pETBK (44 kDa protein) 6 : pETIK (30 kDa protein) |
Product Information
[Date : October 07 2025 00:09]
| Detail | Product Name | Product Code | Supplier | Size | Price | ||||||||||||||||||||||||||||||
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pET Expression Vector pETUA DatasheetThis may not be the latest data sheet. |
DV200 | BDLBioDynamics Laboratory Inc | 15 µg | $260 | |||||||||||||||||||||||||||||||
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pET Expression Vector pETBA DatasheetThis may not be the latest data sheet. |
DV210 | BDLBioDynamics Laboratory Inc | 15 µg | $260 | |||||||||||||||||||||||||||||||
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pET Expression Vector pETIA DatasheetThis may not be the latest data sheet. |
DV215 | BDLBioDynamics Laboratory Inc | 15 µg | $260 | |||||||||||||||||||||||||||||||
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pET Expression Vector pETUK DatasheetThis may not be the latest data sheet. |
DV220 | BDLBioDynamics Laboratory Inc | 15 µg | $260 | |||||||||||||||||||||||||||||||
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pET Expression Vector pETBK DatasheetThis may not be the latest data sheet. |
DV230 | BDLBioDynamics Laboratory Inc | 15 µg | $260 | |||||||||||||||||||||||||||||||
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pET Expression Vector pETIK DatasheetThis may not be the latest data sheet. |
DV235 | BDLBioDynamics Laboratory Inc | 15 µg | $260 | |||||||||||||||||||||||||||||||
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[Date : October 07 2025 00:09]
pET Expression Vector pETUA
DatasheetThis may not be the latest data sheet.
- Product Code: DV200
- Supplier: BDL
- Size: 15µg
- Price: $260
| Description |
pETUA is a high copy number, ampicillin resistant, T7 bacterial expression vector. The T7 expression system is one of the strongest expression systems and has been widely used with a coupling of BL21 (DE3) E. coli strain. |
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| Storage | CAS | ||
| Link |
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pET Expression Vector pETBA
DatasheetThis may not be the latest data sheet.
- Product Code: DV210
- Supplier: BDL
- Size: 15µg
- Price: $260
| Description |
pETBA is a medium copy number, ampicillin resistant, T7 bacterial expression vector. The T7 expression system is one of the strongest expression systems and has been widely used with a coupling of BL21 (DE3) E. coli cell. |
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| Storage | CAS | ||
| Link |
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pET Expression Vector pETIA
DatasheetThis may not be the latest data sheet.
- Product Code: DV215
- Supplier: BDL
- Size: 15µg
- Price: $260
| Description | |||
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| Storage | CAS | ||
| Link |
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pET Expression Vector pETUK
DatasheetThis may not be the latest data sheet.
- Product Code: DV220
- Supplier: BDL
- Size: 15µg
- Price: $260
| Description |
pETUK is a high copy number, kanamycin resistant, T7 bacterial expression vector. The T7 expression system is one of the strongest expression systems and has been widely used with a coupling of BL21 (DE3) E. coli strain. |
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| Storage | CAS | ||
| Link |
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pET Expression Vector pETBK
DatasheetThis may not be the latest data sheet.
- Product Code: DV230
- Supplier: BDL
- Size: 15µg
- Price: $260
| Description |
pETBK is a medium copy number, kanamycin resistant, T7 bacterial expression vector. The T7 expression system is one of the strongest expression systems and has been widely used with a coupling of BL21 (DE3) E. coli strain. |
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| Storage | CAS | ||
| Link |
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pET Expression Vector pETIK
DatasheetThis may not be the latest data sheet.
- Product Code: DV235
- Supplier: BDL
- Size: 15µg
- Price: $260
| Description |
pETIK is a medium copy number, kanamycin resistant, stringent controllable T7 bacterial expression vector. The T7 expression system is one of the strongest expression systems and has been widely used with a coupling of BL21 (DE3) E. coli cell. |
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| Storage | CAS | ||
| Link |
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