Competent Cells that can be Dispensed & Refrozen ⋅ Operation 6 minutes ⋅ High Transformation Efficiency DynaCompetent Cells JetGiga DH5α
Date：October 08 2025Web Page No：160692
Transformation efficiency of Giga (109) level even after thawing and refreezing (dispensing)!
Features
- 2 transformation methods can be selected depending on your experiment (operation time is less than 10 minutes in both cases. See below).
- Ultra-high transformation efficiency: > 1×109 CFU/μg (pUC19) (with heat shock).
- Can be refrozen after dispensing to the desired volume.
- Refreezing can be done with a deep freezer! No need for liquid nitrogen or dry ice!
- Blue-white screening is possible (for plasmid vector containing lacZ gene).
Two Ways to Use it
| Heat shock | Time required | Recovery culture | Transformation efficiency | Features |
|---|---|---|---|---|
| Yes | 6 minutes | Not needed | ＞1×109 CFU/μg |
|
| No | 10 minutes | Need (5 minutes) |
＞1×108 CFU/μg |
|
Dispensing and Restorage Procedure
- Cool the dispensing tube and pipette tip in advance.
- Thaw the competent cell on ice water.
- Dispense into a desired volume.
- Refreeze in a deep freezer (-80°C).
* For details, please refer to the data sheet attached to the product.
Example of Use
Comparison with other rapid transformation-competent cells
The pUC19 plasmid was transformed using the transformation method recommended by each company. The efficiency of this competent cells (#DS230) were well above 1×109 CFU/μg, which was higher than that of other companies. The colonies on the plate were clearly large and aligned.
Changes in transformation efficiency of the competent cells during freeze-thaw cycles
The product was thawed on ice and refrozen in a deep freezer. The product sufficiently maintained 1×109 CFU/μg after 1 freeze/thaw.
Product Information
[Date : October 18 2025 00:08]
| Detail | Product Name | Product Code | Supplier | Size | Price | ||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
DynaCompetent Cells JetGiga DH5α DatasheetThis may not be the latest data sheet. |
DS230 | BDLBioDynamics Laboratory Inc | 10x100 µl | $210 | |||||||||||||||||||||||||||||||
|
|
|
||||||||||||||||||||||||||||||||||
[Date : October 18 2025 00:08]
DynaCompetent Cells JetGiga DH5α
DatasheetThis may not be the latest data sheet.
- Product Code: DS230
- Supplier: BDL
- Size: 10x100µl
- Price: $210
| Description |
DH5α competent cells with high transformation efficiency. Transformation efficiency: 1×10@9 CFU/μg in the 6-minute method (with heat shock), 1×10@8 CFU/μg in 10-minute method (without heat shock). |
||
|---|---|---|---|
| Storage | CAS | ||
| Link |
|
||
CONTACT
export@funakoshi.co.jp
- ※Prices on our website are for your reference only. Please inquire your distributor for your prices.
- ※Please note that Product Information or Price may change without notice.