SARS Spike Glycoprotein-specific Neutralizing Antibody Useful for Novel Coronavirus (SARS-CoV-2 / COVID-19) Research Anti-SARS Spike Glycoprotein Antibody (Clone 3A2)
Date：April 10 2015Web Page No：160198
Anti-SARS Coronavirus Spike monoclonal antibody is useful for novel coronaviruses (SARS-CoV-2 / COVID-19) study. It specifically reacts with and neutralizes SARS Spike Glycoprotein, but not SARS-CoV-2 or MERS-CoV.
- Validation Date with Immunofluorescent (IF)
- Specification
- Example of Use
- Citation
- Product Information
Validation Date with Immunofluorescent (IF)
This antibody reacts well with SARS virus (SARS-CoV) but not with novel coronavirus (SARS-CoV-2) or MERS virus (MERS-CoV).
SARS Virus
Specification
- Clone : 3A2
- Immunogen : inactivated SARS virus
- Cross- Reactiv:ity : SARS spike glycoprotein. Not react with SARS-CoV-2 and MERS-CoV
- Applications : Western blotting (10,000-fold dulution), Immunofluoresece staining (1,000-fold dulution), Flow Cytometry, Neutralization
- Purification : Protein A-purified
Example of Use
Identification of the spike antigen in SARS virus infected cells by indirect immunostaining with
3A2 antibody at 1,000 fold dilution
(a) Uninfected Vero E6 cells, (b) SARS virus infected Vero E6 cells
Identification of the spike glycoprotein in the crude extract of the SARS virus infected Vero cells by Western blotting
10,000 fold dilution. The molecular weight of the S protein is larger because of the sugar chain.
SARS virus spike protein is 1,181 amino acids and has a predicted molecular weight of 132 kDa, however SDS-PAGE shows approximately 200 kDa due to the inclusion of sugar chains.
Citation
- Yamate M, et al. Microbes Infect., 7(15):1530～1540(2005). PMID: 16269264. IF, FCM
"Establishment of Vero E6 cell clones persistently infected with severe acute respiratory syndrome coronavirus." - Yamashita M, et al. Biochem. Biophys. Res. Commun., 19;334(1):79～85(2005). PMID:15992768. IF
"Susceptibility of human and rat neural cell lines to infection by SARS-coronavirus." - Li GM, et al. Jpn. J. Infect Dis., 61(2):123～127(2008). PMID:18362400. WB, Neutralization
"Reduced incorporation of SARS-CoV spike protein into viral particles due to amino acid substitutions within the receptor binding domain."
* FCM : Flow cytometry, IF : Immunofluorescence, WB : Western blotting
Product Information
[Date : April 26 2025 00:07]
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Anti-SARS spike glycoprotein antibody (clone 3A2) DatasheetThis may not be the latest data sheet. |
65-101 | BAKBioAcademia, Inc. | 50 µg | $165 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||
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[Date : April 26 2025 00:07]
Anti-SARS spike glycoprotein antibody (clone 3A2)
DatasheetThis may not be the latest data sheet.
- Product Code: 65-101
- Supplier: BAK
- Size: 50µg
- Price: $165
| Description | |||
|---|---|---|---|
| Storage | -20°C | ||
| Antigen Species | Host Species | Mouse | |
| Class | Label | Unlabeled | |
| Cross Reactivity | Application | FCM,IC,IF,Western Blot,Neutralising | |
| Clonality | Format | ||
| Purification | Absorption | ||
| Link |
Antibody Product List (N-Z) |
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