DNAメチル化酵素関連抗体 DNA Methylation Related Antibody

Epigentek社では、エピジェネティクス関連因子に対する抗体を豊富に取りそろえています。

※ 本製品は研究用です。研究用以外には使用できません。

抗5-Hydroxymethylcytosine抗体(#A-1018)の使用例
Dot Blot analysis of A-1018 with DNA oligos containing cytosine (C), 5-methylcytosine (5-mC), or 5-hydroxymethylcytosine (5-hmC). 10 ng of C, 5-mC, and 5-hmC DNA oligos each were spotted on a membrane. The membrane was incubated with 0.5 μg/ml of Epigentek's 5-hmC Clone HMC/4D9 antibody (1:2000 dilution) then with peroxidase conjugated goat anti-mouse IgG (dilution 1/3000). Specific signal was only observed with 5-hmc DNA oligos.

抗5-Hydroxymethylcytosine抗体(#A-1018)の使用例
Demonstration of high sensitivity and specificity of Epigentek's 5-hmC Clone HMC/4D9 antibody against 5-hmC DNA. Synthetic DNA oligos containing 5-hmC, 5-mC or only cytosine, respectively were added into the assay wells at different concentrations and then specific signals was only detected with 5-hmC DNA.

抗5-Hydroxymethylcytosine抗体(#A-1018)の使用例
Selective enrichment of hydroxymethyated DNA. 50 pg of unmethylated, methylated, and hydroxymethylated DNA oligos were each spiked into fragmented human genomic DNA (500 ng). hMeDIP was processed with Epigentek's 5-hmC Clone HMC/4D9 antibody. Eluted DNA was analyzed by real time PCR with the primers to detect the presence of spiked DNA oligos. Fold-enrichment represents the amount of recovered DNA oligos and was calculated based on the Cts.

抗5-Hydroxymethylcytosine抗体(#A-1019)の使用例
Dot blot assay shows that 5-Hydroxymethylcytosine Antibody specifically recognized 5-hydroxymethyl Cytidine (hmC). Lane 1: 10 pmol, Lane 2: 2 pmol; Lane 3: 0.4 pmol.

抗5-Hydroxymethylcytosine抗体(#A-1019)の使用例
5-Hydroxymethylcytosine Selective enrichment of hydroxymethyated DNA with an anti-5-hydroxymethylcytidine antibody (Cat. No. A-1019). 50 pg of unmethylated, methylated, and hydroxymethylated DNA control were each spiked into fragmented human genomic DNA (500 ng). hMeDIP was processed with the 5-hmC antibody. Eluted DNA was analyzed by real time PCR to detect the presence of spiked control DNA. %input represents the amount of recovered control DNA and was calculated based on the Cts.

抗5-Methylcytosine抗体(#A-1014)の使用例
Methylated DNA successfully captured by anti-5-methylcytosine clone 33D3 (Cat #A-1014) during MeDIP.

抗5-Methylcytosine抗体(#A-1014)の使用例
Immunofluorescence staining with anti-5-methylcytosine clone 33D3 (Cat #A-1014).

抗TET1抗体(#A-1020)の使用例
Western blot analysis of TET1 expression in non-transfected:(1) and human TET1 transfected:(2) 293T whole cell lysates.

抗TRIM28抗体(#A-1108)の使用例
Western Blot detection against Immunogen (42.06 KDa).