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Worthington社 プロテアーゼ製品ラインナップ

掲載日情報:2018/05/02 現在Webページ番号:63772

Worthington社の豊富なプロテアーゼのラインナップをご紹介します。目的のアプリケーションで製品を選択して下さい。

Partially Purified for Tissue Dissociation and Protein Digestion

「†」マークは酵素の切断部位を示しています。

Enzyme
(Molecular Weight KDa)
Specificity pH Optimum Extinction Coefficient E1%, 280nm< Common Substrates Activators Inhibitors Product/Applications
この列のリンクをクリックすると別タブで価格表が開きます。
Collagenase
(68~130)
-Pro-X-†-Gly-Pro-Y-
X = neutral
Y = nonspecific
6.3~7.5 13.20 (ColHG, Theoretical)
13.40 (ColHG, Theoretical)
Collagen FALGPA Wünsch Ca2+, Zn2+ alpha-2-macroglobulin Cysteine, histidine
DTT, 2-mercapto
EDTA, EGTA
Hg2+ & other heavy metal ions
ortho-phenanthroline
Collagenase products
Tissue dissociation/ Primary cell isolation applications
Elastase
(25.9)
Elastin, -X-†-Y-
X = uncharged, nonaromatic
Y = nonspecific
8.0~8.5 21.8 (Theoretical) Casein Denatured collagen Elastin, Fibrin Suc-Ala3-NA None required alpha-antitrypsin
DFP
alpha-2-macroglobulin
PMSF
ES/ESL, suspension/lyo powder
Tissue dissociation/ Primary cell isolation applications.
Neutral Protease
(36)
-X-†-Leu/Phe-†-Y-
X/Y = nonspecific
5.9~7.0 13.96 (Theoretical) BAEE Casein Ca2+, Mg2+, Mn2+, Fe2+, and Al3+ EDTA, EGTA
Hg2+ & other heavy metal ions
ortho-phenanthroline
NPRO/NPRO2
Tissue dissociation/ Primary cell isolation and cell harvesting applications.
Papain
(23)
-X-†-Y-
X = nonspecific but Arg, Lys and Phe preferred
Y = nonspecific
6.0~7.0 22.88 (Theoretical) BAEE Cysteine
EDTA
Reducing agents
GSH, NBS
AEBSF, Antipain
Cystatin, Leupeptin
alpha-2-macroglobulin
Hg2+ & other heavy metal ions
DFP, PMSF
PAP/PAPL, suspension/lyo powder
Neural tissue dissociation/primary cell isolation applications
(see Tissue Dissociation Guide for specific references).
Antibody cleavage
RBC modification
Pepsin
(34.6)
-X-†-Y-
X = nonspecific but aromatic & hydrophobic preferred
Y ≠ Ala, Gly, Val
1.0~4.0
unstable ≥5
14.39
(Theoretical)
Casein
Hemoglobin
None required Pepstatin A
Diazoketones
Epoxides
PM
Collagen bioprocessing/purification
Antibody cleavage
Proteinase K
(28.9)
-X-†-Y-
X = nonspecific but aliphatic, aromatic & hydrophobic preferred
Y = nonspecific
7.5~12 8.23 (trypsinogen, theoretical)
37,000 (trypsin and trypsinogen, theoretical)
8.69 (trypsin, theoretical)
Casein
Hemoglobin
Keratin
Ca2+
Active in 0.5~1% SDS
DFP
EGTA
PMSF
PROK, PROKS
DNA/RNA purification
Trypsin
(23.8)
-X-†-Y-
X = Arg, Lys
Y = nonspecific
7.5~8.5 14.3 BAEE
Casein
TAME
Ca2+
Lanthanide
Aprotinin, Benzamidine
DFP, EDTA, Leupeptin
alpha-2-macroglobulin
PMSF, TLCK
Trypsin Inhibitors (LBI, OI, SI/SIC)
Trypsin products
Protein Digestion/Sequencing (purified)
Tissue dissociation/Primary cell isolation applications
(see Tissue Dissociation Guide for specific references)

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Proteases for Protein Sequencing

Enzyme
(Molecular Weight KDa)
Specificity pH Optimum Extinction Coefficient E1%, 280nm< Common Substrates Activators Inhibitors Product/Applications
この列のリンクをクリックすると別タブで価格表が開きます。
Carboxy-peptidase B
(34.3)
H2-N-Rn-Y-†-X-COOH
X = basic amino acids (Arg, Lys, Orn)
Y = nonspecific
7.0~9.0 21.4 (Folk 1971) Hippuryl-L-arginine None required EDTA
Hg2+ & other heavy metal ions
EDTA, EGTA ortho-phenanthroline
COBC/COBPMS
Sequence analysis by successive cleavage of C-terminal basic amino acids Insulin production
Carboxy-peptidase Y
(64)
H2-N-Rn-Y-†-X-COOH
X,Y = nonspecific
4.5~6.0 15
(Hayashi et al. 1973, and Kuhn et al. 1973)
ATEE
Bz-Phe-Ala-Leu
Z-Phe-Ala
None required APCK, Aprotinin
DFP
4-Hydroxy mercuribenzoate
PMSF
COY
C-terminal sequencing & Modification/labeling of peptides and proteins
Chymotrypsin TLCK treated
(25.6)
-X-†-Y-
X = aromatic
Y = nonspecific
7.8~8.0 20.57
(Theoretical)
ATEE
BTEE
Ca2+ alpha-antitrypsin
Aprotinin
DFP, PMSF, TPCK
alpha-2-macroglobulin
CDSEQ/CDTLCK
Sequence analysis
Peptide synthesis, mapping/fingerprinting
Endo-Arg-C
(Clostripain)
(53)
-Arg-†-Y-
Y = nonspecific
7.4~7.8 16.57
(Theoretical)
BAEE Ca2+
Reducing agents
EDTA, TLCK, Tris
Hg2+ & other heavy metal ions
CPSEQ, CP
Peptide mapping & synthesis
Sequence analysis
Hydrolysis / condensation of amide bonds
Endo-Glu-C (Staph. Protease V8)
(27)
-Glu-†-Y-
(NH4 buffers pH 4, 7.8)
-Asp-†-Y-
(PO4 buffer pH 7.8)
4.0 & 7.8 4.26
(Houmard 1976)
Casein
Z-Phe-Leu-Glu-4NA
None required DFP
F-, Cl-, Br-, CH3COO-
NO3-
alpha-2-macroglobulin
STSEQ, STAP
Peptide mapping & sequence analysis
SequENZ™ Trypsin, Sequencing Grade, Modified
(23.8)
-X-†-Y-
X = Arg, Lys
Y = nonspecific
7.5~8.5 14.3 BAEE
Casein
TAME
Ca2+
Lanthanide
Aprotinin, Benzamidine
DFP, EDTA, Leupeptin
alpha-2-macroglobulin-
PMSF, TLCK
Trypsin Inhibitors (egg white, lima bean, pancreatic, soybean)
TRSEQZ, Modified Sequencing Grade
chemically modified to reduce autolysis
Peptide mapping & sequence analysis
Cleavage fusion proteins
Trypsin, Sequencing Grade, Native
(23.8)
TRSEQII, Sequencing Grade, Native
Peptide mapping & sequence analysis
Cleavage fusion proteins
Trypsin, TPCK Treated
(23.8)
TRTPCK, TPCK Treated
Peptide mapping & sequence analysis
Cleavage fusion proteins

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