Worthington社 プロテアーゼ製品ラインナップ
掲載日情報:2018/05/02 現在Webページ番号:63772
Worthington社の豊富なプロテアーゼのラインナップをご紹介します。目的のアプリケーションで製品を選択して下さい。
※組織・細胞分散の参考になる使用文献を検索できるTissue Dissociation Guideはこちら
※酵素・生理活性物質マニュアルWorthington Enzyme Manual はこちら
※本製品は研究用です。研究用以外には使用できません。
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Partially Purified for Tissue Dissociation and Protein Digestion
※「†」マークは酵素の切断部位を示しています。
Enzyme (Molecular Weight KDa) |
Specificity | pH Optimum | Extinction Coefficient E1%, 280nm< | Common Substrates | Activators | Inhibitors | Product/Applications ※この列のリンクをクリックすると別タブで価格表が開きます。 |
---|---|---|---|---|---|---|---|
Collagenase (68~130) |
-Pro-X-†-Gly-Pro-Y-
X = neutral Y = nonspecific |
6.3~7.5 | 13.20 (ColHG, Theoretical) 13.40 (ColHG, Theoretical) |
Collagen FALGPA Wünsch | Ca2+, Zn2+ | alpha-2-macroglobulin Cysteine, histidine DTT, 2-mercapto EDTA, EGTA Hg2+ & other heavy metal ions ortho-phenanthroline |
Collagenase products Tissue dissociation/ Primary cell isolation applications |
Elastase (25.9) |
Elastin, -X-†-Y-
X = uncharged, nonaromatic Y = nonspecific |
8.0~8.5 | 21.8 (Theoretical) | Casein Denatured collagen Elastin, Fibrin Suc-Ala3-NA | None required | alpha-antitrypsin DFP alpha-2-macroglobulin PMSF |
ES/ESL, suspension/lyo powder Tissue dissociation/ Primary cell isolation applications. |
Neutral Protease (36) |
-X-†-Leu/Phe-†-Y-
X/Y = nonspecific |
5.9~7.0 | 13.96 (Theoretical) | BAEE Casein | Ca2+, Mg2+, Mn2+, Fe2+, and Al3+ | EDTA, EGTA Hg2+ & other heavy metal ions ortho-phenanthroline |
NPRO/NPRO2 Tissue dissociation/ Primary cell isolation and cell harvesting applications. |
Papain (23) |
-X-†-Y-
X = nonspecific but Arg, Lys and Phe preferred Y = nonspecific |
6.0~7.0 | 22.88 (Theoretical) | BAEE | Cysteine EDTA Reducing agents GSH, NBS |
AEBSF, Antipain Cystatin, Leupeptin alpha-2-macroglobulin Hg2+ & other heavy metal ions DFP, PMSF |
PAP/PAPL, suspension/lyo powder Neural tissue dissociation/primary cell isolation applications (see Tissue Dissociation Guide for specific references). Antibody cleavage RBC modification |
Pepsin (34.6) |
-X-†-Y-
X = nonspecific but aromatic & hydrophobic preferred Y ≠ Ala, Gly, Val |
1.0~4.0 unstable ≥5 |
14.39 (Theoretical) |
Casein Hemoglobin |
None required | Pepstatin A Diazoketones Epoxides |
PM Collagen bioprocessing/purification Antibody cleavage |
Proteinase K (28.9) |
-X-†-Y-
X = nonspecific but aliphatic, aromatic & hydrophobic preferred Y = nonspecific |
7.5~12 | 8.23 (trypsinogen, theoretical) 37,000 (trypsin and trypsinogen, theoretical) 8.69 (trypsin, theoretical) |
Casein Hemoglobin Keratin |
Ca2+ Active in 0.5~1% SDS |
DFP EGTA PMSF |
PROK, PROKS DNA/RNA purification |
Trypsin (23.8) |
-X-†-Y-
X = Arg, Lys Y = nonspecific |
7.5~8.5 | 14.3 | BAEE Casein TAME |
Ca2+ Lanthanide |
Aprotinin, Benzamidine DFP, EDTA, Leupeptin alpha-2-macroglobulin PMSF, TLCK Trypsin Inhibitors (LBI, OI, SI/SIC) |
Trypsin products Protein Digestion/Sequencing (purified) Tissue dissociation/Primary cell isolation applications (see Tissue Dissociation Guide for specific references) |
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Proteases for Protein Sequencing
Enzyme (Molecular Weight KDa) |
Specificity | pH Optimum | Extinction Coefficient E1%, 280nm< | Common Substrates | Activators | Inhibitors | Product/Applications ※この列のリンクをクリックすると別タブで価格表が開きます。 |
---|---|---|---|---|---|---|---|
Carboxy-peptidase B (34.3) |
H2-N-Rn-Y-†-X-COOH
X = basic amino acids (Arg, Lys, Orn) Y = nonspecific |
7.0~9.0 | 21.4 (Folk 1971) | Hippuryl-L-arginine | None required | EDTA Hg2+ & other heavy metal ions EDTA, EGTA ortho-phenanthroline |
COBC/COBPMS Sequence analysis by successive cleavage of C-terminal basic amino acids Insulin production |
Carboxy-peptidase Y (64) |
H2-N-Rn-Y-†-X-COOH
X,Y = nonspecific |
4.5~6.0 | 15 (Hayashi et al. 1973, and Kuhn et al. 1973) |
ATEE Bz-Phe-Ala-Leu Z-Phe-Ala |
None required | APCK, Aprotinin DFP 4-Hydroxy mercuribenzoate PMSF |
COY C-terminal sequencing & Modification/labeling of peptides and proteins |
Chymotrypsin TLCK treated (25.6) |
-X-†-Y-
X = aromatic Y = nonspecific |
7.8~8.0 | 20.57 (Theoretical) |
ATEE BTEE |
Ca2+ | alpha-antitrypsin Aprotinin DFP, PMSF, TPCK alpha-2-macroglobulin |
CDSEQ/CDTLCK Sequence analysis Peptide synthesis, mapping/fingerprinting |
Endo-Arg-C (Clostripain) (53) |
-Arg-†-Y-
Y = nonspecific |
7.4~7.8 | 16.57 (Theoretical) |
BAEE | Ca2+ Reducing agents |
EDTA, TLCK, Tris Hg2+ & other heavy metal ions |
CPSEQ, CP Peptide mapping & synthesis Sequence analysis Hydrolysis / condensation of amide bonds |
Endo-Glu-C (Staph. Protease V8) (27) |
-Glu-†-Y-
(NH4 buffers pH 4, 7.8) -Asp-†-Y- (PO4 buffer pH 7.8) |
4.0 & 7.8 | 4.26 (Houmard 1976) |
Casein Z-Phe-Leu-Glu-4NA |
None required | DFP F-, Cl-, Br-, CH3COO- NO3- alpha-2-macroglobulin |
STSEQ, STAP Peptide mapping & sequence analysis |
SequENZ™ Trypsin, Sequencing Grade, Modified (23.8) |
-X-†-Y-
X = Arg, Lys Y = nonspecific |
7.5~8.5 | 14.3 | BAEE Casein TAME |
Ca2+ Lanthanide |
Aprotinin, Benzamidine DFP, EDTA, Leupeptin alpha-2-macroglobulin- PMSF, TLCK Trypsin Inhibitors (egg white, lima bean, pancreatic, soybean) |
TRSEQZ, Modified Sequencing Grade chemically modified to reduce autolysis Peptide mapping & sequence analysis Cleavage fusion proteins |
Trypsin, Sequencing Grade, Native (23.8) |
TRSEQII, Sequencing Grade, Native Peptide mapping & sequence analysis Cleavage fusion proteins |
||||||
Trypsin, TPCK Treated (23.8) |
TRTPCK, TPCK Treated Peptide mapping & sequence analysis Cleavage fusion proteins |
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