抗DNMT3A (64B1446)抗体 | Anti- DNMT3A (64B1446) antibody
掲載日情報：2018/08/30 現在Webページ番号：57784
世界最大級の抗体製品数を取り扱うNovus Biologicals社のDNMT3A (64B1446)に対する抗体(anti-DNMT3A (64B1446) | antibody DNMT3A (64B1446))です。Novus Biologicals社の抗体は数多くの学術論文で使用実績があります。
※本製品は研究用です。研究用以外には使用できません。
PE, AlexaFluor, FITC, DyLight などの蛍光を含む、さまざまな標識体も取り扱っています。
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Image
-Western-Blot-NB120-13888-img0020.jpg) | Western Blot: DNMT3A Antibody (64B1446) [NB120-13888] - Analysis of (A) Dnmt3a transfected 293 cell lysate and (B) untransfected 293 cell lysate using Dnmt3a antibody at 1 ug/ml. |
-Western-Blot-NB120-13888-img0015.jpg) | Biological Strategies Validation. Western Blot: DNMT3A Antibody (64B1446) [NB120-13888] - NTERA-2 cells were treated with Zebularine as indicated for 24 hours. Cell lysates were prepared and separated on a 7.5% gel by SDS-PAGE. Protein was transferred to PVDF membrane and blocked in 5% non-fat milk. The membrane was probed with 2 ug/ml anti-Dnmt3a in 1% milk, and detected with an anti-mouse HRP secondary antibody using chemiluminescence. Note the decrease in Dnmt3a expression upon treatment with 500 uM Zebularine (arrowhead). Additional bands at 90 and 75 kDa can also be detected with this antibody and may represent alternative splice variants. Tubulin is shown as a loading control |
-Immunohistochemistry-Paraffin-NB120-13888-img0012.jpg) | Immunohistochemistry-Paraffin: DNMT3A Antibody (64B1446) [NB120-13888] - IHC-P detection of DNMT3A on human hepatocellular carcinoma tissue section (NBP2-30221) using 1:100 dilution of DNMT3A antibody (clone 64B1446). The antibody generated a specific cytoplasmic staining in all the cancer cells while some of the cells depicted nuclear staining also. |
-Immunocytochemistry-Immunofluorescence-NB120-13888-img0016.jpg) | Immunocytochemistry/Immunofluorescence: DNMT3A Antibody (64B1446) [NB120-13888] - Ntera2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-DNMT3A (68B1446) [NB120-13888] at a 1:200 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. |
-Flow-Cytometry-NB120-13888-img0021.jpg) | Flow Cytometry: DNMT3A Antibody (64B1446) [NB120-13888] - Using the PE direct conjugate An intracellular stain was performed on NTERA-2 cells with DNMT3A (64B1446) NB120-13888PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin. |
-Western-Blot-NB120-13888-img0022.jpg) | Western Blot: DNMT3A Antibody (64B1446) [NB120-13888] - 1ug of recombinant purified DNMT3A protein. This image was submitted via customer Review. |
-Immunocytochemistry-Immunofluorescence-NB120-13888-img0017.jpg) | Immunocytochemistry/Immunofluorescence: DNMT3A Antibody (64B1446) [NB120-13888] - Expression of Dnmt3a in the nuclei of post-mitotic neurons in the olfactory epithelium (OE). DNMT3a (red) is co-expressed with neuron-specific tubulin (green) throughout the development of the olfactory epithelium. (Data courtsey of A. Jane Roskams, University of British Columbia). |
-Immunohistochemistry-Paraffin-NB120-13888-img0019.jpg) | Immunohistochemistry-Paraffin: DNMT3A Antibody (64B1446) [NB120-13888] - Formalin-fixed, paraffin-embedded human liver stained with Dnmt3a antibody at 5 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min. |
-Flow-Cytometry-NB120-13888-img0026.jpg) | Flow Cytometry: DNMT3A Antibody (64B1446) [NB120-13888] - An intracellular stain was performed on Jurkat cells with DNMT3A (64B1446) NB120-13888AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647. |
-Flow-Cytometry-NB120-13888-img0018.jpg) | Flow Cytometry: DNMT3A Antibody (64B1446) [NB120-13888] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using Dnmt3a antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used. |
-Flow-Cytometry-NB120-13888-img0023.jpg) | Flow Cytometry: DNMT3A Antibody (64B1446) [NB120-13888] - An intracellular stain was performed on HepG2 cells with DNMT3A (64B1446) NB120-13888APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to allophycocyanin. |
-Flow-Cytometry-NB120-13888-img0024.jpg) | Flow Cytometry: DNMT3A Antibody (64B1446) [NB120-13888] - An intracellular stain was performed on HepG2 cells with DNMT3A (64B1446) NB120-13888AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647. |
-Flow-Cytometry-NB120-13888-img0025.jpg) | Flow Cytometry: DNMT3A Antibody (64B1446) [NB120-13888] - An intracellular stain was performed on HeLa cells with DNMT3A (64B1446) NB120-13888APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to allophycocyanin. |
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Background
Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Recently, it has been shown that naturally occurring mutations of Dnmt3b gene occurs in patients with a rare autosomal recessive disorder, termed ICF (immunodeficiency, centromeric instability, and facial anomalies) syndrome.カートに商品を
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価格
[在庫・価格 ：2024年05月18日 00時00分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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Anti-Dnmt3a, Mouse-Mono(64B1446) |
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101 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Anti-Dnmt3a, Mouse-Mono(64B1446) |
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56 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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[在庫・価格 ：2024年05月18日 00時00分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
Anti-Dnmt3a, Mouse-Mono(64B1446)
文献数： 101
- 商品コード：NB120-13888
- メーカー：NOV
- 包装：0.1mg
- 価格：¥103,000
- 在庫：無(未発注)
- 納期：3～4週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
レビューあり。旧IMGENEX社 商品コード：IMG-268A，Keywords：64B1446 clone|DNA (cytosine-5-)-methyltransferase 3 alpha|DNA (cytosine-5)-methyltransferase 3A|DNA cytosine methyltransferase 3A2|DNA methyltransferase HsaIIIA|DNA MTase HsaIIIA|Dnmt3a|DNMT3A2|EC 2.1.1.37|M.HsaIIIA クローン：64B1446 Genbank No： 1788 Protein Accession No： Q9Y6K1 |
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| 別包装品 | 別包装品あり | ||||||
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| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | 免疫動物 | Mouse | |||||
| 交差性 | Human/Mouse/Rat | 適用 | ChIP,FCM,IC,IF,IHC,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 |
DNA メチレーションおよびクロマチン修飾研究用抗体 IHCPro 抗DNMT3A (64B1446)抗体 | Anti-DNMT3A (64B1446) Antibody |
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Anti-Dnmt3a, Mouse-Mono(64B1446)
文献数： 56
- 商品コード：NB120-13888SS
- メーカー：NOV
- 包装：0.025mg
- 価格：¥48,000
- 在庫：無(未発注)
- 納期：3～4週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
Keywords：DNA (cytosine-5-)-methyltransferase 3 alpha|DNA (cytosine-5)-methyltransferase 3A|DNA cytosine methyltransferase 3A2|DNA methyltransferase HsaIIIA|DNA MTase HsaIIIA|Dnmt3a|DNMT3A2|EC 2.1.1.37|M.HsaIIIA クローン：64B1446 Genbank No： 1788 Protein Accession No： Q9Y6K1 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | 免疫動物 | Mouse | |||||
| 交差性 | Human/Mouse | 適用 | ChIP,FCM,IC,IF,IHC,Western Blot | ||||
| 標識 | Unlabeled | 性状 | |||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
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カートに商品を
追加しました。
追加しました。
標識済み抗体価格
[在庫・価格 ：2024年05月18日 00時00分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
| 詳細 | 商品名 |
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Anti-DNMT3A, Mouse-Mono(64B1446), FITC |
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0 | |||
[在庫・価格 ：2024年05月18日 00時00分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
Anti-DNMT3A, Mouse-Mono(64B1446), FITC
文献数： 0
- 商品コード：NB120-13888F
- メーカー：NOV
- 包装：0.1ml
- 価格：¥127,000
- 在庫：無(未発注)
- 納期：3～4週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
クローン：64B1446 Genbank No： 1788 |
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| 法規制等 | |||
| 保存条件 | 法規備考 | ||
| 抗原種 | 免疫動物 | Mouse | |
| 交差性 | Human/Mouse | 適用 | FCM,IC,IF,IHC |
| 標識 | FITC | 性状 | Protein A/G Affinity Purified |
| 吸収処理 | クラス | IgG | |
| クロナリティ | Monoclonal | フォーマット | |
| 掲載カタログ |
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| 製品記事 | |||
| 関連記事 | |||
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製品情報は掲載時点のものですが、価格表内の価格については随時最新のものに更新されます。お問い合わせいただくタイミングにより製品情報・価格などは変更されている場合があります。
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