13,14-dihydro-15-keto Prostaglandin E2 MaxSpec Standard | CAS:363-23-5

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• 製品情報
• 製品詳細
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価格

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製品情報

13,14-dihydro-15-keto Prostaglandin E₂ (13,14-dihydro-15-keto PGE₂) is the primary metabolite of PGE₂ (Item Nos. 14010 | 10007211) in plasma. Endogenous or infused PGE₂ is rapidly metabolized by the enzymes 15-hydroxy PGDH and 15-oxo-prostaglandin Δ¹³-reductase to form 13,14-dihydro-15-keto PGE₂. 13,14-dihydro-15-keto PGE₂ accumulates to detectable levels; plasma levels in humans are between 10-100 pg/ml. It undergoes further metabolism and chemical decomposition, giving it a relatively short half-life. In dogs, the plasma half-life of 13,14-dihydro-15-keto PGE₂ is about 9 minutes. In humans the metabolite has a similar short half-life, making it a poor choice of analytes for assays designed to measure total PGE₂ biosynthesis.

13,14-dihydro-15-keto PGE₂ MaxSpec^® standard is a quantitative grade standard of 13,14-dihydro-15-keto PGE₂ (Item No. 14650) that has been prepared specifically for mass spectrometry or any application where quantitative reproducibility is required. The solution has been prepared gravimetrically and is supplied in a deactivated glass ampule sealed under argon. The concentration was verified by comparison to an independently prepared calibration standard. This 13,14-dihydro-15-keto PGE₂ MaxSpec^® standard is guaranteed to meet identity, purity, stability, and concentration specifications and is provided with a batchspecificcertificate of analysis. Ongoing stability testing is performed to ensure the concentration remains accurate throughout the shelf life of the product. Note: The amount of solution added to the vial is in excess ofthe listed amount. Therefore, it is necessary to accurately measure volumes for preparation of calibration standards. Follow recommended storage and handling conditions to maintain product quality.

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製品詳細

name	13,14-dihydro-15-keto Prostaglandin E2 MaxSpec Standard
Formal Name	9,15-dioxo-11α-hydroxy-prost-5Z-en-1-oic acid
CAS Number	363-23-5
Synonyms	13,14-dihydro-15-keto PGE2
Molecular Formula	C20H32O5
Formula Weight	352.5
Purity	>95%
Formulation	A solution in methyl acetate
SMILES	CCCCCC(=O)CC[C@H]1C(O)CC(=O)C1C/C=C\CCCC(=O)O
Storage	-20°C
Shipping	Wet ice in continental US; may vary elsewhere
Stability	≥ 1 year

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文献

1. Hamberg, M., and Samuelsson, B. On the metabolism of prostaglandins E1 and E2 in man. J. Biol. Chem. 246(22), 6713-6721 (1971).

2. Bothwell, W., Verburg, M., Wynalda, M., et al. A radioimmunoassay for the unstable pulmonary metabolites of prostaglandin E1 and E2: An indirect index of their in vivo disposition and pharmacokinetics. J. Pharmacol. Exp. Ther. 220(2), 229-235 (1982).

3. Leonhardt, A., Krauss, M., Gieler, U., et al. In vivo formation of prostaglandin E1 and prostaglandin E2 in atopic dermatitis. Br. J. Dermatol. 136(3), 337-340 (1997).

4. Fitzpatrick, F.A., Aguirre, R., Pike, J.E., et al. The stability of 13,14-dihydro-15 keto-PGE2. Prostaglandins 19(6), 917-931 (1980).

5. Granström, E., Hamberg, M., Hansson, G., et al. Chemical instability of 15-keto-13,14-dihydro-PGE2: The reason for low assay reliability. Prostaglandins 19(6), 933-945 (1980).

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