Prostaglandin E2 Ethanolamide MaxSpec Standard | CAS:194935-38-1

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価格

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製品情報

Prostaglandin E₂ ethanolamide (PGE₂-EA) is an analog of PGE₂ (Item No. 14010) with improved water solubility and stability. PGE₂-EA is formed via COX-2 metabolism of arachidonoyl ethanolamide (AEA; Item No. 90050) and acts as an agonist at E prostanoid (EP) receptors 1-4 (K_is = 2.45, 0.46, 0.2, and 0.51 μM, respectively). It also inhibits indoleamine 2,3-dioxygenase-1 (IDO-1) in THP-1 cells and human monocytes (IC₅₀s = 5.7 and 4.7 μM, respectively). PGE₂-EA (10 μM) prevents morphological changes and F-actin rearrangement as well as reduces L-homocysteine-induced NLRP3 inflammasome formation and activation in podocytes. Ex vivo, PGE₂-EA reduces luminal damage and lymphocyte infiltration in a human mucosal explant colitis model.

PGE₂-EA MaxSpec^® standard is a quantitative grade standard of PGE₂-EA (Item No. 14012) that has been prepared specifically for mass spectrometry or any application where quantitative reproducibility is required. The solution has been prepared gravimetrically and is supplied in a deactivated glass ampule sealed under argon. The concentration was verified by comparison to an independently prepared calibration standard. This PGE₂-EA MaxSpec^® standard is guaranteed to meet identity, purity, stability, andconcentration specifications and is provided with a batch-specific certificate of analysis. Ongoing stability testing is performed to ensure the concentration remains accurate throughout the shelf life of the product.Note: The amount of solution added to the vial is in excess of the listed amount. Therefore, it is necessary to accurately measure volumes for preparation of calibration standards. Follow recommended storage and handling conditions to maintain product quality.

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製品詳細

name	Prostaglandin E2 Ethanolamide MaxSpec Standard
Formal Name	N-(2-hydroxyethyl)-9-oxo-11α,15S-dihydroxy-prosta-5Z,13E-dien-1-amide
CAS Number	194935-38-1
Synonyms	Dinoprostone Ethanolamide PGE2-EA Prostamide E2
Molecular Formula	C22H37NO5
Formula Weight	395.5
Purity	≥95%
Formulation	A solution in ethanol
SMILES	CCCCCC(O)/C=C/[C@H]1C(O)CC(=O)C1C/C=C\CCCC(=O)NCCO
Storage	-20°C
Shipping	Wet ice in continental US; may vary elsewhere
Stability	≥ 1 year

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文献

1. Kozak, K.R., Crews, B.C., Morrow, J.D., et al. Metabolism of the endocannabinoids, 2-arachidonylgycerol and anandamide, into prostaglandin, thromboxane, and prostacyclin glycerol esters and ethanolamides. J. Biol. Chem. 277(47), 44877-44885 (2002).

2. Liu, T., Li, R., Pan, T., et al. Intercellular transfer of the cellular prion protein. The Journal of Biological Chemisty 27(49), 47671-47678 (2002).

3. Costabile, M., Bassal, N.K., Gerber, J.P., et al. Inhibition of indoleamine 2,3-dioxygenase activity by fatty acids and prostaglandins: A structure function analysis. Prostaglandins Leukot. Essent. Fatty Acids 122, 7-15 (2017).

4. Li, G., Xia, M., Abais, J.M., et al. Protective action of anandamide and Its COX-2 metabolite against l-homocysteine-induced NLRP3 inflammasome activation and injury in podocytes. J. Pharmacol. Exp. Ther. 358(1), 61-70 (2016).

5. Nicotra, L.L., vu, M.D., Harvey, B.S., et al. Prostaglandin ethanolamides attenuate damage in a human explant colitis model. Prostaglandins Other Lipid Mediat. 100-101, 22-29 (2013).

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