抗Goat Anti-Rabbit IgG, pre-adsorbed (HRP)抗体 | Anti-Goat Anti-Rabbit IgG, pre-adsorbed (HRP) antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

GeneTex secondary antibodies detect rabbit primary antibodies in a variety of platforms. Shown here is a serial 1:1 dilution of control rabbit IgG protein 250ng starting total load Co-incubated with GeneTex HRP conjugated Goat anti Rabbit IgG antibody (GTX27090) and Dylight 649 conjugated goat anti Rabbit IgG antiody at 1:20K in blocking buffer.

GTX27090 secondary antibody was used at 1:40,000 in blocking buffer to detect a rabbit primary antibody by Western Blot. Anti p27 antibody (GTX48799, 1:1000 RT 30 minutes) showed detection of 0.1 μg of recombinant p27 protein. Lane 1: Molecular weight markers. Lane 2: MBP-p27 fusion protein (arrow; expected MW: 73.3 kDa). Lane 3: MBP alone. Protein was run on a 4-20% gel, then transferred to 0.45 μm nitrocellulose and blocked with 1% BSA-TTBS overnight at 4ºC. Blot was imaged on the VersaDoc MP 4000 imaging system (Bio-Rad).

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製品情報

Host	Goat
Clonality	Polyclonal
Isotype	IgG
Application	WB, Dot, ELISA, IHC
Reactivity	Rabbit

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:2,000 - 1:10,000
Dot	Assay dependent
ELISA	1:20,000 - 1:100,000
IHC	1:1,000 - 1:5,000

Not tested in other applications.

Specificity/Sensitivity	This antibody has been pre-adsorbed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep serum proteins to ensure minimal cross-reactivity. Cross-reactivity with other species, however, is possible.

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PROPERTIES

Form	Liquid
Buffer	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 , 10 mg/mL Bovine Serum Albumin (BSA) , 0.01% (w/v) Gentamicin Sulfate.
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Rabbit
Immunogen	Rabbit IgG whole molecule
Target Isotype	IgG
Purification	IgG fraction
Conjugation	Horseradish peroxidase(HRP)
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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