抗Rabbit Anti-Mouse IgG (HRP)抗体 | Anti-Rabbit Anti-Mouse IgG (HRP) antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET
• REFERENCEE(抜粋)

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

An ELISA plate is coated with 50 μL of a 6x-His recombinant protein conjugated BSA at concentration ranged from 0.003 μg/mL to 3 μg/mL. The coated protein is detected with anti-His antibody (GTX628921) at 0.5 ng/mL. Bound antibodies were further detected by using HRP-conjugated goat anti-mouse IgG (GTX213112-01).

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製品情報

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ELISA
Reactivity	Mouse

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:5000-1:10000
ELISA	Assay dependent

Not tested in other applications.

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PROPERTIES

Form	Liquid
Buffer	0.05M Tris, 0.15M NaCl (pH7.4), 1%BSA. 0.025% ProClin 300 was added as a preservative.
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	0.59 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Mouse
Immunogen	Highly purified whole mouse IgG
Target Isotype	IgG
Purification	Affinity purified with antigen Affinity Purified IgG
Conjugation	Horseradish peroxidase(HRP)
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms

Mouse IgG secondary antibody, antiMouse IgG antibody, anti-Mouse IgG antibody, Rabbit antiMouse IgG HRP, Rabbit anti-Mouse IgG HRP, anti Mouse IgG antibody, Rabbit anti Mouse IgG HRP

Background

Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/ml in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.

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REFERENCEE(抜粋)

Application Reference	Application/Reactivity
Pan CH et al. Aging (Albany NY) 2019; 11 (19) : 8085-8102 Oxidative stress-induced cellular senescence desensitizes cell growth and migration of vascular smooth muscle cells through down-regulation of platelet-derived growth factor receptor-beta.	Application : WB
Morquecho-Campos P et al. Appetite 2019; 143 : 104399 Impact of food odors signaling specific taste qualities and macronutrient content on saliva secretion and composition.	Application : ELISA
Yang SH et al. Artif Cells Nanomed Biotechnol 2019; 47 (1) : 3021-3028 CD24 expression indicates healthier phenotype and less tendency of cellular senescence in human nucleus pulposus cells.	Application : WB
Wang SH et al. PLoS One 2017; 12 (8) : e0183692 Expression and potential roles of sodium-potassium ATPase and E-cadherin in human gastric adenocarcinoma.
Lenka G et al. Sci Rep 2017; 7 : 42573 Identification of Methylation-Driven, Differentially Expressed STXBP6 as a Novel Biomarker in Lung Adenocarcinoma.	Application : WB

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