抗Goat Anti-Mouse IgG (HRP)抗体 | Anti-Goat Anti-Mouse IgG (HRP) antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET
• REFERENCEE(抜粋)

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	Various whole cell extracts were separated by 12% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
	Various amounts of denatured mouse IgG protein were separated by 12% SDS-PAGE, and the membrane was blotted with HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) diluted at 1:5000.
	E2F1 antibody [16G7] detects E2F1 protein at nucleus in mouse cervix by immunohistochemical analysis.Sample: Paraffin-embedded mouse cervix.E2F1 antibody [16G7] (GTX70163) diluted at 1:500.The signal was developed by Mouse IgG antibody (HRP) (GTX213111-01). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

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製品情報

Host	Goat
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, ELISA, IHC
Reactivity	Mouse

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	Assay dependent
ICC/IF	Assay dependent
IHC-P	1:100-1:1000
ELISA	Assay dependent
IHC	Assay dependent

Not tested in other applications.

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PROPERTIES

Form	Liquid
Buffer	0.05M Tris, 0.15M NaCl (pH7.4), 1%BSA. 0.025% ProClin 300 was added as a preservative.
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	0.28 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Mouse
Immunogen	Highly purified whole mouse IgG.
Target Isotype	IgG
Purification	Purified by antigen-affinity chromatography.
Conjugation	Horseradish peroxidase(HRP)
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms

Goat antiMouse IgG HRP, Mouse IgG secondary antibody, antiMouse IgG antibody, anti-Mouse IgG antibody, Goat anti-Mouse IgG HRP, anti Mouse IgG antibody, Goat anti Mouse IgG HRP

Background

Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/ml in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.

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REFERENCEE(抜粋)

Application Reference	Application/Reactivity
Kim S et al. Nat Commun 2020; 11 (1) : 612 PRMT6-mediated H3R2me2a guides Aurora B to chromosome arms for proper chromosome segregation.	Application : WB
Sun Y et al. Int Immunopharmacol 2020; 80 : 106198 Combined evaluation of the expression status of CD155 and TIGIT plays an important role in the prognosis of LUAD (lung adenocarcinoma).	Application : WB
Kim SH et al. Nutrients 2020; 12 (1) Fermented Morinda citrifolia (Noni) Alleviates DNCB-Induced Atopic Dermatitis in NC/Nga Mice through Modulating Immune Balance and Skin Barrier Function.	Application : WB
Koo B et al. J Pharm Pharmacol 2020; 72 (1) : 149-160 A botanical drug composed of three herbal materials attenuates the sensorimotor gating deficit and cognitive impairment induced by MK-801 in mice.	Application : WB
Kim HJ et al. Eur J Med Chem 2020; 185 : 111777 A novel chalcone derivative as Nrf2 activator attenuates learning and memory impairment in a scopolamine-induced mouse model.	Application : WB

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