抗Goat Anti-Rabbit IgG (DyLight594)抗体 | Anti-Goat Anti-Rabbit IgG (DyLight594) antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET
• REFERENCEE(抜粋)

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

目次に戻る

価格

目次に戻る

DATA IMAGES

	alpha Tubulin antibody detects alpha Tubulin protein at cytoskeleton by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Red: alpha Tubulin stained by alpha Tubulin antibody (GTX112141) diluted at 1:500.The signal was developed using Goat Anti-Rabbit IgG antibody (DyLight594) (GTX213110-05) diluted at 1:2000.Blue: Fluoroshield with DAPI (GTX30920).Scale bar= 10 μm.
	Immunofluorescence photomicrographs of frozen sections of mouse liver.Red: Histone H3K27me3 (trimethyl Lys27) antibody (GTX121184) diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight594) (GTX213110-05).Blue: Nuclear staining with Hoechst 33342.
	BrdU antibody (GTX128091) detects BrdU protein from HeLa treated with 10 μM BrdU for 30 minutes and fixed in 4% paraformaldehyde at 4ºC for 15 minutes by flow cytometry analysis. Black: Unlabelled sample was used as a control. Red: BrdU antibody (GTX128091) dilution: 1:50.The Rabbit IgG antibody (DyLight594) (GTX213110-05) was used to detect the primary antibody.

目次に戻る

製品情報

Host	Goat
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IHC-Fr, FACS
Reactivity	Human, Rabbit

目次に戻る

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	Assay dependent
ICC/IF	1:100-1:2000
IHC-P	Assay dependent
IHC-Fr	1:100-1:1000
FACS	1:50-1:200

Not tested in other applications.

目次に戻る

PROPERTIES

Form	Liquid
Buffer	100mM phosphate buffer (pH9.0)
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. Protect from light.
Concentration	Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species	Rabbit
Target Isotype	IgG
Purification	Purified by antigen-affinity chromatography.
Conjugation	DyLight594
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

目次に戻る

TARGET

Synonyms	Goat Anti Rabbit IgG

目次に戻る

REFERENCEE(抜粋)

Application Reference	Application/Reactivity
Ou J et al. Cell Mol Biol Lett 2019; 24 : 39 Non-contact co-culture with human vascular endothelial cells promotes epithelial-to-mesenchymal transition of cervical cancer SiHa cells by activating the NOTCH1/LOX/SNAIL pathway.
Yao-Shen Huang et al. bioRxiv 2019; (Epub) Sonogenetic modulation of cellular activities using an engineered auditory-sensing protein.
Wu JY et al. Cancers (Basel) 2019; 11 (5) YC-1 Antagonizes Wnt/帣-Catenin Signaling Through the EBP1 p42 Isoform in Hepatocellular Carcinoma.	Application : ICC/IF
Jing Q et al. J Cell Mol Med 2019; 23 (7) : 4711-4722 Wnt3a promotes radioresistance via autophagy in squamous cell carcinoma of the head and neck.	Application : ICC/IF
Huang PT et al. PLoS One 2015; 10 (2) : e0116372 Huntingtin-associated protein 1 interacts with breakpoint cluster region protein to regulate neuronal differentiation.	Application : ICC/IF

目次に戻る