抗IKK alpha (14A231) - Azide Free抗体 | Anti-IKK alpha (14A231) - Azide Free Antibody

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Image

	Western Blot: IKK alpha Antibody (14A231) - Azide Free [NBP2-27409] - analysis of IKK in Daudi cell lysate using IKKa monoclonal antibody at 1 ug/ml.
	Immunocytochemistry/Immunofluorescence: IKK alpha Antibody (14A231) - Azide Free [NBP2-27409] - IKK alpha antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). An antibody dilution of 1:10 was used. Image objective 40x.
	Immunohistochemistry-Paraffin: IKK alpha Antibody (14A231) - Azide Free [NBP2-27409] - Formalin-fixed, paraffin-embedded human kidney stained with IKKa antibody at 5 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
	Flow Cytometry: IKK alpha Antibody (14A231) - Azide Free [NBP2-27409] - Intracellular staining of HEK293 cells using 0.5 ug of IKKa antibody (red) and isotype control (green). Intracellular flow kit was used for this test, and an anti-mouse IgG1 PE conjugated secondary antibody.
	Simple Western: IKK alpha Antibody (14A231) - Azide Free [NBP2-27409] - Analysis using Azide/BSA FREE version of NBP2-27409. Simple Western lane view shows a specific band for IKK alpha in 0.5 mg/ml of Daudi (left) and U937 (right) lysate. This exeriment was performed under reducing conditions using the 12-230 kDa separation system.

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Background

NF-kB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitory proteins that mask the nuclear localization signal of NF-kB thereby preventing translocation of NF-kB to the nucleus. External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Synthesis of IkB is autoregulated. IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, Ikka, Ikk2/, and Ikk3/. In vitro, Ikka and Ikk2/ can form homo- and heterodimers that can phosphorylate IkBs at the regulatory serine residues directly. Ikka and Ikk2/ are phosphorylated by NF-kB-inducing kinase (NIk) and MAP kinase kinase kinase-1 (MEkk1) (1), respectively. Targeted disruption of Ikka gene in mice results in skin and limb abnormalities and death of newborns (2,3).

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