抗Calreticulin抗体 | Anti-Calreticulin Antibody

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	Western Blot: Calreticulin Antibody [NB600-103] - Detection of calreticulin in human kidney lysate using NB 600-103 at 1:1,000. ECL exposure, 10 seconds.
	Immunocytochemistry/Immunofluorescence: Calreticulin Antibody [NB600-103] - IF Confocal analysis of 3T3 cells using Calreticulin antibody (NB600-103, 1:20). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).
	Immunohistochemistry-Paraffin: Calreticulin Antibody [NB600-103] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human prostate cancer using Calreticulin antibody at 1:20 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic staining in glands was observed. Staining was performed by Histowiz.
	Western Blot: Calreticulin Antibody [NB600-103] - Western blot analysis of HeLa and C6 cell lysate using calreticulin antibody at 1:1000.
	Simple Western: Calreticulin Antibody [NB600-103] - Simple Western lane view shows a specific band for Calreticulin in 0.2 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

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Background

Calreticulin (CRT) is a ubiquitous multifunctional calcium-binding protein which attaches to the surface of cells and implicates in signal transduction events associated with innate immunity, cell adhesion, and apoptosis. Because CRT lacks a trans-membrane domain, CD91 (LDLR-related protein 1) has been proposed as a candidate anchoring receptor for this protein. CRT is mainly localized in ER lumen, cytoplasmic granules as well as in sarcoplasmic reticulum lumen as a monomer where it forms a component of an EIF2 complex (CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5) and interacts with GABARAP, NR3C1, PDIA3/ERp57, TRIM21 etc. CRT is a calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in ER via the calreticulin/calnexin cycle. It interacts with DNA-binding domain of NR3C1 for facilitating its nuclear export. CRT is involved in maternal gene expression regulation and participates in oocyte maturation via regulation of calcium homeostasis. In immune system, cell surface CRT (csCRT) serves as the signal-transducing receptor for collectin family proteins which binds to foreign organisms or apoptotic cells through their globular heads, while their collagen-like tails bind to csCRT, which leads to phagocytosis. CRT located in the cytoplasm, at the cell membrane and extracellular matrix functions in many physiological processes, such as cell adhesion, phagocytosis, and tumor recognition.

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