抗Carbonic Anhydrase IX/CA9抗体 | Anti-Carbonic Anhydrase IX/CA9 Antibody

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	Immunohistochemistry-Paraffin: Carbonic Anhydrase IX/CA9 Antibody [NB100-417] - IHC analysis of a formain fixed paraffin embedded (FFPE) tissue section of human breast cancer using CAIX antibody at 1:1000 dilution. The primary antibody bound to CAIX antigens in the tissue section was detected using a HRP labeled secondary antibody and DAB reagent. Nuclei of the cells were counterstained with hematoxylin. This CAIX antibody generated an expected cytoplasmic staining of CAIX protein with an intense signal around the cellular membranes in tumor cores. The latter are more likely to be hypoxic in growing tumors which signifies that the observed CAIX staining is specific.
	Western Blot: Carbonic Anhydrase IX/CA9 Antibody [NB100-417] - Analysis in 1) HeLa, 2) MDA-MB-231 and 3) A549 whole cell lysates.
	Biological Strategies Validation. Immunocytochemistry/Immunofluorescence: Carbonic Anhydrase IX/CA9 Antibody [NB100-417] - Immunofluorescence of human RCC tumor cryosections using NB100-417 (Panel A). Panel B shows staining with normal rabbit serum.
	Immunohistochemistry-Frozen: Carbonic Anhydrase IX/CA9 Antibody [NB100-417] - Analysis of human colon carcinoma, xenografted in mice. Image courtesy of an anonymous product review.
	Genetic Strategies Validation. Immunocytochemistry/Immunofluorescence: Carbonic Anhydrase IX/CA9 Antibody [NB100-417] - Analysis of HRP conjugate of NB100-417. HEK 293 cells using NB100-417. Panel A shows CAIX-transfected cells (epitope-unmasked with SDS) and panel B shows mock transfected cells.
	Immunohistochemistry: Carbonic Anhydrase IX/CA9 Antibody [NB100-417] - Analysis using the Biotin conjugate. Staining of Renal carcinoma tissue.
	Simple Western: Carbonic Anhydrase IX/CA9 Antibody [NB100-417] - Simple Western lane view shows a specific band for CAIX in 0.1 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
	Genetic Strategies Validation. Western Blot: Carbonic Anhydrase IX/CA9 Antibody [NB100-417] - Analysis using the HRP conjugate. Analysis in transfected HEK cell lysate. Rabbit IgG was used as a negative control.
	Immunocytochemistry/Immunofluorescence: Carbonic Anhydrase IX/CA9 Antibody [NB100-417] - Analysis using the DyLight 488 conjugate of NB100-417. Staining of Carbonic Anhydrase IX (red) in human glioma U87 cells. DAPI counterstains nuclei (blue). Image provided via product review by verified customer.
	Western Blot: Carbonic Anhydrase IX/CA9 Antibody [NB100-417] - Anaysis in transfected HEK cell lysate using NB 100-417. Rabbit IgG was used as a negative control.
	Western Blot: Carbonic Anhydrase IX/CA9 Antibody [NB100-417] - Analysis on rat renal cortex.
	Western Blot: Carbonic Anhydrase IX/CA9 Antibody [NB100-417] - Analysis on various human cell lysates. Image from verified customer review.

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Background

Carbonic anhydrase IX (CAIX) is a member of the Carbonic anhydrase family which are enzymes that assists rapid interconversion of carbon dioxide and water into carbonic acid, protons, and bicarbonate ions. They are abundant in all mammalian tissues and because of their functionality, they have become an important diagnostic marker for various cancers, most notably renal cell carcinoma (RCC). Carbonic Anhydrase IX (CA IX) is one of the most hypoxically inducible genes because of its stability and the location of the expressed protein within the membrane. Carbonic anhydrases have a widespread role in regulating pH in normal tissues, by regulating hydrogen ion (H+) flux. The pH is important in cell death under hypoxia, thus a blockade of CA IX (CA9) results in increased cell death under hypoxia. Therefore, CA IX has become a reliable histochemical marker of hypoxia.

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