抗EGLN1/PHD2抗体 | Anti-EGLN1/PHD2 Antibody

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	Western Blot: EGLN1/PHD2 Antibody [NB100-137] - Detection of Human PHD2 by Western Blot. Samples : Recombinant epitope-tagged PHD1, PHD2 or PHD3 (10 ng/lane) or whole cell lysate from HeLa cells. Antibody : Affinity purified rabbit anti-PHD2 used at 1 ug/ml. Detection : Chemiluminescence.
	Immunocytochemistry/Immunofluorescence: EGLN1/PHD2 Antibody [NB100-137] - Staining of endogenous PHD2 in U2OS cells. Image from verified customer review.
	Immunohistochemistry: EGLN1/PHD2 Antibody [NB100-137] - Staining of Vascular Endothelium 40X in Lung.
	Flow Cytometry: EGLN1/PHD2 Antibody [NB100-137] - Detection of PHD2. 1 million Jurkat cells were fixed, permeabilized, and stained with 3.0 ug/ml anti-PHD2 NB100-137 in a 150 ul reaction. Isotype control (black), anti-MLL1 (red).
	Western Blot: EGLN1/PHD2 Antibody [NB100-137] - Analysis of PHD2 expression in human prostate cancer cell lines. Image from verified customer review.
	Western Blot: EGLN1/PHD2 Antibody [NB100-137] - Analysis of PHD2 in Hep3B cell lysate.
	Western Blot: EGLN1/PHD2 Antibody [NB100-137] - Detection of PHD2 human glioblastoma cells. Image from verified customer review.
	Western Blot: EGLN1/PHD2 Antibody [NB100-137] - Analysis of PHD2 in normoxia (lane 1) or hypoxia (lane 2) treated SK-N-BE(2) cells using anti-PHD2 antibody. Image from verified customer review.
	Immunocytochemistry/Immunofluorescence: EGLN1/PHD2 Antibody [NB100-137] - Staining of PHD2 in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
	Simple Western: EGLN1/PHD2 Antibody [NB100-137] - Simple Western lane view shows a specific band for PHD2/HIF Prolyl Hydroxylase 2 in 0.5 mg/ml of Hypoxic HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

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Background

PHD2/HIF Prolyl Hydroxylase 2 is one of 4 PHD (PHD1-4) enzymes that function as oxygen sensors and are responsible for the post-translational modification of HIF-1alpha, a component of a transcriptional complex involved in oxygen homeostasis. During normoxic levels, PHDs catalyze the hydroxylation of prolyl residues on HIF-1alpha and target it for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. The PHD isoforms appear to function in a non-redundant manner and may differ in their expression patterns and their catalytic selectivity. Alternate names for PHD2/HIF Prolyl Hydroxylase 2 include PHD2, PH2, Prolyl hydroxylase domain containing protein 2, HIF2PH2, HIF-Prolyl hydroxylase 2, EGLN1, egl nine homolog 1, and C1orf12.

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