Independent Antibodies Validation and Genetic Strategies Validation.Western Blot: HIF-1 alpha Antibody [NB100-134] - Analysis of HIF-1 alpha in overexpression and endogenous HIF1 alpha & HIF2 alpha using anti-HIF-1 alpha antibody. The data showed that HIF-1 alpha antibody did not react to HIF-2 alpha overexpression. Image from verified customer review.
Biological Strategies Validation. Western Blot: HIF-1 alpha Antibody [NB100-134] - Analysis of NB100-134, anti-HIF-1 alpha, on normoxic and hypoxic nuclear rat cell lysates.
Independent Antibodies Validation and Biological Strategies Validation.Immunoprecipitation: HIF-1 alpha Antibody [NB100-134] - PC-3 Cell Lysates run with Cobalt Chloride treatments. Immunoprecipitation was performed with protein A/G Agarose beads. This data was provided courtesy of Kelie Reece, Figg lab, NCI.
Biological Strategies Validation. Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody [NB100-134] - Analysis of HIF-1 alpha in ARPE-19 cells using anti-HIF-1 alpha antibody. Image from verified customer review.
Immunohistochemistry-Paraffin: HIF-1 alpha Antibody [NB100-134] - Staining in the canine CL on days 10 to 70 after ovulation. PC = positive control (human placenta). NC = negative control. Image from confirmed customer review.
Simple Western: HIF-1 alpha Antibody [NB100-134] - Simple Western lane view shows a specific band for HIF-1 alpha in 0.2 mg/ml of Hypoxic HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Immunohistochemistry-Paraffin: HIF-1 alpha Antibody [NB100-134] - IHC analysis of a formalin-fixed paraffin-embedded tissue section of human endometrium carcinoma AN3CA cell line based xenograft using rabbit polyclonal HIF1 alpha antibody at 1:300 dilution. The signal was developed using HRP-labelled secondary antibody and DAB reagent, and the section was further counterstained using hematoxylin. The tested section depicted mainly a diffused cytoplasmic staining but there were some cells which showed nuclear signal also (representing hypoxic cells).
Immunohistochemistry-Paraffin: HIF-1 alpha Antibody [NB100-134] - Analysis of HIF-1 alpha on Zebrafish retina tissue using HIF-1 alpha antibody. Mouse tissue was used as a control. Image from verified customer review.
Western Blot: HIF-1 alpha Antibody [NB100-134] - 20 ug whole cell lysates (human ovarian cancer cell lines). Image from verified customer review.
Biological Strategies Validation. Western Blot: HIF-1 alpha Antibody [NB100-134] - Analysis of HIF-1 alpha in human Retinal and Choroidal primary endothelia cells using anti-HIF-1 alpha antibody. Image from verified customer review.
Immunohistochemistry: HIF-1 alpha Antibody [NB100-134] - Staining of human kidney, renal tubular epithelium in Cortex.
HIF-1 alpha (HIF1A) is a nuclear protein involved in mammalian oxygen homeostasis. This occurs as a posttranslational modification by prolyl hydroxylation. HIF-1 is a heterodimer composed of HIF-1 alpha and HIF-1 beta subunits. Both subunits are constantly translated. However, under normoxic conditions, human HIF-1 alpha is hydroxylated at Pro402 or Pro564 by a set of HIF prolyl hydroxylases, is polyubiquinated, and eventually degraded in proteosomes. Under hypoxic conditions, the lack of hydroxylation prevents HIF degradation and increases transcriptional activity. Therefore, the concentration of HIF-1 alpha increases in the cell.