抗HIF-2 alpha/EPAS1 (ep190b)抗体 | Anti-HIF-2 alpha/EPAS1 (ep190b) Antibody

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	Biological Strategies Validation. Simple Western: HIF-2 alpha/EPAS1 Antibody (ep190b) [NB100-132] - Simple Western lane view shows a specific band for HIF-2 alpha in 0.5 mg/ml of Hypoxic HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
	Biological Strategies Validation. Western Blot: HIF-2 alpha/EPAS1 Antibody (ep190b) [NB100-132] - HRP conjugated HIF-2 alpha detected in hypoxic human lysate. Lane 1: normoxic A549 lysate control, lane 2: hypoxic A549 lysate.
	Immunofluorescence: HIF-2 alpha/EPAS1 Antibody (ep190b) [NB100-132] - Analysis of HIF-2 alpha in human endometrium using anti-HIF-2 alpha antibody. Donkey anti-mouse Alexa Fluor 488 secondary antibody was used. Image from verified customer review.
	Flow Cytometry: HIF-2 alpha/EPAS1 Antibody (ep190b) [NB100-132] - HIF-2 alpha antibody was tested at 1:400 in HepG2 cells using an Alexa Fluor 488 secondary (shown in purple). M1 is defined by unstained cells.
	Western Blot: HIF-2 alpha/EPAS1 Antibody (ep190b) [NB100-132] - Analysis of HIF-2 alpha stabilization over time in 791T cells following exposure to hypoxia. [PMID: 23785417] Image using the HRP form of this antibody.
	Immunohistochemistry-Paraffin: HIF-2 alpha/EPAS1 Antibody (ep190b) [NB100-132] - Analysis of HIF-2 in human cardiac myocytes.
	Biological Strategies Validation. Western Blot: HIF-2 alpha/EPAS1 Antibody (ep190b) [NB100-132] - Western blot analysis of HepG2 without Cobalt (II) Chloride (1), HepG2 with Cobalt (II) Chloride (2), HepG2 normoxic (3), HepG2 hypoxic (4), HepG2 without Cobalt (II) Chloride (5), HepG2 with Cobalt (II) Chloride (6), HepG2 normoxic (7), and HepG2 hypoxic (8) using HIF-2 alpha antibody (NB100-132) at 1 - 2 ug/ml.
	Western Blot: HIF-2 alpha/EPAS1 Antibody (ep190b) [NB100-132] - C57B6 5 week old Mouse Kidney Tissue 60ug. Image from verified customer review.
	Immunohistochemistry-Paraffin: HIF-2 alpha/EPAS1 Antibody (ep190b) [NB100-132] - Analysis of HIF-2 alpha in paraffin-embedded mouse kidney tissue using anti-HIF-2 alpha antibody. Image from verified customer review.

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Background

Hypoxia-inducible factors (HIFs) generates heterodimers composed of alpha-subunit (HIF-1 alpha, HIF-2 alpha and HIF-3 alpha) and beta subunit (Arnt1, Arnt2 and Arnt3). Of these isoforms, HIF-1 alpha and HIF-2 alpha play a critical role in the cellular response to hypoxic conditions. After heterodimerization with beta subunits and binding to DNA sequence 5'-[AG]CGTG-3' within HRE (hypoxia response element) of target gene promoters, HIF-2 alpha regulates the gene expression in response to oxygen homeostasis alterations. HIF-2 alpha regulates VEGF expression for involving in the development of new blood vessels as well as the tubular system of lung. HIF-2 alpha play a role in the formation of the endothelium that gives rise to blood brain barrier. HIF-2 alpha is a strong activator of expression of Tie-2 tyrosine kinase which is a major player in angiogenesis. HIF-2alpha activation require recruitment of transcriptional coactivators such as CREBPB, EP300 etc. and its interaction with redox regulatory protein APEX mediates activation of CTAD. HIF-2 alpha/HIF-1alpha expression increases in certain malignancies, wherein, HIF-1 alpha plays both pro- as well as anti-proliferative activities, whereas, HIF-2 alpha is suggested to exert anti-proliferative activity only.

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