抗HIF-2 alpha/EPAS1抗体 | Anti-HIF-2 alpha/EPAS1 Antibody

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	Genetic Strategies Validation. Western Blot: HIF-2 alpha/EPAS1 Antibody [NB100-122] - Western blot of 786-O cells without or with VHL overexpression. Image from verified customer review.
	Independent Antibodies Validation, Biological Strategies Validation and Genetic Strategies Validation.Western Blot: HIF-2 alpha/EPAS1 Antibody [NB100-122] - Analysis of HIF-2 alpha in MDA-MB-231 cell lysate (overexpression and endogenous samples) using anti-HIF-2 alpha antibody. The data showed that HIF-2 alpha antibody did not react to HIF-1 alpha overexpression. Image from verified customer review.
	Immunocytochemistry/Immunofluorescence: HIF-2 alpha/EPAS1 Antibody [NB100-122] - HIF-2 alpha antibody was tested in RCC4 cells at a 1:200 dilution against Dylight 488 (Green). Alpha tubulin and nuclei were counterstained against Dylight 568 (Red) and DAPI (Blue), respectively.
	Biological Strategies Validation. Western Blot: HIF-2 alpha/EPAS1 Antibody [NB100-122] - Analysis of HIF-2 alpha in human Retinal and Choroidal primary endothelia lysates using anti-HIF-2 alpha antibody. Image from verified customer review.
	Biological Strategies Validation. Western Blot: HIF-2 alpha/EPAS1 Antibody [NB100-122] - Analysis using the HRP conjugate of NB100-122. Detection of normoxic and hypoxic nuclear rat cell lysates.
	Biological Strategies Validation. Western Blot: HIF-2 alpha/EPAS1 Antibody [NB100-122] - Analysis of HIF-2 alpha on Lane 1, Cobalt chloride treated COS7 nuclear extracts and Lane 2, Untreated COS7 nuclear extracts using NB100-122.
	Western Blot: HIF-2 alpha/EPAS1 Antibody [NB100-122] - HIF2 expression in HIF2 over-expressed HEK 293T cells. Image from verified customer review.
	Immunohistochemistry-Paraffin: HIF-2 alpha/EPAS1 Antibody [NB100-122] - Hif-2 alpha immunoreactivity in human cardiac myocytes stained with NB100-122.
	Biological Strategies Validation. Western Blot: HIF-2 alpha/EPAS1 Antibody [NB100-122] - Analysis on normoxic and hypoxic nuclear rat cell lysates.
	Western Blot: HIF-2 alpha/EPAS1 Antibody [NB100-122] - Western Blot on Human Glioma cells, whole cell lysates from a customer review.
	Simple Western: HIF-2 alpha/EPAS1 Antibody [NB100-122] - Simple Western lane view shows a specific band for HIF-2 alpha in 0.5 mg/ml of Hypoxic HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

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Background

Hypoxia-inducible factors (HIFs) generate heterodimers composed of alpha-subunits (HIF-1 alpha, HIF-2 alpha and HIF-3 alpha) and beta subunits (Arnt1, Arnt2 and Arnt3). Of these isoforms, HIF-1 alpha and HIF-2 alpha play critical roles in the cellular response to hypoxic conditions. After heterodimerization with beta subunits binding to DNA sequence 5'-[AG]CGTG-3' within HRE (hypoxia response element) of target gene promoters, HIF-2 alpha regulates the gene expression in response to oxygen homeostasis alterations. HIF-2 alpha regulates VEGF expression involved in the development of new blood vessels as well as the tubular system of the lung. HIF-2 alpha plays a role in the formation of the endothelium that gives rise to blood brain barrier. HIF-2 alpha is a strong activator of expression of Tie-2 tyrosine kinase, which is a major player in angiogenesis. HIF-2 alpha activation requires recruitment of transcriptional coactivators such as CREBPB, EP300, and its interaction with redox regulatory protein APEX mediates activation of CTAD. HIF-2 alpha and HIF-1 alpha expression increases in certain malignancies; wherein, HIF-1 alpha plays both pro- as well as anti-proliferative activities. HIF-2 alpha is suggested to exert anti-proliferative activity only.

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