抗MTH1抗体 | Anti-MTH1 Antibody

• 価格
• Image
• Background

価格

目次に戻る

Image

	Western Blot: MTH1 Antibody [NB100-109] - Analysis of MTH1 in 1. Ntera2, 2. MCF7 cell lysate, 3. A431 cell lysate and 4. COS7 cell lysate.
	Immunocytochemistry/Immunofluorescence: MTH1 Antibody [NB100-109] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-MTH1 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
	Western Blot: MTH1 Antibody [NB100-109] - MTH1 antibody was tested in the labeled lanes.
	Immunocytochemistry/Immunofluorescence: MTH1 Antibody [NB100-109] - Detection of MTH1 (Green) in Hela cells using NB100-109. Nuclei (Blue) are counterstained with Hoechst 33258.
	Immunocytochemistry/Immunofluorescence: MTH1 Antibody [NB100-109] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-MTH1 (NB100-109) at a 1:200 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
	Simple Western: MTH1 Antibody [NB100-109] - Simple Western lane view shows a specific band for MTH1 in 0.5 mg/ml of A431 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

目次に戻る

Background

MTH1 (human MutT Homolog 1) is an oxidized purine nucleoside triphosphatase enzyme that belongs to the Nudix hydrolase family and it acts as major mammalian detoxifier of the oxidized DNA precursor, 8-oxo-dGTP thereby exerting antimutagenic effects and suppressing cell dysfunction/death induced by oxidative stress. MTH1 hydrolyzes 8-oxo-dGTP, 8-oxo-dATP and 2-OH-dATP, thereby preventing misincorporation of oxidized purine nucleoside triphosphates into DNA and subsequently preventing A:T to C:G and G:C to T:A transversions. MTH1 also hydrolyzes the corresponding ribonucleotides, 2-OH-ATP, 8-oxo-GTP and 8-oxo-ATP. 2-OH-dADP, 8-OH-dGDP and 8-OH-dGTP inhibit the 2-OH-dATPase activity whereas 8-OH-dGTPase activity is inhibited by 8-OH-dGDP, 2-OH-dADP and 2-OH-dATP. Localized mainly in the cytoplasm, MTH1 has been found in nucleus and mitochondria also, and is widely expressed with highest expression in thymus, testis, embryo, proliferating blood lymphocytes etc. Oxidative DNA damage, e.g. 8-oxoG is known to accumulate both in nuclear and mitochondrial genomes during aging, carcinogenesis or various neurodegenerative diseases, such as Parkinson's disease, Alzheimer's disease or amyotrophic lateral sclerosis and MTH1 deficiency can increase susceptibility to these oxidative damage mediated dysfunctions.

目次に戻る