抗MTH1抗体 | Anti-MTH1 Antibody

掲載日情報:2018/07/09 現在Webページ番号:46246

世界最大級の抗体製品数を取り扱うNovus Biologicals社のMTH1に対する抗体(anti-MTH1 | antibody MTH1)です。Novus Biologicals社の抗体は数多くの学術論文で使用実績があります。

本製品は研究用です。研究用以外には使用できません。

価格表左の「文献」アイコンから、使用文献情報一覧が表示できます。

価格

[在庫・価格 :2024年06月17日 16時55分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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納期 文献数
Anti-MTH1, Rabbit-Poly
3~4週間 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 28
説明文
Simple Western対応抗体。抗原:C-terminal,Keywords:8-oxo-7|8-dihydrodeoxyguanosine triphosphatase|EC 3.6.1.-|MTH1Nucleoside diphosphate-linked moiety X motif 1|mutT human homolog 1|8-oxo-dGTPase|nucleoside diphosphate-linked moiety X-type motif 17|8-dihydro-8-oxoguanine triphosphatase
Genbank No: 4521
Protein Accession No: P36639
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Rabbit クラス IgG 標識 Unlabeled
交差性 Human/Mouse/Rat 適用 IC,IF,IHC,Immunoblotting,Simple Western,Western Blot
クロナリティ Polyclonal フォーマット 性状 Antigen Affinity Purified 吸収処理
掲載カタログ

製品記事 抗MTH1抗体 | Anti- MTH1 antibody
抗MTH1抗体
関連記事

[在庫・価格 :2024年06月17日 16時55分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。

Anti-MTH1, Rabbit-Poly

文献数: 28

説明文 Simple Western対応抗体。抗原:C-terminal,Keywords:8-oxo-7|8-dihydrodeoxyguanosine triphosphatase|EC 3.6.1.-|MTH1Nucleoside diphosphate-linked moiety X motif 1|mutT human homolog 1|8-oxo-dGTPase|nucleoside diphosphate-linked moiety X-type motif 17|8-dihydro-8-oxoguanine triphosphatase
Genbank No: 4521
Protein Accession No: P36639
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Rabbit
交差性 Human/Mouse/Rat 適用 IC,IF,IHC,Immunoblotting,Simple Western,Western Blot
標識 Unlabeled 性状 Antigen Affinity Purified
吸収処理 クラス IgG
クロナリティ Polyclonal フォーマット
掲載カタログ

製品記事 抗MTH1抗体 | Anti- MTH1 antibody
抗MTH1抗体
関連記事



目次に戻る

Image

Western Blot: MTH1 Antibody [NB100-109] - Analysis of MTH1 in 1. Ntera2, 2. MCF7 cell lysate, 3. A431 cell lysate and 4. COS7 cell lysate.
Immunocytochemistry/Immunofluorescence: MTH1 Antibody [NB100-109] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-MTH1 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Western Blot: MTH1 Antibody [NB100-109] - MTH1 antibody was tested in the labeled lanes.
Immunocytochemistry/Immunofluorescence: MTH1 Antibody [NB100-109] - Detection of MTH1 (Green) in Hela cells using NB100-109. Nuclei (Blue) are counterstained with Hoechst 33258.
Immunocytochemistry/Immunofluorescence: MTH1 Antibody [NB100-109] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-MTH1 (NB100-109) at a 1:200 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Simple Western: MTH1 Antibody [NB100-109] - Simple Western lane view shows a specific band for MTH1 in 0.5 mg/ml of A431 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.


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Background

MTH1 (human MutT Homolog 1) is an oxidized purine nucleoside triphosphatase enzyme that belongs to the Nudix hydrolase family and it acts as major mammalian detoxifier of the oxidized DNA precursor, 8-oxo-dGTP thereby exerting antimutagenic effects and suppressing cell dysfunction/death induced by oxidative stress. MTH1 hydrolyzes 8-oxo-dGTP, 8-oxo-dATP and 2-OH-dATP, thereby preventing misincorporation of oxidized purine nucleoside triphosphates into DNA and subsequently preventing A:T to C:G and G:C to T:A transversions. MTH1 also hydrolyzes the corresponding ribonucleotides, 2-OH-ATP, 8-oxo-GTP and 8-oxo-ATP. 2-OH-dADP, 8-OH-dGDP and 8-OH-dGTP inhibit the 2-OH-dATPase activity whereas 8-OH-dGTPase activity is inhibited by 8-OH-dGDP, 2-OH-dADP and 2-OH-dATP. Localized mainly in the cytoplasm, MTH1 has been found in nucleus and mitochondria also, and is widely expressed with highest expression in thymus, testis, embryo, proliferating blood lymphocytes etc. Oxidative DNA damage, e.g. 8-oxoG is known to accumulate both in nuclear and mitochondrial genomes during aging, carcinogenesis or various neurodegenerative diseases, such as Parkinson's disease, Alzheimer's disease or amyotrophic lateral sclerosis and MTH1 deficiency can increase susceptibility to these oxidative damage mediated dysfunctions.

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