抗Histone H3S10ph (phospho Ser10)抗体 | Anti-Histone H3S10ph (phospho Ser10) antibody
掲載日情報:2020/04/13 現在Webページ番号:45512
GeneTex社の抗Histone H3S10ph (phospho Ser10)抗体 | Anti-Histone H3S10ph (phospho Ser10) antibodyです。
※ 本製品は研究用です。研究用以外には使用できません。
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特長
- 高品質の抗体です。
- 幅広い研究分野に関連する抗体を取り揃えています。
- 使用されたアプリケーションや動物種などの情報が充実しています。
- 多数の論文で使用実績がある信頼性の高い抗体です。
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価格
[在庫・価格 :2025年04月26日 08時35分現在]
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Anti-Histone H3S10ph (pSer10), Phosphorylated, Rabbit |
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Anti-Histone H3S10ph (pSer10), Phosphorylated, Rabbit |
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[在庫・価格 :2025年04月26日 08時35分現在]
Anti-Histone H3S10ph (pSer10), Phosphorylated, Rabbit
文献数: 8
- 商品コード:GTX128116
- メーカー:GNT
- 包装:100μl
- 価格:¥85,000
- 在庫:1個
- 納期:10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 | レビューあり。 別名:H3/A antibody,H3FA antibody,HIST1H3B antibody,HIST1H3C antibody,HIST1H3D antibody,HIST1H3E antibody,HIST1H3F antibody,HIST1H3G antibody,HIST1H3H antibody,HIST1H3I antibody,HIST1H3J antibody,HIST1H3A antibody,histone H3/j antibody,histone H3/i antibody Genbank No: 8350 |
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別包装品 | 別包装品あり | ||||||
法規制等 | |||||||
保存条件 | -20℃ | 法規備考 | |||||
抗原種 | Human | 免疫動物 | Rabbit | ||||
交差性 | Bovine/Human/Mouse/Rat/Zebrafish | 適用 | ChIP,IC,IF,IHC,IP,Western Blot | ||||
標識 | Unlabeled | 性状 | Purified | ||||
吸収処理 | クラス | IgG | |||||
クロナリティ | Polyclonal | フォーマット | |||||
掲載カタログ |
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製品記事 | 抗ヒストン抗体/修飾ヒストン抗体 ゼブラフィッシュ(zebrafish)研究用製品特集 |
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関連記事 | GeneTex社における抗体の品質管理 |
Anti-Histone H3S10ph (pSer10), Phosphorylated, Rabbit
文献数: 8
- 商品コード:GTX128116
- メーカー:GNT
- 包装:25μl
- 価格:¥30,000
- 在庫:無(未発注)
- 納期:10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 | レビューあり。 別名:H3/A antibody,H3FA antibody,HIST1H3B antibody,HIST1H3C antibody,HIST1H3D antibody,HIST1H3E antibody,HIST1H3F antibody,HIST1H3G antibody,HIST1H3H antibody,HIST1H3I antibody,HIST1H3J antibody,HIST1H3A antibody,histone H3/j antibody,histone H3/i antibody Genbank No: 8350 |
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別包装品 | 別包装品あり | ||||||
法規制等 | |||||||
保存条件 | -20℃ | 法規備考 | |||||
抗原種 | Human | 免疫動物 | Rabbit | ||||
交差性 | Bovine/Human/Mouse/Rat/Zebrafish | 適用 | ChIP,IC,IF,IHC,IP,Western Blot | ||||
標識 | Unlabeled | 性状 | Purified | ||||
吸収処理 | クラス | IgG | |||||
クロナリティ | Polyclonal | フォーマット | |||||
掲載カタログ |
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製品記事 | 抗ヒストン抗体/修飾ヒストン抗体 ゼブラフィッシュ(zebrafish)研究用製品特集 使いっきり抗体 |
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関連記事 | GeneTex社における抗体の品質管理 |
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DATA IMAGES
![]() | ![]() Histone H3 (phospho Ser10) antibody detects HIST1H3A protein by western blot analysis. A. 30 μg U2OS whole cell lysate/extract (untreated) B. 30 μg U2OS whole cell lysate/extract (serum deprivation for 48hr) C. 30 μg U2OS whole cell lysate/extract (100ng/ml Nocodazole treatment for 24hr 15% SDS-PAGE Histone H3 (phospho Ser10) antibody (GTX128116) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
![]() | Histone H3S10ph (phospho Ser10) antibody detects Histone H3S10ph (phospho Ser10) protein on zebrafish by whole mount immunohistochemical analysis. Sample: Paraformaldehyde-fixed 2 day-post-fertilization zebrafish embryo.Histone H3S10ph (phospho Ser10) (GTX128116) antibody dilution: 1:100. |
![]() | ![]() Histone H3 (phospho Ser10) antibody detects HIST1H3A protein by western blot analysis. A. 30 μg 4T1 whole lysate/extract (untreated) B. 30 μg 4T1 whole cell lysate/extract (100ng/ml Nocodazole treatment for 24hr) 15% SDS-PAGE Histone H3 (phospho Ser10) antibody (GTX128116) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
![]() | Histone H3S10ph (phospho Ser10) antibody immunoprecipitates HIST1H3B protein in IP experiments. IP samples: 293T whole cell extract treat with 100ng/ml Nocodazole for 24 hr A. 30 μg 293T whole cell extract treat with 100ng/ml Nocodazole for 24 hr B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of HIST1H3B protein by 4 μg Histone H3S10ph (phospho Ser10) antibody (GTX128116) 15 % SDS-PAGE The immunoprecipitated HIST1H3B protein was detected by Histone H3S10ph (phospho Ser10) antibody (GTX128116) diluted at 1:1000. [EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent] |
![]() | Histone H3 (phospho Ser10) antibody detects HIST1H3A protein at nucleus on human gastric cancer by immunohistochemical analysis. Sample: Paraffin-embedded gastric cancer. Histone H3 (phospho Ser10) antibody (GTX128116) dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min |
![]() | Histone H3S10ph (phospho Ser10) antibody detects Histone H3S10ph (phospho Ser10) protein on zebrafish by whole mount immunohistochemical analysis. Sample: Paraformaldehyde-fixed 2 day-post-fertilization zebrafish embryo.Histone H3S10ph (phospho Ser10) antibody (GTX128116) dilution: 1:100. |
![]() | Cross-linked ChIP was performed with HeLa chromatin extract treated with Nocodazole (100 ng/ml for 24 h) and 5 μg of either control rabbit IgG or anti-Histone H3S10ph (phospho Ser10) antibody. The precipitated DNA was detected by PCR with primer set targeting to GAPDH promoter. |
![]() | HIST1H3A antibody histone cluster 1, H3a protein by immunofluorescent analysis. Sample: RFP-tag transfected HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: histone cluster 1, H3a protein stained by HIST1H3A antibody (GTX128116) diluted at 1:500. Red: Tubulin, alpha 1b protein stained by TUBA1B antibody (GTX11304) diluted at 1:500 . Blue: Hoechst 33342 staining. |
![]() | ![]() Untreated (–) and treated (+) HeLa whole cell extracts (5 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3S10ph (phospho Ser10) antibody (GTX128116) diluted at 1:2000. |
![]() | Histone H3 (phospho Ser10) antibody detects Histone H3 (phospho Ser10) protein at chromosome by confocal immunofluorescent analysis. Sample: Mitotic HeLa cells were fixed in 2% paraformaldehyde for 30 min. Green: Histone H3 (phospho Ser10) protein stained by Histone H3 (phospho Ser10) antibody (GTX128116) diluted at 1:500. Red: alpha Tubulin antibody (GTX11304) diluted at 1:2500. Blue: Hoechst 33342 staining. [Images captured by Olympus FV1000 Confocal Laser Scanning Microscope] |
![]() | ![]() Histone H3 (phospho Ser10) antibody detects Histone H3 (phospho Ser10) protein by western blot analysis. A. 30 μg Rat2 whole cell lysate/extract (untreated) B. 30 μg Rat2 whole cell lysate/extract (100 ng/ml Nocodazole treatment for 24 hr) 15% SDS-PAGE Histone H3 (phospho Ser10) antibody (GTX128116) dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
![]() | The ICC/IF analysis of Histone H3S10ph (phospho Ser10) antibody was published by Lin MJ and colleagues in the journal Sci Rep in 2016.PMID: 27819330 |
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製品情報
Host | Rabbit |
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Clonality | Polyclonal |
Isotype | IgG |
Application | WB, ICC/IF, IHC-P, IHC-Wm, IP, ChIP assay |
Reactivity | Human, Mouse, Rat, Zebrafish |
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APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.Application | Dilution |
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WB | 1:500-1:3000 |
ICC/IF | 1:100-1:1000 |
IHC-P | 1:100-1:1000 |
IHC-Wm | 1:100-1:500 |
IP | 1:100-1:500 |
ChIP assay | Assay dependent |
Calculated MW | 15 kDa. ( Note ) |
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Positive Control | HeLa , HeLa (100 ng/ml Nacodazole treatment for 24 hr) , U2OS(100ng/ml Nocodazole treatment for 24hr) , 4T1 (100ng/ml Nocodazole treatment for 24hr) , Rat2 (100 ng/ml Nocodazole treatment for 24 hr) |
Predict Reactivity | Chimpanzee(>80% identity) |
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PROPERTIES
Form | Liquid |
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Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
Concentration | 0.35 mg/ml (Please refer to the vial label for the specific concentration.) |
Antigen Species | Human |
Immunogen | Carrier-protein conjugated synthetic peptide corresponding to residues around human Histone H3S10ph (phospho Ser10). The exact sequence is proprietary. |
Purification | Purified by antigen-affinity chromatography. |
Conjugation | Unconjugated |
Note | For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption. |
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TARGET
Cellular Localization | Nucleus |
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REFERENCE
Application Reference | Application/Reactivity |
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Camacho LE et al. Theriogenology 2018; 116 : 43111 Effects of maternal nutrient restriction followed by realimentation during early and mid-gestation in beef cows. II. Placental development, umbilical blood flow, and uterine blood flow responses to diet-alterations. | Application : IHC-P Reactivity : |
Wang WD et al. Int J Mol Sci 2017; 18 (1) Retinoic Acid Protects and Rescues the Development of Zebrafish Embryonic Retinal Photoreceptor Cells from Exposure to Paclobutrazol. | Application : IHC-Wm Reactivity : Zebrafish |
Lin MJ et al. Sci Rep 2016; 6 : 36188 Stathmin-like 4 is critical for the maintenance of neural progenitor cells in dorsal midbrain of zebrafish larvae. | Application : IHC-Wm Reactivity : Zebrafish |
Santos M et al. Mol Cell Biochem 2015; 399 (43102) : 143-53 LAP1 is a crucial protein for the maintenance of the nuclear envelope structure and cell cycle progression. | Application : WB Reactivity : Human |
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