抗Histone H2A.XS139ph (phospho Ser139)抗体 | Anti-Histone H2A.XS139ph (phospho Ser139) antibody
掲載日情報:2020/04/13 現在Webページ番号:45371
GeneTex社の抗Histone H2A.XS139ph (phospho Ser139)抗体 | Anti-Histone H2A.XS139ph (phospho Ser139) antibodyです。
※ 本製品は研究用です。研究用以外には使用できません。
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⇒抗Histone H2A.XS139ph (phospho Ser139)抗体(#GTX127340)のユーザーレビューはこちら。
Rating | ![]() ![]() ![]() ![]() ![]() | ( Average 5 based on 1 users reviews) |
Application | ||
Western Blot(WB) | ![]() ![]() ![]() ![]() ![]() | ( Average 5 based on 1 users reviews) |
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特長
- 高品質の抗体です。
- 幅広い研究分野に関連する抗体を取り揃えています。
- 使用されたアプリケーションや動物種などの情報が充実しています。
- 多数の論文で使用実績がある信頼性の高い抗体です。
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価格
[在庫・価格 :2025年04月26日 08時35分現在]
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Anti-Histone H2A.XS139ph (pSer139), Phosphorylated, Rabbit |
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Anti-Histone H2A.XS139ph (pSer139), Phosphorylated, Rabbit |
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40 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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[在庫・価格 :2025年04月26日 08時35分現在]
Anti-Histone H2A.XS139ph (pSer139), Phosphorylated, Rabbit
文献数: 40
- 商品コード:GTX127340
- メーカー:GNT
- 包装:25μl
- 価格:¥30,000
- 在庫:無(未発注)
- 納期:10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 | レビューあり。 別名:H2A histone family member X,H2A.X,H2A/X,H2AX Genbank No: 3014 |
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別包装品 | 別包装品あり | ||||||
法規制等 | |||||||
保存条件 | -20℃ | 法規備考 | |||||
抗原種 | Human | 免疫動物 | Rabbit | ||||
交差性 | Human/Mouse/Rat/Zebrafish | 適用 | IC,IF,IHC,Western Blot | ||||
標識 | Unlabeled | 性状 | Purified | ||||
吸収処理 | クラス | IgG | |||||
クロナリティ | Polyclonal | フォーマット | |||||
掲載カタログ |
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製品記事 | 抗ヒストン抗体/修飾ヒストン抗体 抗ヒストンH2A抗体 ゼブラフィッシュ(zebrafish)研究用製品特集 使いっきり抗体 |
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関連記事 | GeneTex社における抗体の品質管理 |
Anti-Histone H2A.XS139ph (pSer139), Phosphorylated, Rabbit
文献数: 40
- 商品コード:GTX127340
- メーカー:GNT
- 包装:100μl
- 価格:¥85,000
- 在庫:1個
- 納期:10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 | レビューあり。 別名:H2A histone family member X,H2A.X,H2A/X,H2AX Genbank No: 3014 |
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別包装品 | 別包装品あり | ||||||
法規制等 | |||||||
保存条件 | -20℃ | 法規備考 | |||||
抗原種 | Human | 免疫動物 | Rabbit | ||||
交差性 | Human/Mouse/Rat/Zebrafish | 適用 | IC,IF,IHC,Western Blot | ||||
標識 | Unlabeled | 性状 | Purified | ||||
吸収処理 | クラス | IgG | |||||
クロナリティ | Polyclonal | フォーマット | |||||
掲載カタログ |
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製品記事 | 抗ヒストン抗体/修飾ヒストン抗体 抗ヒストンH2A抗体 ゼブラフィッシュ(zebrafish)研究用製品特集 |
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関連記事 | GeneTex社における抗体の品質管理 |
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DATA IMAGES
![]() | ![]() Untreated (–) and treated (+) HCT-116 whole cell extract (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2A.XS139ph (phospho Ser139) antibody (GTX127340) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
![]() | ![]() Histone H2A.X (phospho Ser139) antibody detects H2AFX protein in cisplatin-treated samples by western blot analysis. A. 30 μg NIH-3T3 whole cell lysate/extract (untreated) B. 30 μg NIH-3T3 whole cell lysate/extract (30μM cisplatin treatment for 24hr) 15% SDS-PAGE Histone H2A.X (phospho Ser139) antibody (GTX127340) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
![]() | ![]() Histone H2A.X (phospho Ser139) antibody detects H2AFX protein by western blot analysis. A. 30 μg HCT116 whole cell lysate/extract (untreated for 2hr) B. 30 μg HCT116 whole cell lysate/extract (UVB treatment 50mJ for 2hr) C. 30 μg HCT116 whole cell lysate/extract (untreated for 6hr) D. 30 μg HCT116 whole cell lysate/extract (UVB treatment 50mJ for 6hr) 15% SDS-PAGE Histone H2A.X (phospho Ser139) antibody (GTX127340) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
![]() | Histone H2A.XS139ph (phospho Ser139) antibody detects Histone H2A.XS139ph (phospho Ser139) protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human breast carcinoma.Histone H2A.XS139ph (phospho Ser139) stained by Histone H2A.XS139ph (phospho Ser139) antibody (GTX127340) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min |
![]() | ![]() Untreated (–) and treated (+) PC-12 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2A.XS139ph (phospho Ser139) antibody (GTX127340) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
![]() | ![]() Histone H2A.XS139ph (phospho Ser139) antibody detects Histone H2A.XS139ph (phospho Ser139) protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Histone H2A.XS139ph (phospho Ser139) stained by Histone H2A.XS139ph (phospho Ser139) antibody (GTX127340) diluted at 1:1000.Red: phalloidin, a cytoskeleton marker, diluted at 1:100.Scale bar= 10 μm. |
![]() | The WB analysis of Histone H2A.XS139ph (phospho Ser139) antibody was published by Chien JY and colleagues in the journal Cell Death Discov in 2016.PMID: 27551500 |
![]() | The WB analysis of Histone H2A.XS139ph (phospho Ser139) antibody was published by Chien JY and colleagues in the journal Cell Death Discov in 2016.PMID: 27551500 |
![]() | ![]() Untreated (–) and treated (+) HCT-116 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2A.XS139ph (phospho Ser139) antibody (GTX127340) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
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製品情報
Host | Rabbit |
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Clonality | Polyclonal |
Isotype | IgG |
Application | WB, ICC/IF, IHC-P, IHC-Wm |
Reactivity | Human, Mouse, Rat, Zebrafish |
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APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.Application | Dilution |
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WB | 1:500-1:3000 |
ICC/IF | 1:100-1:1000 |
IHC-P | 1:100-1:1000 |
IHC-Wm | Assay dependent |
Calculated MW | 15 kDa. ( Note ) |
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Positive Control | HCT116 , HCT116 (30 μM cisplatin treatment for 24 hr) , HCT116 (UVB treatment 50mJ for 2hr) , HCT116 (UVB treatment 50mJ for 6hr) , NIH-3T3 (30μM cisplatin treatment for 24hr) , PC-12 (30uM cisplatin treatment for 24hr) |
Predict Reactivity | Bovine, Rhesus Monkey(>80% identity) |
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PROPERTIES
Form | Liquid |
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Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
Concentration | 0.16 mg/ml (Please refer to the vial label for the specific concentration.) |
Antigen Species | Human |
Immunogen | Carrier-protein conjugated synthetic peptide corresponding to residues around human Histone H2A.XS139ph (phospho Ser139). The exact sequence is proprietary. |
Purification | Purified by antigen-affinity chromatography. |
Conjugation | Unconjugated |
Note | For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption. |
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TARGET
Synonyms | H2A histone family member X , H2A.X , H2A/X , H2AX |
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Cellular Localization | Nucleus |
Background | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq] |
Database | ・ Gene ID: 3014 H2AFX ・ UniProt: P16104 H2AFX |
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REFERENCE
Application Reference | Application/Reactivity |
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Wang YY et al. Biochem Pharmacol 2020; 171 : 113713 Orabase-formulated gentian violet effectively improved oral potentially malignant disorder in vitro and in vivo. | Application : WB Reactivity : Human |
van Gastel J et al. Aging (Albany NY) 2019; 11 (23) : 11268-11313 The RXFP3 receptor is functionally associated with cellular responses to oxidative stress and DNA damage. | Application : WB Reactivity : Human |
Gizak A et al. Cell Death Dis 2019; 10 (10) : 712 Targeting a moonlighting function of aldolase induces apoptosis in cancer cells. | Application : ICC/IF Reactivity : Mouse |
Novoa B et al. Aging Cell 2019; : e13020 Rag1 immunodeficiency-induced early aging and senescence in zebrafish are dependent on chronic inflammation and oxidative stress. | Application : ICC/IF Reactivity : Zebrafish |
Lan Y et al. Br J Dermatol 2019; OPN3 is a key regulator of UVA-induced photoaging in human dermal fibroblast cells. | Application : WB, ICC/IF Reactivity : Human |
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