抗TET1 [N1], N-term抗体 | Anti-TET1 [N1], N-term antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET
• REFERENCEE(抜粋)

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	TET1 antibody detects TET1 protein by western blot analysis. Non-transfected (-) and mouse TET1-transfected (+, including DDDDK-tag) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with TET1 antibody (GTX125888) at a dilution of 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
	Western blot analysis of 293T cell are mock transfected (lane1) or with tagged-mTet1 (lane 2) for 24hrs, using either GTX125888 (diluted at 1:2000) (A) or anti-tag (B) antibody. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
	TET1 antibody [N1], N-term detects TET1 protein at nucleus by immunofluorescent analysis.Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: TET1 protein stained by TET1 antibody [N1], N-term (GTX125888) diluted at 1:500.Blue: Hoechst 33342 staining.

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製品情報

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, ChIP assay
Reactivity	Human, Mouse

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:500-1:20000
ICC/IF	1:100-1:1000
IHC-P	Assay dependent
ChIP assay	Assay dependent

Not tested in other applications.

Calculated MW	219 kDa. ( Note )
Positive Control	tagged-mTet1 , DDDDK-mouse TET1-transfected 293T
Specificity/Sensitivity	Knockdown/Knockout validation was supported by references (PMID: 29513657)

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PROPERTIES

Form	Liquid
Buffer	1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Mouse
Immunogen	Recombinant protein encompassing a sequence within the N-terminus region of mouse TET1. The exact sequence is proprietary.
Purification	Purified by antigen-affinity chromatography.
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	tet methylcytosine dioxygenase 1 , 2510010B09Rik , AA517754 , BB001228 , Cxxc6 , D10Ertd17e , LCX , mKIAA1676
Cellular Localization	Nucleus
Background	Dioxygenase that specifically binds methylcytosine (5mC), a minor base in mammalian DNA found in repetitive DNA elements that is crucial for retrotransposon silencing and mammalian development. Catalyzes the conversion of methylcytosine (5mC) to 5-hydroxymethylcytosine (hmC). The clear function of 5-hydroxymethylcytosine (hmC) is still unclear but it may influence chromatin structure and recruit specific factors or may constitute an intermediate component in cytosine demethylation. 5-hydroxymethylcytosine (hmC) is present in ES cells and is enriched in the brain, especially in Purkinje neurons. May play a role in the fetal development of heart, lung and brain.
Database	・ Gene ID: 52463 Tet1 ・ UniProt: Q3URK3 Tet1

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REFERENCEE(抜粋)

Application Reference	Application/Reactivity
Bai M et al. Oncogene 2018; (Epub) * sensitizes endometrial cancer cells to chemotherapy through idh1-induced nrf2 expression via an epigenetic mechanism.	Application : IHC-P Reactivity : Human
Chrysanthou S et al. Stem Cell Reports 2018; 10 (4) : 1355-1368 A Critical Role of TET1/2 Proteins in Cell-Cycle Progression of Trophoblast Stem Cells.	Application : ICC/IF, ChIP assay Reactivity : Mouse
Hill PWS et al. Nature 2018; 555 (7696) : 392-396 Epigenetic reprogramming enables the transition from primordial germ cell to gonocyte.	Application : WB Reactivity : Mouse
Coluccio A et al. Epigenetics Chromatin 2018; 11 (1) : 7 Individual retrotransposon integrants are differentially controlled by KZFP/KAP1-dependent histone methylation, DNA methylation and TET-mediated hydroxymethylation in naive embryonic stem cells.	Application : ChIP assay Reactivity : Mouse
Cui Y et al. Oncotarget 2016; 7 (40) : 64932-64941 miR-29 regulates Tet1 expression and contributes to early differentiation of mouse ESCs.	Application : WB Reactivity : Mouse

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