抗TET1 [N3C1]抗体 | Anti-TET1 [N3C1] antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET
• REFERENCEE(抜粋)

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

目次に戻る

価格

目次に戻る

DATA IMAGES

	Non-transfected (–) and transfected (+) 293T whole cell extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with TET1 antibody [N3C1] (GTX124207) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
	Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with TET1 antibody [N3C1] (GTX124207) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
	TET1 antibody [N3C1] detects TET1 protein at nucleus in human A549 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded human A549 xenograft . TET1 antibody [N3C1] (GTX124207) diluted at 1:250. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
	TET1 antibody [N3C1] detects TET1 protein at nucleus on Human normal prostate tissue by immunohistochemical analysis. Sample: Paraffin-embedded Human normal prostate tissue. TET1 antibody [N3C1] (GTX124207) dilution: 1:1000. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
	HeLa whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with TET1 antibody [N3C1] (GTX124207) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
	TET1 antibody [N3C1] detects TET1 protein at nucleus by immunofluorescent analysis.Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: TET1 stained by TET1 antibody [N3C1] (GTX124207) diluted at 1:1000.Blue: Hoechst 33342 staining.
	TET1 antibody [N3C1] detects TET1 protein by western blot analysis.A. 30 μg 293T whole cell lysate/extractB. 30 μg whole cell lysate/extract of DDDDK-human TET1-transfected 293T cells5% SDS-PAGETET1 antibody [N3C1] (GTX124207) dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
	Immunohistochemical analysis of paraffin-embedded Hela xenograft, using TET1 (GTX124207) antibody at 1:1000 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

目次に戻る

製品情報

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IP, ChIP assay, IHC
Reactivity	Human, Mouse, Monkey

目次に戻る

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IP	Assay dependent
ChIP assay	Assay dependent
IHC	Assay dependent

Not tested in other applications.

Calculated MW	235 kDa. ( Note )
Positive Control	293T , A431 , HeLa , HepG2 , 293T tagged-hTET1 , DDDDK-human TET1-transfected 293T

目次に戻る

PROPERTIES

Form	Liquid
Buffer	1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	3.66 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	Recombinant protein encompassing a sequence within the center region of human TET1. The exact sequence is proprietary.
Purification	Purified by antigen-affinity chromatography.
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

目次に戻る

TARGET

Synonyms	tet methylcytosine dioxygenase 1 , CXXC6 , LCX , bA119F7.1
Cellular Localization	Nucleus
Background	Dioxygenase that specifically binds methylcytosine (5mC), a minor base in mammalian DNA found in repetitive DNA elements that is crucial for retrotransposon silencing and mammalian development. Catalyzes the conversion of methylcytosine (5mC) to 5-hydroxymethylcytosine (hmC). The clear function of 5-hydroxymethylcytosine (hmC) is still unclear but it may influence chromatin structure and recruit specific factors or may constitute an intermediate component in cytosine demethylation. 5-hydroxymethylcytosine (hmC) is present in ES cells and is enriched in the brain, especially in Purkinje neurons. May play a role in the fetal development of heart, lung and brain.
Database	・ Gene ID: 80312 TET1 ・ UniProt: Q8NFU7 TET1

目次に戻る

REFERENCEE(抜粋)

Application Reference	Application/Reactivity
Wu J et al. J Exp Clin Cancer Res 2019; 38 (1) : 348 TET1-mediated DNA hydroxymethylation activates inhibitors of the Wnt/帣-catenin signaling pathway to suppress EMT in pancreatic tumor cells.	Application : WB Reactivity : Human
Volnitskiy A et al. PLoS One 2019; 14 (2) : e0211980 Abnormal activity of transcription factors gli in high-grade gliomas.	Application : WB Reactivity : Human
Pei YF et al. Am J Pathol 2019; (Epub) Methyl-CpG Binding Domain Protein 2 Inhibits the Malignant Characteristic of Lung Adenocarcinoma through the Epigenetic Modulation of 10 to 11 Translocation 1 and miR-200s.	Application : WB, IHC-P Reactivity : Human
Ciccarone F et al. Mol Nutr Food Res 2019; 63 (4) : e1800970 High Dietary Fat Intake Affects DNA Methylation/Hydroxymethylation in Mouse Heart: Epigenetic Hints for Obesity-Related Cardiac Dysfunction.	Application : WB Reactivity : Mouse
Ma F et al. J Cell Physiol 2018; (Epub) LIN28A activates the transcription of NANOG in dairy goat male germline stem cells.	Application : ICC/IF Reactivity : Human

目次に戻る