抗HDAC3抗体 | Anti-HDAC3 antibody
掲載日情報:2020/04/13 現在Webページ番号:40787
GeneTex社の抗HDAC3抗体 | Anti-HDAC3 antibodyです。
※ 本製品は研究用です。研究用以外には使用できません。
GeneTex社 ホームページでユーザーレビューが閲覧できます
GeneTex社では製品を使用したユーザーからのレビューを各国から集め、GeneTex社ホームページで公開しています。各ユーザーの使用アプリケーション、試料の由来動物、希釈倍率、ブロッキング試薬といった実験条件, 実験結果画像など参考となる情報が掲載されています。
⇒抗HDAC3抗体(#GTX113303)のユーザーレビューはこちら。
Rating | ( Average 5 based on 1 users reviews) | |
Application | ||
Western Blot(WB) | ( Average 5 based on 1 users reviews) |
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特長
- 高品質の抗体です。
- 幅広い研究分野に関連する抗体を取り揃えています。
- 使用されたアプリケーションや動物種などの情報が充実しています。
- 多数の論文で使用実績がある信頼性の高い抗体です。
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価格
[在庫・価格 :2024年03月28日 17時15分現在]
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Anti-HDAC3, Rabbit |
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[在庫・価格 :2024年03月28日 17時15分現在]
Anti-HDAC3, Rabbit
文献数: 6
- 商品コード:GTX113303
- メーカー:GNT
- 包装:100μl
- 価格:¥76,000
- 在庫:1個
- 納期:10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 |
レビューあり。KO/KDバリデーション済み抗体。 別名:histone deacetylase 3,HD3,KDAC3,RPD3,RPD3-2 Genbank No: 8841 |
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別包装品 | 別包装品あり | ||||||
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保存条件 | -20℃ | 法規備考 | |||||
抗原種 | Human | 免疫動物 | Rabbit | ||||
交差性 | Human/Mouse/Rat/Zebrafish | 適用 | ChIP,IC,IF,IHC,IP,Western Blot | ||||
標識 | Unlabeled | 性状 | Purified | ||||
吸収処理 | クラス | IgG | |||||
クロナリティ | Polyclonal | フォーマット | |||||
掲載カタログ |
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製品記事 | |||||||
関連記事 |
GeneTex社における抗体の品質管理 |
Anti-HDAC3, Rabbit
文献数: 6
- 商品コード:GTX113303
- メーカー:GNT
- 包装:25μl
- 価格:¥23,000
- 在庫:無(未発注)
- 納期:10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 |
レビューあり。KO/KDバリデーション済み抗体。 別名:histone deacetylase 3,HD3,KDAC3,RPD3,RPD3-2 Genbank No: 8841 |
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別包装品 | 別包装品あり | ||||||
法規制等 | |||||||
保存条件 | -20℃ | 法規備考 | |||||
抗原種 | Human | 免疫動物 | Rabbit | ||||
交差性 | Human/Mouse/Rat/Zebrafish | 適用 | ChIP,IC,IF,IHC,IP,Western Blot | ||||
標識 | Unlabeled | 性状 | Purified | ||||
吸収処理 | クラス | IgG | |||||
クロナリティ | Polyclonal | フォーマット | |||||
掲載カタログ |
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製品記事 |
使いっきり抗体 |
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関連記事 |
GeneTex社における抗体の品質管理 |
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DATA IMAGES
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody (GTX113303) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. | |
HDAC3 antibody immunoprecipitates HDAC3 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. 40 μg 293T whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of HDAC3 protein by 2 μg of HDAC3 antibody (GTX113303) 7.5% SDS-PAGE The immunoprecipitated HDAC3 protein was detected by HDAC3 antibody (GTX113303) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent. | |
Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using HDAC3(GTX113303) antibody at 1:250 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min | |
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody (GTX113303) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. | |
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody (GTX113303) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. | |
Cross-linked ChIP was performed with MCF-7 chromatin extract and 5 μg of either control rabbit IgG or anti-HDAC3 antibody. The precipitated DNA was detected by PCR with primer set targeting to TFF1 or HK1. ChIP experiment and primer designs are based on Proc Natl Acad Sci U S A. 2005 Aug 16;102(33):11651-6. | |
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody (GTX113303) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. | |
HDAC3 antibody detects HDAC3 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HDAC3 protein stained by HDAC3 antibody (GTX113303) diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Hoechst 33342 staining. | |
HDAC3 antibody detects HDAC3 protein by Western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody (GTX113303) diluted by 1:1000. | |
The WB, IP analysis of HDAC3 antibody was published by Armour SM and colleagues in the journal Nat Commun in 2017.PMID: 28916805 |
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製品情報
Host | Rabbit |
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Clonality | Polyclonal |
Isotype | IgG |
Application | WB, ICC/IF, IHC-P, IP, ChIP assay |
Reactivity | Human, Mouse, Rat |
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APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.Application | Dilution |
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WB | 1:500-1:3000 |
ICC/IF | 1:100-1:1000 |
IHC-P | 1:100-1:1000 |
IP | 1:100-1:500 |
ChIP assay | Assay dependent |
Calculated MW | 49 kDa. ( Note ) |
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Positive Control | 293T , A431 , HeLa , HepG2 , U87-MG , SK-N-SH , IMR32 , SK-N-AS , Neuro2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat2 , *NB2a |
Predict Reactivity | Zebrafish, Bovine, Chicken, Pig, Rhesus Monkey(>80% identity) |
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PROPERTIES
Form | Liquid |
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Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
Concentration | 0.32 mg/ml (Please refer to the vial label for the specific concentration.) |
Antigen Species | Human |
Immunogen | Recombinant protein encompassing a sequence within the center region of human HDAC3. The exact sequence is proprietary. |
Purification | Purified by antigen-affinity chromatography. |
Conjugation | Unconjugated |
Note | For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption. |
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TARGET
Synonyms | histone deacetylase 3 , HD3 , KDAC3 , RPD3 , RPD3-2 |
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Cellular Localization | Nucleus |
Background | Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. This gene is regarded as a potential tumor suppressor gene. [provided by RefSeq] |
Database | ・ Gene ID: 8841 HDAC3 ・ UniProt: O15379 HDAC3 |
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REFERENCE
Application Reference | Application/Reactivity |
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Miscianinov V. et al. Molecular Therapy 2018; (Epub) MicroRNA-148b targets the TGF-β pathway to regulate angiogenesis and endothelialto-mesenchymal transition during skin wound healing. | Application : WB Reactivity : Human |
Armour SM et al. Nat Commun 2017; 8 (1) : 549 An HDAC3-PROX1 corepressor module acts on HNF4α to control hepatic triglycerides. | Application : WB Reactivity : Human |
Emmett MJ et al. Nature 2017; (Epub) Histone deacetylase 3 prepares brown adipose tissue for acute thermogenic challenge. | Application : WB Reactivity : Mouse |
Soriano FX et al. PLoS One 2011; 6 (6) : e21056 In cortical neurons HDAC3 activity suppresses RD4-dependent SMRT export. | Application : ICC/IF Reactivity : Rat |
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