抗TNF alpha抗体 | Anti-TNF alpha antibody
掲載日情報:2020/04/13 現在Webページ番号:39635
GeneTex社の抗TNF alpha抗体 | Anti-TNF alpha antibodyです。
※ 本製品は研究用です。研究用以外には使用できません。
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特長
- 高品質の抗体です。
- 幅広い研究分野に関連する抗体を取り揃えています。
- 使用されたアプリケーションや動物種などの情報が充実しています。
- 多数の論文で使用実績がある信頼性の高い抗体です。
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価格
[在庫・価格 :2025年04月29日 20時55分現在]
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Anti-TNF alpha, Rabbit |
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Anti-TNF alpha, Rabbit |
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[在庫・価格 :2025年04月29日 20時55分現在]
Anti-TNF alpha, Rabbit
文献数: 81
- 商品コード:GTX110520
- メーカー:GNT
- 包装:100μl
- 価格:¥85,000
- 在庫:1個
- 納期:10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 | レビューあり。 別名:tumor necrosis factor,DIF,TNF-alpha,TNFA,TNFSF2,TNLG1F Genbank No: 7124 |
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別包装品 | 別包装品あり | ||||||
法規制等 | |||||||
保存条件 | -20℃ | 法規備考 | |||||
抗原種 | Human | 免疫動物 | Rabbit | ||||
交差性 | Bovine/Human/Mouse/Rat | 適用 | IC,IF,IHC,Western Blot | ||||
標識 | Unlabeled | 性状 | Purified | ||||
吸収処理 | クラス | IgG | |||||
クロナリティ | Polyclonal | フォーマット | |||||
掲載カタログ |
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製品記事 | 免疫チェックポイント関連抗体 |
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関連記事 | GeneTex社における抗体の品質管理 |
Anti-TNF alpha, Rabbit
文献数: 81
- 商品コード:GTX110520
- メーカー:GNT
- 包装:25μl
- 価格:¥30,000
- 在庫:1個
- 納期:10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 | レビューあり。 別名:tumor necrosis factor,DIF,TNF-alpha,TNFA,TNFSF2,TNLG1F Genbank No: 7124 |
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別包装品 | 別包装品あり | ||||||
法規制等 | |||||||
保存条件 | -20℃ | 法規備考 | |||||
抗原種 | Human | 免疫動物 | Rabbit | ||||
交差性 | Bovine/Human/Mouse/Rat | 適用 | IC,IF,IHC,Western Blot | ||||
標識 | Unlabeled | 性状 | Purified | ||||
吸収処理 | クラス | IgG | |||||
クロナリティ | Polyclonal | フォーマット | |||||
掲載カタログ |
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製品記事 | 使いっきり抗体 |
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関連記事 | GeneTex社における抗体の品質管理 |
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DATA IMAGES
![]() | TNF alpha antibody detects TNF alpha protein at cell membrane in mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. TNF alpha antibody (GTX110520) diluted at 1:400. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min |
![]() | TNF alpha antibody detects TNF alpha protein by western blot analysis. Whole cell extracts (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with TNF alpha antibody (GTX110520) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
![]() | TNF alpha antibody detects TNF alpha protein at cell membrane in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. TNF alpha antibody (GTX110520) diluted at 1:400. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min |
![]() | TNF alpha antibody detects TNF alpha protein at cytoplasm on human esophagus carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human esophagus carcinoma . TNF alpha antibody (GTX110520) diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min |
![]() | Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with TNF alpha antibody (GTX110520) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
![]() | TNF alpha antibody detects TNF alpha protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with TNF alpha antibody (GTX110520) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
![]() | TNF alpha antibody detects TNF alpha protein by western blot analysis. A. 30 μg NCI-H929 whole cell lysate/extract 12% SDS-PAGE TNF alpha antibody (GTX110520) dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
![]() | ![]() Untreated (–) and treated (+) Raw264.7 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with TNF alpha antibody (GTX110520) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
![]() | ![]() Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with TNF alpha antibody (GTX110520) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
![]() | ![]() The WB analysis of TNF alpha antibody was published by Lee AS and colleagues in the journal Cardiovasc Diabetol in 2014.PMID: 24666525 |
![]() | TNF alpha antibody detects TNF alpha protein at cytoplasm by immunofluorescent analysis. Sample: Raw264.7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TNF alpha protein stained by TNF alpha antibody (GTX110520) diluted at 1:500. Blue: Hoechst 33342 staining. |
![]() | TNF alpha antibody detects TNF alpha protein at membrane on human gastric carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human gastric carcinoma. TNF alpha antibody (GTX110520) dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min |
![]() | TNF alpha antibody detects TNF alpha protein at membrane by immunofluorescent analysis. Sample: jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TNF alpha protein stained by TNF alpha antibody (GTX110520) diluted at 1:500. Blue: Hoechst 33342 staining. |
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製品情報
Host | Rabbit |
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Clonality | Polyclonal |
Isotype | IgG |
Application | WB, ICC/IF, IHC-P |
Reactivity | Human, Mouse, Rat, Bovine |
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APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.Application | Dilution |
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WB | 1:500-1:3000 |
ICC/IF | 1:100-1:1000 |
IHC-P | 1:100-1:1000 |
Calculated MW | 26 kDa. ( Note ) |
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Positive Control | Raji , NCI-H929 , C8D30 , PC-12 , Rat-2 , Raw264.7 (50 ng/ml LPS treatment for 4 hr) , TNF-transfected 293T |
Predict Reactivity | Cat, Dog, Pig, Chimpanzee, Rhesus Monkey(>80% identity) |
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PROPERTIES
Form | Liquid |
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Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
Concentration | 0.04 mg/ml (Please refer to the vial label for the specific concentration.) |
Antigen Species | Human |
Immunogen | Full length human TNF alpha Recombinant protein. |
Purification | Purified by antigen-affinity chromatography. |
Conjugation | Unconjugated |
Note | For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption. |
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TARGET
Synonyms | tumor necrosis factor , DIF , TNF-alpha , TNFA , TNFSF2 , TNLG1F |
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Cellular Localization | Cell membrane; Single-pass type II membrane protein , Tumor necrosis factor , soluble form: Secreted |
Background | This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq] |
Database | ・ Gene ID: 7124 TNF ・ UniProt: P01375 TNF |
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REFERENCE
Application Reference | Application/Reactivity |
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Esposito E et al. Nat Commun 2019; 10 (1) : 5306 Brain-to-cervical lymph node signaling after stroke. | Application : WB Reactivity : Rat |
Thirugnanam K et al. J Am Heart Assoc 2019; 8 (22) : e012792 Cardiomyocyte-Specific Snrk Prevents Inflammation in the Heart. | Application : WB Reactivity : Mouse |
Huang TW et al. Neuropeptides 2019; 78 : 101964 Neuropeptide Y increases differentiation of human olfactory receptor neurons through the Y1 receptor. | Application : IHC-P Reactivity : Human |
Hsiao HT et al. J Biomed Sci 2019; 26 (1) : 74 The analgesic effect of propofol associated with the inhibition of hypoxia inducible factor and inflammasome in complex regional pain syndrome. | Application : WB Reactivity : Mouse |
Liu J et al. JBMR Plus 2019; 3 (5) : e10122 Identification of Genes Differentially Expressed in Simvastatin-Induced Alveolar Bone Formation. |
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