抗TNF alpha抗体 | Anti-TNF alpha antibody

掲載日情報:2020/04/13 現在Webページ番号:39635

GeneTex社の抗TNF alpha抗体 | Anti-TNF alpha antibodyです。

本製品は研究用です。研究用以外には使用できません。

特長

  • 高品質の抗体です。
  • 幅広い研究分野に関連する抗体を取り揃えています。
  • 使用されたアプリケーションや動物種などの情報が充実しています。
  • 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

[在庫・価格 :2025年04月29日 20時55分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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納期 文献数
Anti-TNF alpha, Rabbit
10日程度 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 81
説明文
レビューあり
別名:tumor necrosis factor,DIF,TNF-alpha,TNFA,TNFSF2,TNLG1F
Genbank No: 7124
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Rabbit クラス IgG 標識 Unlabeled
交差性 Bovine/Human/Mouse/Rat 適用 IC,IF,IHC,Western Blot
クロナリティ Polyclonal フォーマット 性状 Purified 吸収処理
掲載カタログ

製品記事 免疫チェックポイント関連抗体
関連記事 GeneTex社における抗体の品質管理
Anti-TNF alpha, Rabbit
10日程度 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 81
説明文
レビューあり
別名:tumor necrosis factor,DIF,TNF-alpha,TNFA,TNFSF2,TNLG1F
Genbank No: 7124
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Rabbit クラス IgG 標識 Unlabeled
交差性 Bovine/Human/Mouse/Rat 適用 IC,IF,IHC,Western Blot
クロナリティ Polyclonal フォーマット 性状 Purified 吸収処理
掲載カタログ

製品記事 使いっきり抗体
関連記事 GeneTex社における抗体の品質管理

[在庫・価格 :2025年04月29日 20時55分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。

Anti-TNF alpha, Rabbit

文献数: 81

説明文 レビューあり
別名:tumor necrosis factor,DIF,TNF-alpha,TNFA,TNFSF2,TNLG1F
Genbank No: 7124
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Rabbit
交差性 Bovine/Human/Mouse/Rat 適用 IC,IF,IHC,Western Blot
標識 Unlabeled 性状 Purified
吸収処理 クラス IgG
クロナリティ Polyclonal フォーマット
掲載カタログ

製品記事 免疫チェックポイント関連抗体
関連記事 GeneTex社における抗体の品質管理

Anti-TNF alpha, Rabbit

文献数: 81

説明文 レビューあり
別名:tumor necrosis factor,DIF,TNF-alpha,TNFA,TNFSF2,TNLG1F
Genbank No: 7124
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Rabbit
交差性 Bovine/Human/Mouse/Rat 適用 IC,IF,IHC,Western Blot
標識 Unlabeled 性状 Purified
吸収処理 クラス IgG
クロナリティ Polyclonal フォーマット
掲載カタログ

製品記事 使いっきり抗体
関連記事 GeneTex社における抗体の品質管理



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DATA IMAGES

TNF alpha antibody detects TNF alpha protein at cell membrane in mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
TNF alpha antibody (GTX110520) diluted at 1:400.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
TNF alpha antibody detects TNF alpha protein by western blot analysis. Whole cell extracts (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with TNF alpha antibody (GTX110520) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
TNF alpha antibody detects TNF alpha protein at cell membrane in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
TNF alpha antibody (GTX110520) diluted at 1:400.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
TNF alpha antibody detects TNF alpha protein at cytoplasm on human esophagus carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human esophagus carcinoma .
TNF alpha antibody (GTX110520) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with TNF alpha antibody (GTX110520) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
TNF alpha antibody detects TNF alpha protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with TNF alpha antibody (GTX110520) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
TNF alpha antibody detects TNF alpha protein by western blot analysis.
A. 30 μg NCI-H929 whole cell lysate/extract
12% SDS-PAGE
TNF alpha antibody (GTX110520) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Untreated (–) and treated (+) Raw264.7 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with TNF alpha antibody (GTX110520) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with TNF alpha antibody (GTX110520) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

The WB analysis of TNF alpha antibody was published by Lee AS and colleagues in the journal Cardiovasc Diabetol in 2014.PMID: 24666525
TNF alpha antibody detects TNF alpha protein at cytoplasm by immunofluorescent analysis.
Sample: Raw264.7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TNF alpha protein stained by TNF alpha antibody (GTX110520) diluted at 1:500.
Blue: Hoechst 33342 staining.
TNF alpha antibody detects TNF alpha protein at membrane on human gastric carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human gastric carcinoma.
TNF alpha antibody (GTX110520) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
TNF alpha antibody detects TNF alpha protein at membrane by immunofluorescent analysis.
Sample: jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TNF alpha protein stained by TNF alpha antibody (GTX110520) diluted at 1:500.
Blue: Hoechst 33342 staining.


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製品情報

HostRabbit
ClonalityPolyclonal
IsotypeIgG
ApplicationWB, ICC/IF, IHC-P
ReactivityHuman, Mouse, Rat, Bovine


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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW 26 kDa. ( Note )
Positive Control Raji , NCI-H929 , C8D30 , PC-12 , Rat-2 , Raw264.7 (50 ng/ml LPS treatment for 4 hr) , TNF-transfected 293T
Predict Reactivity Cat, Dog, Pig, Chimpanzee, Rhesus Monkey(>80% identity)


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PROPERTIES

Form Liquid
Buffer 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Storage Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration 0.04 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species Human
Immunogen Full length human TNF alpha Recombinant protein.
Purification Purified by antigen-affinity chromatography.
Conjugation Unconjugated
Note For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.


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TARGET

Synonyms tumor necrosis factor , DIF , TNF-alpha , TNFA , TNFSF2 , TNLG1F
Cellular Localization Cell membrane; Single-pass type II membrane protein , Tumor necrosis factor , soluble form: Secreted
Background This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq]
Database ・ Gene ID: 7124 TNF
・ UniProt: P01375 TNF


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REFERENCE

Application ReferenceApplication/Reactivity
Esposito E et al. Nat Commun 2019; 10 (1) : 5306 Brain-to-cervical lymph node signaling after stroke.Application : WB
Reactivity : Rat
Thirugnanam K et al. J Am Heart Assoc 2019; 8 (22) : e012792 Cardiomyocyte-Specific Snrk Prevents Inflammation in the Heart.Application : WB
Reactivity : Mouse
Huang TW et al. Neuropeptides 2019; 78 : 101964 Neuropeptide Y increases differentiation of human olfactory receptor neurons through the Y1 receptor.Application : IHC-P
Reactivity : Human
Hsiao HT et al. J Biomed Sci 2019; 26 (1) : 74 The analgesic effect of propofol associated with the inhibition of hypoxia inducible factor and inflammasome in complex regional pain syndrome.Application : WB
Reactivity : Mouse
Liu J et al. JBMR Plus 2019; 3 (5) : e10122 Identification of Genes Differentially Expressed in Simvastatin-Induced Alveolar Bone Formation.


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