抗beta Actin抗体 | Anti-beta Actin antibody
掲載日情報:2020/04/13 現在Webページ番号:39264
GeneTex社の抗beta Actin抗体 | Anti-beta Actin antibodyです。
※ 本製品は研究用です。研究用以外には使用できません。
GeneTex社 ホームページでユーザーレビューが閲覧できます
GeneTex社では製品を使用したユーザーからのレビューを各国から集め、GeneTex社ホームページで公開しています。各ユーザーの使用アプリケーション、試料の由来動物、希釈倍率、ブロッキング試薬といった実験条件, 実験結果画像など参考となる情報が掲載されています。
⇒抗beta Actin抗体(#GTX109639)のユーザーレビューはこちら。
Rating | ![]() ![]() ![]() ![]() ![]() | ( Average 4.7 based on 11 users reviews) |
Application | ||
Western Blot(WB) | ![]() ![]() ![]() ![]() ![]() | ( Average 4.7 based on 11 users reviews) |
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特長
- 高品質の抗体です。
- 幅広い研究分野に関連する抗体を取り揃えています。
- 使用されたアプリケーションや動物種などの情報が充実しています。
- 多数の論文で使用実績がある信頼性の高い抗体です。
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価格
[在庫・価格 :2025年05月15日 00時00分現在]
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Anti-β-Actin, Rabbit-Poly <Anti-ACTB> |
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Anti-β-Actin, Rabbit-Poly <Anti-ACTB> |
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614 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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[在庫・価格 :2025年05月15日 00時00分現在]
Anti-β-Actin, Rabbit-Poly <Anti-ACTB>
文献数: 614
- 商品コード:GTX109639
- メーカー:GNT
- 包装:25μl
- 価格:¥30,000
- 在庫:1個
- 納期:10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 | レビューあり。KO/KDバリデーション済み抗体。抗原:aa 359~368 別名:actin beta,BRWS1,PS1TP5BP1 Genbank No: 60 |
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別包装品 | 別包装品あり | ||||||
法規制等 | |||||||
保存条件 | -20℃ | 法規備考 | |||||
抗原種 | Human | 免疫動物 | Rabbit | ||||
交差性 | Caenorhabditis elegans/Candida albicans/Cat/Chicken/Dog/Drosophila/E. coli/Hamster/Horse/Human/Monkey/Mosquito/Mouse/Pig/Plant/Rat/Rice/Sheep/Treeshrew/Worm/Xenopus/Yeast | 適用 | IC,IF,IHC,IP,Western Blot | ||||
標識 | Unlabeled | 性状 | Purified | ||||
吸収処理 | クラス | IgG | |||||
クロナリティ | Polyclonal | フォーマット | |||||
掲載カタログ |
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製品記事 | ローディングコントロール抗体 使いっきり抗体 |
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関連記事 | GeneTex社における抗体の品質管理 |
Anti-β-Actin, Rabbit-Poly <Anti-ACTB>
文献数: 614
- 商品コード:GTX109639
- メーカー:GNT
- 包装:100μl
- 価格:¥85,000
- 在庫:1個
- 納期:10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 | レビューあり。KO/KDバリデーション済み抗体。抗原:aa 359~368 別名:actin beta,BRWS1,PS1TP5BP1 Genbank No: 60 |
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別包装品 | 別包装品あり | ||||||
法規制等 | |||||||
保存条件 | -20℃ | 法規備考 | |||||
抗原種 | Human | 免疫動物 | Rabbit | ||||
交差性 | Caenorhabditis elegans/Candida albicans/Cat/Chicken/Dog/Drosophila/E. coli/Hamster/Horse/Human/Monkey/Mosquito/Mouse/Pig/Plant/Rat/Rice/Sheep/Treeshrew/Worm/Xenopus/Yeast | 適用 | IC,IF,IHC,IP,Western Blot | ||||
標識 | Unlabeled | 性状 | Purified | ||||
吸収処理 | クラス | IgG | |||||
クロナリティ | Polyclonal | フォーマット | |||||
掲載カタログ |
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製品記事 | GeneTex社 オルガネラマーカー抗体 ローディングコントロール抗体 |
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関連記事 | GeneTex社における抗体の品質管理 |
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DATA IMAGES
![]() | Sample (30 μg of whole cell lysate) A: 293T B: NTH-3T3 C: mouse brain D: PC-12 E: Rat brain F: drosophila 10% SDS PAGE GTX109639 diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
![]() | Sample (whole cell lysate) A: Candida albicans (sc5314) 20ug B: Candida albicans (sc5314) 40ug 10% SDS PAGE GTX109639 diluted at 1:4000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
![]() | Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using beta Actin(GTX109639) antibody (Green) at 1:200 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000. |
![]() | Sample (30 μg of whole cell lysate) A: 293T B: A431 C: Jurkat D: Raji 10% SDS PAGE GTX109639 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
![]() | beta Actin antibody detects ACTB protein by western blot analysis. A. 50 μg mouse brain lysate/extract B. 50 μg mouse kidney lysate/extract C. 50 μg mouse lung lysate/extract D. 50 μg mouse testis lysate/extract 10% SDS-PAGE beta Actin antibody (GTX109639) dilution: 1:10000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
![]() | Sample (30 μg of whole cell lysate) A: Jurkat B: Raji C: 293T D: A431 E: HeLa F: HepG2 G: H1299 H: HCT116 I: MCF-7 J: NT2D1 K: PC-3 L: U87-MG 10% SDS PAGE GTX109639 diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
![]() | beta Actin antibody detects beta Actin protein at cytoplasm in rat testis by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. beta Actin antibody (GTX109639) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min |
![]() | ![]() Non-transfected (–) and transfected (+) 293T whole cell extracts (10 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX109639) diluted at 1:15000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
![]() | beta Actin antibody detects beta Actin protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. beta Actin antibody (GTX109639) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min |
![]() | Sample (whole cell lysate) A: 293T 20ug B: 293T 10ug C: 293T 5ug D: 293T 1ug 10% SDS PAGE GTX109639 diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
![]() | Immunofluorescence analysis of methanol-fixed HeLa, using Beta-actin (GTX109639) antibody at 1:500 dilution. |
![]() | Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using Beta actin(GTX109639) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min |
![]() | beta Actin antibody detects ACTB protein by western blot analysis. A. 30 μg PC-12 whole cell lysate/extract B. 30 μg Rat-2 whole cell lysate/extract 10% SDS-PAGE beta Actin antibody (GTX109639) dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
![]() | Sample (10 μg of whole cell lysate) A: Yeast lysate 7.5% SDS PAGE GTX109639 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
![]() | The WB analysis of beta Actin antibody was published by Dai YC and colleagues in the journal PLoS One in 2017 . PMID: 29016672 |
![]() | The WB analysis of beta Actin antibody was published by Cheng CW and colleagues in the journal Cell Death Dis in 2013 . PMID: 23788038 |
![]() | The WB analysis of beta Actin antibody was published by Cheng CW and colleagues in the journal Cell Death Dis in 2013 . PMID: 23788038 |
![]() | The WB analysis of beta Actin antibody was published by Cheng CW and colleagues in the journal Cell Death Dis in 2013 . PMID: 23788038 |
![]() | The WB analysis of beta Actin antibody was published by Cheng CW and colleagues in the journal Cell Death Dis in 2013 . PMID: 23788038 |
![]() | The WB analysis of beta Actin antibody was published by Nagai-Okatani C and colleagues in the journal PLoS One in 2016.PMID: 27281159 |
![]() | The WB analysis of beta Actin antibody was published by Syu JS and colleagues in the journal Sci Rep in 2017.PMID: 28325912 |
![]() | The WB analysis of beta Actin antibody was published by Chen NC and colleagues in the journal Sci Rep in 2016.PMID: 26838546 |
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製品情報
Host | Rabbit |
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Clonality | Polyclonal |
Isotype | IgG |
Application | WB, ICC/IF, IHC-P, IP |
Reactivity | Human, Mouse, Rat, Yeast, Sheep, Drosophila, Cat, Dog, Hamster, Chicken, Pig, Caenorhabditis elegans, Candida albicans, E. coli, Horse, Rice, Xenopus, Treeshrew, Plant, Worm |
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APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.Application | Dilution |
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WB | 1:500-1:20000 |
ICC/IF | 1:100-1:1000 |
IHC-P | 1:100-1:1000 |
IP | Assay dependent |
Calculated MW | 42 kDa. ( Note ) |
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Positive Control | 293T , A341 , Jurkat , Raji , HeLa , HepG2 , H1299 , HCT116 , MCF-7 , NT2D1 , PC-3 , U87-MG , NIH-3T3 , mouse brain , PC-12 , Rat2 , Rat brain , drosophila , Yeast lysate , Candida albicans (sc5314) whole cell lysate , *Rice leaf , *mouse aorta , *H9C2 , *OKT3 , *mouse ileum tissue , *mouse heart tissue , *mouse skeletal muscle tissue , *mouse brain tissue , *human periodontal ligament |
Specificity/Sensitivity | IP/MS validation was supported by references (PMID:30377395) |
Predict Reactivity | Rabbit, Bovine, Guinea pig, Japanese Medaka, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity) |
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PROPERTIES
Form | Liquid |
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Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
Concentration | 0.09 mg/ml (Please refer to the vial label for the specific concentration.) |
Antigen Species | Human |
Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human beta Actin. The exact sequence is proprietary. |
Purification | Purified by antigen-affinity chromatography. |
Conjugation | Unconjugated |
Note | For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption. |
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TARGET
Synonyms | actin beta , BRWS1 , PS1TP5BP1 |
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Cellular Localization | Cytoplasm , cytoskeleton |
Background | This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq] |
Database | ・ Gene ID: 60 ACTB ・ UniProt: P60709 ACTB |
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REFERENCE
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