抗Histone H2A.X抗体 | Anti-Histone H2A.X antibody

掲載日情報:2020/04/02 現在Webページ番号:38676

GeneTex社の抗Histone H2A.X抗体 | Anti-Histone H2A.X antibodyです。

本製品は研究用です。研究用以外には使用できません。

GeneTex社 ホームページでユーザーレビューが閲覧できます


GeneTex社では製品を使用したユーザーからのレビューを各国から集め、GeneTex社ホームページで公開しています。各ユーザーの使用アプリケーション、試料の由来動物、希釈倍率、ブロッキング試薬といった実験条件, 実験結果画像など参考となる情報が掲載されています。
抗Histone H2A.X抗体(#GTX108272)のユーザーレビューはこちら。

Ratingrating star full rating star full rating star full rating star full rating star empty( Average 4 based on 1 users reviews)
Application
Western Blot(WB)rating star full rating star full rating star full rating star full rating star empty( Average 4 based on 1 users reviews)

特長

  • 高品質の抗体です。
  • 幅広い研究分野に関連する抗体を取り揃えています。
  • 使用されたアプリケーションや動物種などの情報が充実しています。
  • 多数の論文で使用実績がある信頼性の高い抗体です。

目次に戻る

価格

[在庫・価格 :2024年05月06日 18時55分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
詳細 商品名
  • 商品コード
  • メーカー
  • 包装
  • 価格
  • 在庫
  • 法規制等
納期 文献数
Anti-Histone H2A.X, Rabbit-Poly <Anti-H2AFX>
10日程度 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 8
説明文
レビューあり
別名:H2A histone family member X,H2A.X,H2A/X,H2AX
Genbank No: 3014
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Rabbit クラス IgG 標識 Unlabeled
交差性 Human/Mouse/Rat 適用 IC,IF,IHC,IP,Western Blot
クロナリティ Polyclonal フォーマット 性状 Purified 吸収処理
掲載カタログ

製品記事 抗ヒストン抗体/修飾ヒストン抗体
抗ヒストンH2A抗体
使いっきり抗体
関連記事 GeneTex社における抗体の品質管理
Anti-Histone H2A.X, Rabbit-Poly <Anti-H2AFX>
10日程度 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 8
説明文
レビューあり
別名:H2A histone family member X,H2A.X,H2A/X,H2AX
Genbank No: 3014
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Rabbit クラス IgG 標識 Unlabeled
交差性 Human/Mouse/Rat 適用 IC,IF,IHC,IP,Western Blot
クロナリティ Polyclonal フォーマット 性状 Purified 吸収処理
掲載カタログ

製品記事 抗ヒストン抗体/修飾ヒストン抗体
抗ヒストンH2A抗体
関連記事 GeneTex社における抗体の品質管理

[在庫・価格 :2024年05月06日 18時55分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。

Anti-Histone H2A.X, Rabbit-Poly <Anti-H2AFX>

文献数: 8

説明文 レビューあり
別名:H2A histone family member X,H2A.X,H2A/X,H2AX
Genbank No: 3014
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Rabbit
交差性 Human/Mouse/Rat 適用 IC,IF,IHC,IP,Western Blot
標識 Unlabeled 性状 Purified
吸収処理 クラス IgG
クロナリティ Polyclonal フォーマット
掲載カタログ

製品記事 抗ヒストン抗体/修飾ヒストン抗体
抗ヒストンH2A抗体
使いっきり抗体
関連記事 GeneTex社における抗体の品質管理

Anti-Histone H2A.X, Rabbit-Poly <Anti-H2AFX>

文献数: 8

説明文 レビューあり
別名:H2A histone family member X,H2A.X,H2A/X,H2AX
Genbank No: 3014
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Rabbit
交差性 Human/Mouse/Rat 適用 IC,IF,IHC,IP,Western Blot
標識 Unlabeled 性状 Purified
吸収処理 クラス IgG
クロナリティ Polyclonal フォーマット
掲載カタログ

製品記事 抗ヒストン抗体/修飾ヒストン抗体
抗ヒストンH2A抗体
関連記事 GeneTex社における抗体の品質管理



目次に戻る

DATA IMAGES

Histone H2A.X antibody detects Histone H2A.X protein at nucleus in human esophagus cancer by immunohistochemical analysis. Sample: Paraffin-embedded human esophagus cancer. Histone H2A.X antibody (GTX108272) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Histone H2A.X antibody detects Histone H2A.X protein at nucleus on mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. Histone H2A.X antibody (GTX108272) dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Histone H2A.X antibody detects Histone H2A.X protein at nucleus in human cervical cancer by immunohistochemical analysis. Sample: Paraffin-embedded human cervical cancer. Histone H2A.X antibody (GTX108272) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Histone H2A.X antibody detects Histone H2A.X protein at nucleus on mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. Histone H2A.X antibody (GTX108272) dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Histone H2A.X antibody detects Histone H2A.X protein at nucleus on rat hind brain by immunohistochemical analysis. Sample: Paraffin-embedded rat hind brain. Histone H2A.X antibody (GTX108272) dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using Histone H2A.X (GTX108272) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
Histone H2A.X antibody immunoprecipitates H2AFX protein in IP experiments.IP samples: Jurkat whole cell extractA. 40 μg Jurkat whole cell extractB. Control with 4 μg of preimmune Rabbit IgGC. Immunoprecipitation of H2AFX protein by 4 μg Histone H2A.X antibody (GTX108272)15 % SDS-PAGEThe immunoprecipitated H2AFX protein was detected by Histone H2A.X antibody (GTX108272) diluted at 1:2000.[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

Untreated (–) and treated (+) HCT116 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2A.X antibody (GTX108272) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2A.X antibody (GTX108272) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Histone H2A.X antibody detects Histone H2A.X protein by western blot analysis.A. 50 μg mouse brain extract12% SDS-PAGEHistone H2A.X antibody (GTX108272) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2A.X antibody (GTX108272) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Histone H2A.X antibody detects Histone H2A.X protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Histone H2A.X stained by Histone H2A.X antibody (GTX108272) diluted at 1:500.Red: phalloidin, a cytoskeleton marker, diluted at 1:100.Scale bar= 10 μm.
Histone H2A.X antibody detects Histone H2A.X protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human breast carcinoma.Histone H2A.X stained by Histone H2A.X antibody (GTX108272) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Histone H2A.X antibody detects Histone H2A.X protein by western blot analysis.A. 50 μg rat brain extract12% SDS-PAGEHistone H2A.X antibody (GTX108272) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2A.X antibody (GTX108272) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.


目次に戻る

製品情報

HostRabbit
ClonalityPolyclonal
IsotypeIgG
ApplicationWB, ICC/IF, IHC-P, IP
ReactivityHuman, Mouse, Rat


目次に戻る

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW 15 kDa. ( Note )
Positive Control 293T , HepG2 , HCT116 , HCT116 (30uM cisplatin for 24hr) , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , mouse brain , rat brain , PC-12 , Rat2 , *4T1
Predict Reactivity Rhesus Monkey(>80% identity)


目次に戻る

PROPERTIES

Form Liquid
Buffer 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration 0.42 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species Human
Immunogen Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Histone H2A.X. The exact sequence is proprietary.
Purification Purified by antigen-affinity chromatography.
Conjugation Unconjugated
Note For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.


目次に戻る

TARGET

Synonyms H2A histone family member X , H2A.X , H2A/X , H2AX
Cellular Localization Nucleus
Background Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq]
Database ・ Gene ID: 3014 H2AFX
・ UniProt: P16104 H2AFX


目次に戻る

REFERENCEE(抜粋)

Application ReferenceApplication/Reactivity
Lee CY et al. Nat Commun 2019; 10 (1) : 65 Promotion of homology-directed DNA repair by polyamines.Application : WB
Reactivity : Human
Yu CY et al. Eur J Pharmacol 2017; (Epub) Ovatodiolide isolated from Anisomeles indica induces cell cycle G2/M arrest and apoptosis via a ROS-dependent ATM/ATR signaling pathways.Application : WB
Reactivity : Human
Liu MK et al. Int J Mol Sci 2016; 17 (6) Topoisomerase II Inhibitors Can Enhance Baculovirus-Mediated Gene Expression in Mammalian Cells through the DNA Damage Response.Application : WB
Reactivity : Human
Peraldo-Neia C et al. BMC Cancer 2014; 14 : 918 Anti-cancer effect and gene modulation of ET-743 in human biliary tract carcinoma preclinical models.Application : WB
Reactivity : Human
Chinnam M et al. J Natl Cancer Inst 2014; 106 (11) : pii: dju306 The Thoc1 ribonucleoprotein and prostate cancer progression.Application : WB


目次に戻る

お問い合わせ先

(テクニカルサポート 試薬担当)

reagent@funakoshi.co.jp

製品情報は掲載時点のものですが、価格表内の価格については随時最新のものに更新されます。お問い合わせいただくタイミングにより製品情報・価格などは変更されている場合があります。
表示価格に、消費税等は含まれていません。一部価格が予告なく変更される場合がありますので、あらかじめご了承下さい。