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抗NRF2 [N2C2], Internal抗体 | Anti-NRF2 [N2C2], Internal antibody

掲載日情報：2020/04/02 現在Webページ番号：36479

ジーンテックス /
GeneTex International Corporation
[メーカー略称：GNT]

GeneTex社の抗NRF2 [N2C2], Internal抗体 | Anti-NRF2 [N2C2], Internal antibodyです。

※ 本製品は研究用です。研究用以外には使用できません。

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特長

  • 高品質の抗体です。
  • 幅広い研究分野に関連する抗体を取り揃えています。
  • 使用されたアプリケーションや動物種などの情報が充実しています。
  • 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

[在庫・価格 ：2025年06月19日 12時15分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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 納期 文献数
Anti-NFE2L2, Rabbit-Poly
  • 使用文献
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  • お問い合わせ
 10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 113
  • 使用文献
  • メーカーサイト
  • お問い合わせ
説明文
レビューあり。
別名：nuclear factor，erythroid 2 like 2，HEBP1，IMDDHH，NRF2
Genbank No： 4780
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Rabbit クラス IgG 標識 Unlabeled
交差性 Alligator/Bird/Human/Mouse/Plant/Rat/Zebrafish 適用 ChIP,IC,IF,IHC,IP,Western Blot
クロナリティ Polyclonal フォーマット 性状 Purified 吸収処理
掲載カタログ

製品記事 使いっきり抗体
関連記事 GeneTex社における抗体の品質管理
Anti-NFE2L2, Rabbit-Poly
  • 使用文献
  • メーカーサイト
  • お問い合わせ
 10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 113
  • 使用文献
  • メーカーサイト
  • お問い合わせ
説明文
レビューあり。
別名：nuclear factor，erythroid 2 like 2，HEBP1，IMDDHH，NRF2
Genbank No： 4780
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Rabbit クラス IgG 標識 Unlabeled
交差性 Alligator/Bird/Human/Mouse/Plant/Rat/Zebrafish 適用 ChIP,IC,IF,IHC,IP,Western Blot
クロナリティ Polyclonal フォーマット 性状 Purified 吸収処理
掲載カタログ

製品記事
関連記事 GeneTex社における抗体の品質管理

[在庫・価格 ：2025年06月19日 12時15分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。

Anti-NFE2L2, Rabbit-Poly

文献数： 113

説明文 レビューあり。
別名：nuclear factor，erythroid 2 like 2，HEBP1，IMDDHH，NRF2
Genbank No： 4780
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Rabbit
交差性 Alligator/Bird/Human/Mouse/Plant/Rat/Zebrafish 適用 ChIP,IC,IF,IHC,IP,Western Blot
標識 Unlabeled 性状 Purified
吸収処理 クラス IgG
クロナリティ Polyclonal フォーマット
掲載カタログ

製品記事 使いっきり抗体
関連記事 GeneTex社における抗体の品質管理
  • 使用文献
  • メーカーサイト
  • お問い合わせ

Anti-NFE2L2, Rabbit-Poly

文献数： 113

説明文 レビューあり。
別名：nuclear factor，erythroid 2 like 2，HEBP1，IMDDHH，NRF2
Genbank No： 4780
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Rabbit
交差性 Alligator/Bird/Human/Mouse/Plant/Rat/Zebrafish 適用 ChIP,IC,IF,IHC,IP,Western Blot
標識 Unlabeled 性状 Purified
吸収処理 クラス IgG
クロナリティ Polyclonal フォーマット
掲載カタログ

製品記事
関連記事 GeneTex社における抗体の品質管理
  • 使用文献
  • メーカーサイト
  • お問い合わせ



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DATA IMAGES


Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with NRF2 antibody [N2C2], Internal (GTX103322) diluted at 1:500 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.*The competitor is not affiliated with GeneTex and does not endorse this product.
Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [N2C2], Internal (GTX103322) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Immunoprecipitation of NRF2 protein from HepG2 whole cell extracts using 5 μg of NRF2 antibody [N2C2], Internal (GTX103322).Western blot analysis was performed using NRF2 antibody [N2C2], Internal (GTX103322) diluted at 1:500.EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [N2C2], Internal (GTX103322) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [N2C2], Internal (GTX103322) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Untreated (–) and treated (+) MDA-MB-231 nuclear extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [N2C2], Internal (GTX103322) diluted at 1:1000.

Untreated (–) and treated (+) Neuro2A whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [N2C2], Internal (GTX103322) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Untreated (–) and treated (+) Rat-2 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [N2C2], Internal (GTX103322) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

NRF2 antibody detects NRF2 protein by western blot analysis. Non-transfected (-) and NRF2-transfected (+, including 3xFlag-tag) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody (GTX103322) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
ChIP was performed with HepG2 chromatin extract and 5 μg of either normal rabbit IgG or anti-NRF2 antibody. The precipitated DNA was detected by PCR with primer set targeting to GCLC gene locus.
NRF2 antibody [N2C2], Internal detects NRF2 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: NIH/3T3 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: NRF2 protein stained by NRF2 antibody [N2C2], Internal (GTX103322) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 μm.
NRF2 antibody [N2C2], Internal detects NRF2 protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human breast carcinoma.NRF2 stained by NRF2 antibody [N2C2], Internal (GTX103322) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Untreated (–) and treated (+) RAW264.7 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [N2C2], Internal (GTX103322) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
NRF2 antibody [N2C2], Internal detects NRF2 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: NRF2 stained by NRF2 antibody [N2C2], Internal (GTX103322) diluted at 1:500.Red: phalloidin, a cytoskeleton marker, diluted at 1:100.

Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [N2C2], Internal (GTX103322) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [N2C2], Internal (GTX103322) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.


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製品情報

HostRabbit
ClonalityPolyclonal
IsotypeIgG
ApplicationWB, ICC/IF, IHC-P, IP, ChIP assay, IHC
ReactivityHuman, Mouse, Rat, Zebrafish, Alligator, Plant, Bird


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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
ChIP assay Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW 68 kDa. ( Note )
Observed MW 110 kDa. The observed M.W. is based on the following publication. PMID: 22703241
Positive Control C8D30 , 3xFlag-human NFE2L2-transfected 293T , MDA-MB-231 nuclear extract , MDA-MB-231 nuclear extract (30uM tBHQ, 4hr) , HepG2 (10 μM MG132 treatment for 15 hr)
Predict Reactivity Bovine, Rhesus Monkey(>80% identity)


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PROPERTIES

Form Liquid
Buffer 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Storage Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration 0.36 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species Human
Immunogen Recombinant protein encompassing a sequence within the center region of human NRF2. The exact sequence is proprietary.
Purification Purified by antigen-affinity chromatography.
Conjugation Unconjugated
Note For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.


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TARGET

Synonyms nuclear factor, erythroid 2 like 2 , HEBP1 , IMDDHH , NRF2
Cellular Localization Cytoplasm , cytosol , Nucleus
Background NFE2 (MIM 601490), NFE2L1 (MIM 163260), and NFE2L2 comprise a family of human genes encoding basic leucine zipper (bZIP) transcription factors. They share highly conserved regions that are distinct from other bZIP families, such as JUN (MIM 165160) and FOS (MIM 164810), although remaining regions have diverged considerably from each other (Chan et al., 1995 [PubMed 7868116]).[supplied by OMIM]
Database ・ Gene ID: 4780 NFE2L2
・ UniProt: Q16236 NFE2L2


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REFERENCEE(抜粋)

Application ReferenceApplication/Reactivity
Ungerer G et al. Neuromolecular Med 2020; (Epub) Harpagophytum procumbens Extract Ameliorates Allodynia and Modulates Oxidative and Antioxidant Stress Pathways in a Rat Model of Spinal Cord Injury.Application : WB
Reactivity : Rat
Caihong Wang et al. Chemosphere 2020; 241 Triclosan-induced liver and brain injury in zebrafish (Danio rerio) via abnormal expression of miR-125 regulated by PKCα/Nrf2/p53 signaling pathways.Application : IHC-P
Reactivity : Zebrafish
Geng X et al. Mol Neurobiol 2019; (Epub) Effects of Docosahexaenoic Acid and Its Peroxidation Product on Amyloid-帣 Peptide-Stimulated Microglia.Application : WB
Reactivity : Mouse
Gandhi KA et al. Free Radic Biol Med 2019; 145 : 8-19 Oral administration of 3,3'-diselenodipropionic acid prevents thoracic radiation induced pneumonitis in mice by suppressing NF-kB/IL-17/G-CSF/neutrophil axis.Application : IHC-P
Reactivity : Mouse
Rashidi Z et al. Reprod Biol 2019; Quercetin protects human granulosa cells against oxidative stress via thioredoxin system.Application : WB
Reactivity : Human


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お問い合わせ先

（テクニカルサポート 試薬担当）

reagent@funakoshi.co.jp

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