Anti-SQSTM1, Rabbit-Poly | GeneTex International Corporation

GTX100685 WB Image
Untreated (?) and treated (+) HepG2 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SQSTM1 antibody [N3C1], Internal (GTX100685) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100685 ICC/IF Image
SQSTM1 antibody [N3C1], Internal detects SQSTM1 protein at autophagosome by immunofluorescent analysis.
Samples: HepG2 cells treated with 3uM thapsigargin 12 hrs (rigtht) and mock (left) were fixed in ice-cold MeOH for 10 min, permeabilize with cooled acetone for 1 min .
Green: SQSTM1 protein stained by SQSTM1 antibody [N3C1], Internal (GTX100685) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 um.

GTX100685 IP Image
Immunoprecipitation of SQSTM1 protein from HeLa whole cell extracts using 5 ug of SQSTM1 antibody [N3C1], Internal (GTX100685).
Western blot analysis was performed using SQSTM1 antibody [N3C1], Internal (GTX100685).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX100685 ICC/IF Image
SQSTM1 antibody [N3C1], Internal detects SQSTM1 protein at autophagosome by immunofluorescent analysis.
Samples: HeLa cells mock (left) and treated with 50uM Chloroquine for 24 hr (right) were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SQSTM1 protein stained by SQSTM1 antibody [N3C1], Internal (GTX100685) diluted at 1:1000.
Red: Phalloidin, a F-actin marker.

GTX100685 IHC-P Image
SQSTM1 antibody [N3C1], Internal detects SQSTM1 protein at cytoplasm on human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian carcinoma.
SQSTM1 antibody [N3C1], Internal (GTX100685) diluted at 1:500.

GTX100685 FACS Image
SQSTM1 antibody [N3C1], Internal (GTX100685) detects SQSTM1 protein by flow cytometry analysis.
Sample: HeLa cell fixed in 4% paraformaldehyde at 4oC for 5 min.
Brown: Unlabelled sample was also used as a control.
Blue: SQSTM1 antibody [N3C1], Internal] (GTX100685) dilution: 1:100.
Acquisition of >20,000 events were collected using Argon ion laser (488nm) and 525/30 bandpass filter.

GTX100685 WB Image
Non-transfected (?) and transfected (+) HepG2 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SQSTM1 antibody [N3C1], Internal (GTX100685) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100685 WB Image
Untreated (?) and treated (+) Huh-7 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SQSTM1 antibody [N3C1], Internal (GTX100685) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100685 WB Image
Sample (30 ug of whole cell lysate)
A: A549
10% SDS PAGE
GTX100685 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100685 WB Image
SQSTM1 antibody [N3C1], Internal detects SQSTM1 protein by western blot analysis.
A. 30 ug NIH-3T3 whole cell lysate/extract
B. 30 ug JC whole cell lysate/extract
C. 30 ug BCL-1 whole cell lysate/extract
12% SDS-PAGE
SQSTM1 antibody [N3C1], Internal (GTX100685) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100685 WB Image
SQSTM1 antibody [N3C1], Internal detects SQSTM1 protein by western blot analysis.
A. 30 ug PC-12 whole cell lysate/extract
B. 30 ug Rat2 whole cell lysate/extract
10% SDS-PAGE
SQSTM1 antibody [N3C1], Internal (GTX100685) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.