抗Oct4抗体 | Anti-Oct4 antibody

• 2024/11/15

  試薬 特別価格

  [GNT]一次抗体全製品25％OFFキャンペーン[～2025/02/28]

  期間：2024/11/15 ～2025/02/28

  GeneTex International Corporation（GNT）

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET
• REFERENCEE(抜粋)

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	Oct4 antibody immunoprecipitates Oct4 protein in IP experiments. IP Sample: cell lysate/extract of Oct4 gene transfected 293T cells A. Cell lysate/extract of transfected 293T cell B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of Oct4 by 2 μg of Oct4 antibody (GTX100622) 12% SDS-PAGE The immunoprecipitated Oct4 protein was detected by Oct4 antibody (GTX100622) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
	Immunofluorescence analysis of paraformaldehyde-fixed human embryonic stem cell, using Oct4(GTX100622) antibody at 1:200 dilution.
	Immunofluorescence analysis of paraformaldehyde-fixed Human ESC, using Oct4(GTX100622) antibody at 1:400 dilution.
	Flow cytometry on human embryonic stem cells, staining with Oct4 (GTX100622)antibody at 1:50 dilution(green) or rabbit IgG (black).
	Sample (20 μg of whole cell lysate) A: mouse ESC 12% SDS PAGE GTX100622 diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
	Immunofluorescence analysis of paraformaldehyde-fixed human embryonic stem cell, using Oct4(GTX100622) antibody at 1:100 dilution.
	Human tissue extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Oct4 antibody (GTX100622) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.The observed M.W. is based on the publication: PMID: 23642364

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製品情報

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, FACS, IP
Reactivity	Human, Mouse

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:1000-1:20000
ICC/IF	1:100-1:1000
FACS	1:50-1:200
IP	1:500-1:1000

Not tested in other applications.

Calculated MW	39 kDa. ( Note )
Positive Control	293T , A431 , Jurkat , Raji , HeLa nucleus , Human ESC , mouse ESC
Predict Reactivity	Rabbit, Bovine, Cat, Pig, Chimpanzee, Rhesus Monkey(>80% identity)

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PROPERTIES

Form	Liquid
Buffer	1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	Recombinant protein encompassing a sequence within the center region of human Oct4. The exact sequence is proprietary.
Purification	Purified by antigen-affinity chromatography.
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	POU class 5 homeobox 1 , OCT3 , OCT4 , OTF-3 , OTF3 , OTF4 , Oct-3 , Oct-4
Cellular Localization	Nucleus
Background	This gene encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene, t(6;22)(p21;q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. [provided by RefSeq]
Database	・ Gene ID: 5460 POU5F1 ・ UniProt: Q01860 POU5F1

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REFERENCEE(抜粋)

Application Reference	Application/Reactivity
O'Day E et al. J Biol Chem 2015; 290 (29) : 17909-22 An RNA-binding Protein, Lin28, Recognizes and Remodels G-quartets in the MicroRNAs (miRNAs) and mRNAs It Regulates.
Chen PM et al. Neoplasia 2014; 16 (11) : 961-71 Up-Regulation of FOXM1 by E6 Oncoprotein through the MZF1/NKX2-1 Axis Is Required for Human Papillomavirus-Associated Tumorigenesis.	Application : WB Reactivity : Human

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