抗Human Endoglin/CD105 Allophycocyanin抗体(Anti-Human Endoglin/CD105 Allophycocyanin antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Allophycocyanin
Immunogen	Mouse myeloma cell line NS0-derived recombinant human Endoglin/CD105Glu26-Gly586Accession # Q5T9B9
Source	Monoclonal Mouse IgG1 Clone # 166707
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human Endoglin/CD105.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	10 µL/106 cells	See below

Flow Cytometry
	Detection of Endoglin/CD105 in U937 Human Cell Line by Flow Cytometry. U937 human histiocytic lymphoma cell line was stained with Mouse Anti-Human Endoglin/CD105 APC-conjugated Mono­clonal Antibody (Catalog # FAB10971A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Related Product & Information

Background

Endoglin/CD105

background_content

Background: Endoglin/CD105 Endoglin (CD105) is a 90 kDa type I transmembrane glycoprotein of the zona pellucida (ZP) family of proteins (1‑3). Endoglin and betaglycan/T beta RIII are type III receptors for TGF beta superfamily ligands, sharing 71% aa identity in the transmembrane (TM) and cytoplasmic domains. Endoglin is highly expressed on proliferating vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta, with lower amounts on hematopoietic, mesenchymal and neural crest stem cells, activated monocytes, and lymphoid and myeloid leukemic cells (2‑5). Human Endoglin cDNA encodes 658 amino acids (aa) including a 25 aa signal sequence, a 561 aa extracellular domain (ECD) with an orphan domain and a two-part ZP domain, a TM domain and a 47 aa cytoplasmic domain (1‑3). An isoform with a 14 aa cytoplasmic domain (S-endoglin) can oppose effects of long (L) Endoglin (6, 7). The human Endoglin ECD shares 65-72% aa identity with mouse, rat, bovine, porcine and canine Endoglin. Endoglin homodimers interact with TGF-beta 1 and TGF-beta 3 (but not TGF-beta 2), but only after binding T beta RII (8). Similarly, they interact with activin-A and BMP-7 via activin type IIA or B receptors, and with BMP-2 via BMPR-1A/ALK-3 or BMPR-1B/ALK-6 (9). BMP-9, however, is reported to bind Endoglin directly (10). Endoglin modifies ligand-induced signaling in multiple ways. For example, expression of Endoglin can inhibit TGF-beta 1 signals but enhance BMP7 signals in the same myoblast cell line (11). In endothelial cells, Endoglin inhibits T beta RI/ALK5, but enhances ALK1-mediated activation (12). Deletion of mouse Endoglin causes lethal vascular and cardiovascular defects, and human Endoglin haploinsufficiency can a cause the vascular disorder, hereditary hemorrhagic telangiectasia type I (13, 14). These abnormalities confirm the essential function of Endoglin in differentiation of smooth muscle, angiogenesis, and neovascularization (2‑4, 12‑14). In preeclampsia of pregnancy, high levels of proteolytically generated soluble Endoglin and VEGF R1 (sFLT1), along with low placental growth factor (PlGF), are pathogenic due to antiangiogenic activity (15).

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Citations

1. Mesenchymal stromal cells (MSC) from JAK2+ myeloproliferative neoplasms differ from normal MSC and contribute to the maintenance of neoplastic hematopoiesis
   Authors: TL Ramos, LI Sánchez-Ab, B Rosón-Burg, A Redondo, A Rico, S Preciado, R Ortega, C Rodríguez, S Muntión, Á Hernández-, J De Las Riv, M González, JR González P, C Del Cañizo, F Sánchez-Gu
   PLoS ONE, 2017;12(8):e0182470.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


2. Microvesicles from Mesenchymal Stromal Cells Are Involved in HPC-Microenvironment Crosstalk in Myelodysplastic Patients.
   Authors: Muntion S, Ramos T, Diez-Campelo M, Roson B, Sanchez-Abarca L, Misiewicz-Krzeminska I, Preciado S, Sarasquete M, De Las Rivas J, Gonzalez M, Sanchez-Guijo F, Del Canizo M
   PLoS ONE, 2016;11(2):e0146722.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


3. MSC surface markers (CD44, CD73, and CD90) can identify human MSC-derived extracellular vesicles by conventional flow cytometry.
   Authors: L Ramos T, Sanchez-Abarca L, Muntion S, Preciado S, Puig N, Lopez-Ruano G, Hernandez-Hernandez A, Redondo A, Ortega R, Rodriguez C, Sanchez-Guijo F, Del Canizo C
   Cell Commun Signal, 2016;14(1):2.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


4. Effects of long-term hypoxia and pro-survival cocktail in bone marrow-derived stromal cell survival.
   Authors: Kim S, Chaudhry A, Lee I, Frank J
   Stem Cells Dev, 2014;23(5):530-40.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


5. Efficient differentiation of human pluripotent stem cells into mesenchymal stem cells by modulating intracellular signaling pathways in a feeder/serum-free system.
   Authors: Tran NT, Trinh QM, Lee GM
   Stem Cells Dev., 2012;21(7):1165-75.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow

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